Transcriptomic analysis of polysaccharide utilization loci reveals substrate preferences in ruminal generalists Segatella bryantii TF1-3 and Xylanibacter ruminicola KHP1.
Prevotella
Segatella
Xylanibacter
Polysaccharide utilization locus
Rumen
Transcriptomics
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
20 May 2024
20 May 2024
Historique:
received:
05
12
2023
accepted:
15
05
2024
medline:
21
5
2024
pubmed:
21
5
2024
entrez:
20
5
2024
Statut:
epublish
Résumé
Bacteria of the genera Xylanibacter and Segatella are among the most dominant groups in the rumen microbiota. They are characterized by the ability to utilize different hemicelluloses and pectin of plant cell-wall as well as plant energy storage polysaccharides. The degradation is possible with the use of cell envelope bound multiprotein apparatuses coded in polysaccharide utilization loci (PULs), which have been shown to be substrate specific. The knowledge of PUL presence in rumen Xylanibacter and Segatella based on bioinformatic analyses is already established and transcriptomic and genetic approaches confirmed predicted PULs for a limited number of substrates. In this study, we transcriptomically identified additional different PULs in Xylanibacter ruminicola KHP1 and Segatella bryantii TF1-3. We also identified substrate preferences and found that specific growth rate and extent of growth impacted the choice of substrates preferentially used for degradation. These preferred substrates were used by both strains simultaneously as judged by their PUL upregulation. Lastly, β-glucan and xyloglucan were used by these strains in the absence of bioinformatically and transcriptomically identifiable PUL systems.
Identifiants
pubmed: 38769483
doi: 10.1186/s12864-024-10421-z
pii: 10.1186/s12864-024-10421-z
doi:
Substances chimiques
xyloglucan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
495Subventions
Organisme : Javna Agencija za Raziskovalno Dejavnost RS
ID : P4-0097
Organisme : Javna Agencija za Raziskovalno Dejavnost RS
ID : P4-0097
Informations de copyright
© 2024. The Author(s).
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