Meta-analysis of shotgun sequencing of gut microbiota in Parkinson's disease.
Journal
NPJ Parkinson's disease
ISSN: 2373-8057
Titre abrégé: NPJ Parkinsons Dis
Pays: United States
ID NLM: 101675390
Informations de publication
Date de publication:
21 May 2024
21 May 2024
Historique:
received:
18
02
2024
accepted:
10
05
2024
medline:
22
5
2024
pubmed:
22
5
2024
entrez:
21
5
2024
Statut:
epublish
Résumé
We aimed to identify gut microbial features in Parkinson's disease (PD) across countries by meta-analyzing our fecal shotgun sequencing dataset of 94 PD patients and 73 controls in Japan with five previously reported datasets from USA, Germany, China1, China2, and Taiwan. GC-MS and LC-MS/MS assays were established to quantify fecal short-chain fatty acids (SCFAs) and fecal polyamines, respectively. α-Diversity was increased in PD across six datasets. Taxonomic analysis showed that species Akkermansia muciniphila was increased in PD, while species Roseburia intestinalis and Faecalibacterium prausnitzii were decreased in PD. Pathway analysis showed that genes in the biosyntheses of riboflavin and biotin were markedly decreased in PD after adjusting for confounding factors. Five out of six categories in carbohydrate-active enzymes (CAZymes) were decreased in PD. Metabolomic analysis of our fecal samples revealed that fecal SCFAs and polyamines were significantly decreased in PD. Genes in the riboflavin and biotin biosyntheses were positively correlated with the fecal concentrations of SCFAs and polyamines. Bacteria that accounted for the decreased riboflavin biosynthesis in Japan, the USA, and Germany were different from those in China1, China2, and Taiwan. Similarly, different bacteria accounted for decreased biotin biosynthesis in the two country groups. We postulate that decreased SCFAs and polyamines reduce the intestinal mucus layer, which subsequently facilitates the formation of abnormal α-synuclein fibrils in the intestinal neural plexus in PD, and also cause neuroinflammation in PD.
Identifiants
pubmed: 38773112
doi: 10.1038/s41531-024-00724-z
pii: 10.1038/s41531-024-00724-z
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP23H02794
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22K17343
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP23K06412
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22K15394
Organisme : Ministry of Health, Labour and Welfare (Ministry of Health, Labour and Welfare, Japan)
ID : 23FC1014
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP22ek0109488
Organisme : National Center of Neurology and Psychiatry (NCNP)
ID : 5-6
Informations de copyright
© 2024. The Author(s).
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