Combining analytical techniques to assess the translocation of diesel particles across an alveolar tissue barrier in vitro.
A549 cells
Diesel particles (DEPs)
Lock-in thermography (LIT)
Translocation
Transmission electron microscopy (TEM)
Ultraviolet – visible (UV-VIS)
Journal
Particle and fibre toxicology
ISSN: 1743-8977
Titre abrégé: Part Fibre Toxicol
Pays: England
ID NLM: 101236354
Informations de publication
Date de publication:
22 May 2024
22 May 2024
Historique:
received:
05
01
2024
accepted:
25
04
2024
medline:
23
5
2024
pubmed:
23
5
2024
entrez:
22
5
2024
Statut:
epublish
Résumé
During inhalation, airborne particles such as particulate matter ≤ 2.5 μm (PM We could detect the translocated fraction of DEPs across the PET membranes with 3 μm pore sizes and without cells by TEM analysis, and determine the percentage of translocation at approximatively 37% by UV-VIS (LOD: 1.92 µg.mL We propose a combination of analytical techniques to assess the translocation of DEPs across lung tissues. Our results reveal a low percentage of translocation of DEPs across alveolar epithelial tissue in vitro and they correspond to in vivo findings. The combination approach can be applied to any traffic-generated particles, thus enabling us to understand their involvement in public health.
Sections du résumé
BACKGROUND
BACKGROUND
During inhalation, airborne particles such as particulate matter ≤ 2.5 μm (PM
RESULTS
RESULTS
We could detect the translocated fraction of DEPs across the PET membranes with 3 μm pore sizes and without cells by TEM analysis, and determine the percentage of translocation at approximatively 37% by UV-VIS (LOD: 1.92 µg.mL
CONCLUSION
CONCLUSIONS
We propose a combination of analytical techniques to assess the translocation of DEPs across lung tissues. Our results reveal a low percentage of translocation of DEPs across alveolar epithelial tissue in vitro and they correspond to in vivo findings. The combination approach can be applied to any traffic-generated particles, thus enabling us to understand their involvement in public health.
Identifiants
pubmed: 38778339
doi: 10.1186/s12989-024-00585-7
pii: 10.1186/s12989-024-00585-7
doi:
Substances chimiques
Vehicle Emissions
0
Particulate Matter
0
Polyethylene Terephthalates
0
Air Pollutants
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
26Subventions
Organisme : H2020 EU project ULtrafine particles from TRansportation- Health Assessment of Source (ULTRHAS))
ID : 955390
Organisme : H2020 EU project ULtrafine particles from TRansportation- Health Assessment of Source (ULTRHAS))
ID : 955390
Informations de copyright
© 2024. The Author(s).
Références
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