Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis.
Burkholderia cepacia complex
CHROMagar™
Chronic respiratory infections
Cystic fibrosis
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297
Informations de publication
Date de publication:
23 May 2024
23 May 2024
Historique:
received:
03
02
2024
accepted:
03
05
2024
medline:
23
5
2024
pubmed:
23
5
2024
entrez:
23
5
2024
Statut:
aheadofprint
Résumé
Burkholderia cepacia complex (BCC) are non-fermenting Gram-negative bacteria that can chronically colonize the lungs of people with cystic fibrosis (pwCF), causing a severe and progressive respiratory failure, post-transplant complications and epidemic outbreaks. Therefore, rapid and accurate identification of these bacteria is relevant for pwCF, in order to facilitate early eradication and prevent chronic colonization. However, BCCs are often quite difficult to detect on culture media as they have a slow growth rate and can be hidden by other fast-growing microorganisms, including Pseudomonas aeruginosa and filamentous fungi. We evaluated the sensitivity of CHROMagar™ B. cepacia agar using 11 isolates from a well-characterized BCC collection, using BCA agar (Oxoid, UK) as a gold standard. We also studied 180 clinical sputum samples to calculate positive (PPV) and negative (NPV) predictive values. Furthermore, we used three of the well-characterized BCC isolates to determine the limit of detection (LOD). Eleven isolates grew on CHROMagar™ B. cepacia at 37ºC after 48 h. The NPV and PPV of CHROMagar™ B. cepacia were 100% and 87.5%, respectively. The LOD of CHROMagar™ B. cepacia was around 1 × 10 CHROMagar™ B. cepacia agar proved to have a very good sensitivity and specificity for the detection of clinical BCCs. Moreover, the chromogenic nature of the medium allowed us to clearly differentiate BCC from other Gram-negative species, filamentous fungi and yeasts, thereby facilitating the identification of contaminants.
Identifiants
pubmed: 38780755
doi: 10.1007/s10096-024-04845-4
pii: 10.1007/s10096-024-04845-4
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Instituto de Salud Carlos III
ID : Project PI19/01043
Organisme : Instituto de Salud Carlos III
ID : JR18/00034
Organisme : Instituto de Salud Carlos III
ID : CB21/13/00084
Informations de copyright
© 2024. The Author(s).
Références
Cullen L, Mcclean S (2015) Bacterial adaptation during chronic respiratory infections. Pathogens 4:66–89. https://doi.org/10.3390/pathogens4010066
doi: 10.3390/pathogens4010066
pubmed: 25738646
pmcid: 4384073
LiPuma JJ (2010) The changing microbial epidemiology in cystic fibrosis. Clin Microbiol Rev 23:299–323. https://doi.org/10.1128/CMR.00068-09
doi: 10.1128/CMR.00068-09
pubmed: 20375354
pmcid: 2863368
Rojas-Rojas FU, López-Sánchez D, Meza-Radilla G, Méndez-Canarios A, Ibarra JA, Estrada-de los Santos P (2019) The controversial Burkholderia cepacia complex, a group of plant growth promoting species and plant, animals and human pathogens. Rev Argent Microbiol 51:84–92. https://doi.org/10.1016/j.ram.2018.01.002
Medina-Pascual MJ, Valdezate S, Villalón P, Garrido N, Rubio V, Saéz-Nieto JA (2012) Identification, molecular characterization and antimicrobial susceptibility of genomovars of the Burkholderia cepacia complex in Spain. Eur J Clin Microbiol Infect Dis 31:3385–3396. https://doi.org/10.1007/s10096-012-1707-6
doi: 10.1007/s10096-012-1707-6
pubmed: 22855365
Mahenthiralingam E, Bischof J, Byrne SK, Radomski C, Davies JE, Av-Gay Y et al (2000) DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia Genomovars I and III. J Clin Microbiol 38:3165–3173. https://doi.org/10.1128/JCM.38.9.3165-3173.2000
doi: 10.1128/JCM.38.9.3165-3173.2000
pubmed: 10970351
pmcid: 87345
Vanlaere E, LiPuma JJ, Baldwin A, Henry D, de Brandt E, Mahenthiralingam E et al (2008) Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int J Syst Evol Microbiol 58:1580–1590. https://doi.org/10.1099/ijs.0.65634-0
doi: 10.1099/ijs.0.65634-0
pubmed: 18599699
Gonçalves PJR de O, Hume CCD, Ferreira AJ, Tsui S, Brocchi M, Wren BW, et al (2019) Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3. Sci Rep 9:5486. https://doi.org/10.1038/s41598-019-41778-x
Medina-Pascual MJ, Valdezate S, Carrasco G, Villalón P, Garrido N, Saéz-Nieto JA (2015) Increase in isolation of Burkholderia contaminans from Spanish patients with cystic fibrosis. Clin Microbiol Infect 21:150–156. https://doi.org/10.1016/j.cmi.2014.07.014
doi: 10.1016/j.cmi.2014.07.014
pubmed: 25658563
de Dios CJ, del Campo R, Royuela A, Solé A, Máiz L, Olveira C et al (2016) Bronchopulmonary infection–colonization patterns in Spanish cystic fibrosis patients: Results from a national multicenter study. J Cyst Fibros 15:357–365. https://doi.org/10.1016/j.jcf.2015.09.004
doi: 10.1016/j.jcf.2015.09.004
Rhodes KA, Schweizer HP (2016) Antibiotic resistance in Burkholderia Species. Drugs Resist Updat 28:82–90. https://doi.org/10.1016/j.drup.2016.07.003
doi: 10.1016/j.drup.2016.07.003
Mahenthiralingam E, Urban TA, Goldberg JB (2005) The multifarious, multireplicon Burkholderia cepacia complex. Nat Rev Microbiol 3:144–156. https://doi.org/10.1038/nrmicro1085
doi: 10.1038/nrmicro1085
pubmed: 15643431
Qvist T, Taylor-Robinson D, Waldmann E, Olesen HV, Hansen CR, Mathiesen IH et al (2016) Comparing the harmful effects of nontuberculous mycobacteria and Gram negative bacteria on lung function in patients with cystic fibrosis. J Cyst Fibros 15:380–385. https://doi.org/10.1016/j.jcf.2015.09.007
doi: 10.1016/j.jcf.2015.09.007
pubmed: 26482717
pmcid: 4893021
Gilligan PH (2014) Infections in patients with cystic fibrosis. Clin Lab Med 34:197–217. https://doi.org/10.1016/j.cll.2014.02.001
doi: 10.1016/j.cll.2014.02.001
pubmed: 24856524
pmcid: 7115738
Dingle TC, Butler-Wu SM (2013) MALDI-TOF mass spectrometry for microorganism identification. Clin Lab Med 33:589–609. https://doi.org/10.1016/j.cll.2013.03.001
doi: 10.1016/j.cll.2013.03.001
pubmed: 23931840
Vrioni G, Daniil I, Voulgari E, Ranellou K, Koumaki V, Ghirardi S et al (2012) Comparative evaluation of a prototype chromogenic medium (ChromID CARBA) for detecting carbapenemase-producing Enterobacteriaceae in surveillance rectal swabs. J Clin Microbiol 50:1841–1846. https://doi.org/10.1128/JCM.06848-11
doi: 10.1128/JCM.06848-11
pubmed: 22461675
pmcid: 3372115
Marrs ECL, Perry A, Perry JD (2021) Evaluation of three culture media for isolation of Burkholderia cepacia complex from respiratory samples of patients with cystic fibrosis. Microorganisms 9(12):2604. https://doi.org/10.3390/microorganisms9122604
doi: 10.3390/microorganisms9122604
pubmed: 34946206
pmcid: 8705780
Pérez-Viso B, Aracil-Gisbert S, Coque TM, Del Campo R, Ruiz-Garbajosa P, Cantón R (2021) Evaluation of CHROMagar™-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens. Eur J Clin Microbiol Infect Dis 40(12):2593–2596. https://doi.org/10.1007/s10096-021-04328-w
doi: 10.1007/s10096-021-04328-w
pubmed: 34363530
pmcid: 8590650
García-Fernández S, Hernández-García M, Valverde A, Ruiz-Garbajosa P, Morosini MI, Cantón R (2017) CHROMagar mSuperCARBA performance in carbapenem-resistant Enterobacteriaceae isolates characterized at molecular level and routine surveillance rectal swab specimens. Diagn Microbiol Infect Dis 87:207–209. https://doi.org/10.1016/j.diagmicrobio.2016.11.014
doi: 10.1016/j.diagmicrobio.2016.11.014
pubmed: 27916545
Whitby PW, Dick HLN, Campbell Iii PW, Tullis DE, Matlow A, Stull TL (1998) Comparison of culture and PCR for detection of Burkholderia cepacia in sputum samples of patients with cystic fibrosis. J Clin Microbiol 36:1642–1645. https://doi.org/10.1128/jcm.36.6.1642-1645.1998
doi: 10.1128/jcm.36.6.1642-1645.1998
pubmed: 9620393
pmcid: 104893
Drevinek P, Vosahlikova S, Dedeckova K, Cinek O, Mahenthiralingam E (2010) Direct culture-independent strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis. J Clin Microbiol 48:1888–1891. https://doi.org/10.1128/JCM.02359-09
doi: 10.1128/JCM.02359-09
pubmed: 20181898
pmcid: 2863923
Barrio Gómez De Agüero MI, García Hernández G, Gartner S, de La Cruz ÓA, Barroso NC, Montaner AE, et al (2009) Protocol for the diagnosis and follow up of patients with cystic fibrosis. An Pediatr 71:250–264. https://doi.org/10.1016/j.anpedi.2009.06.020