Highly variable biological effects of statins on cancer, non-cancer, and stem cells in vitro.
3-Hydroxy-3-methylglutaryl coenzyme A inhibitors
Cell spheroids
Microarray analysis
Pancreatic cancer cells
Statins
Stem cells
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 05 2024
23 05 2024
Historique:
received:
21
02
2024
accepted:
20
05
2024
medline:
24
5
2024
pubmed:
24
5
2024
entrez:
23
5
2024
Statut:
epublish
Résumé
Statins, the drugs used for the treatment of hypercholesterolemia, have come into the spotlight not only as chemoadjuvants, but also as potential stem cell modulators in the context of regenerative therapy. In our study, we compared the in vitro effects of all clinically used statins on the viability of human pancreatic cancer (MiaPaCa-2) cells, non-cancerous human embryonic kidney (HEK 293) cells and adipose-derived mesenchymal stem cells (ADMSC). Additionally, the effect of statins on viability of MiaPaCa-2 and ADMSC cells spheroids was tested. Furthermore, we performed a microarray analysis on ADMSCs treated with individual statins (12 μM) and compared the importance of the effects of statins on gene expression between stem cells and pancreatic cancer cells. Concentrations of statins that significantly affected cancer cells viability (< 40 μM) did not affect stem cells viability after 24 h. Moreover, statins that didn´t affect viability of cancer cells grown in a monolayer, induce the disintegration of cancer cell spheroids. The effect of statins on gene expression was significantly less pronounced in stem cells compared to pancreatic cancer cells. In conclusion, the low efficacy of statins on non-tumor and stem cells at concentrations sufficient for cancer cells growth inhibition, support their applicability in chemoadjuvant tumor therapy.
Identifiants
pubmed: 38782983
doi: 10.1038/s41598-024-62615-w
pii: 10.1038/s41598-024-62615-w
doi:
Substances chimiques
Hydroxymethylglutaryl-CoA Reductase Inhibitors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11830Subventions
Organisme : Slovak Research and Development Agency
ID : APVV-15-0217
Organisme : Ministry of Education, Research, Development and Youth of the Slovak Republic
ID : VEGA-1/0405/22
Organisme : Operational Programme Research, Development, and Education
ID : CZ.02.1.01/0.0/0.0/16_019/0000785
Organisme : Czech Ministry of Health
ID : MH CZ-DRO-VFN64165
Informations de copyright
© 2024. The Author(s).
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