The existence of cells exhibiting characteristics of both Type II and Type III cells in rat taste buds. An immunohistochemical and electron-microscopic study.

Cell type Electron microscopy Immunohistochemistry Rat Taste bud

Journal

Odontology
ISSN: 1618-1255
Titre abrégé: Odontology
Pays: Japan
ID NLM: 101134822

Informations de publication

Date de publication:
26 May 2024
Historique:
received: 09 02 2024
accepted: 04 05 2024
medline: 26 5 2024
pubmed: 26 5 2024
entrez: 26 5 2024
Statut: aheadofprint

Résumé

Taste bud cells are classified into four types by their ultrastructural features. Immunohistochemical detection of taste-signaling molecules is used to distinguish cell types of taste bud cells; however, the characteristics of taste cell types such as the immunoreactivity for taste-signaling molecules have long remained unclear. We investigated the detailed characteristics of taste cells in rat vallate taste buds by electron microscopy and immunohistochemistry for gustducin, neural cell adhesion molecule (NCAM) and vesicle-associated membrane protein 2 (VAMP2), which are known as markers of Type II cells, Type III cells and both cell types, respectively. Triple immunostaining for these molecules discriminated seven kinds of cell, including the totally immunopositive cell. Electron microscopy revealed Type III cells with a typical synaptic structure and subsurface cisterna as a specialized contact between a nerve and a Type II cell. The present study clarified the existence of cells with features of both Type II and Type III cells as a subtype of taste bud cells in the rat taste bud.

Identifiants

DOI: 10.1007/s10266-024-00948-8 PMID: 38796802
pubmed: 38796802
doi: 10.1007/s10266-024-00948-8
pii: 10.1007/s10266-024-00948-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : JSPS KAKENHI
ID : 20K10020

Informations de copyright

© 2024. The Author(s), under exclusive licence to The Society of The Nippon Dental University.

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Auteurs

Shuhei Inoko (S)

Histology, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-Cho, Chuo-Ku, Niigata, 951-8580, Japan.

Hiroki Katagiri (H)

Department of Histology, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-Cho, Chuo-Ku, Niigata, 951-8580, Japan.
Department of Microbiology, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan.
Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan.

Maiko Tsujimura (M)

Histology, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-Cho, Chuo-Ku, Niigata, 951-8580, Japan. h-maiko@ngt.ndu.ac.jp.
Department of Histology, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-Cho, Chuo-Ku, Niigata, 951-8580, Japan. h-maiko@ngt.ndu.ac.jp.
Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan. h-maiko@ngt.ndu.ac.jp.
ORCID: 0000-0001-8985-3015

Sumio Yoshie (S)

Department of Histology, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-Cho, Chuo-Ku, Niigata, 951-8580, Japan. yoshie@ngt.ndu.ac.jp.

Classifications MeSH