Development of a novel Colorimetric Assay for the rapid diagnosis of Coronavirus disease 2019 from nasopharyngeal samples.
Colorimetry
/ methods
Humans
COVID-19
/ diagnosis
Metal Nanoparticles
/ chemistry
Gold
/ chemistry
Nasopharynx
/ virology
SARS-CoV-2
/ isolation & purification
RNA, Viral
/ analysis
Sensitivity and Specificity
Limit of Detection
Oligonucleotide Probes
/ genetics
COVID-19 Nucleic Acid Testing
/ methods
Real-Time Polymerase Chain Reaction
/ methods
COVID-19 Testing
/ methods
AuNP-probe conjugate
Coronavirus disease 2019
Diagnosis
Real-Time PCR
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 May 2024
27 May 2024
Historique:
received:
14
11
2023
accepted:
04
02
2024
medline:
28
5
2024
pubmed:
28
5
2024
entrez:
27
5
2024
Statut:
epublish
Résumé
Emergence of Coronavirus disease 2019 (COVID-19) pandemic has posed a huge threat to public health. Rapid and reliable test to diagnose infected subjects is crucial for disease spread control. We developed a colorimetric test for COVID-19 detection using a Colorimetric Assay based on thiol-linked RNA modified gold nanoparticles (AuNPs) and oligonucleotide probes. This method was conducted on RNA from 200 pharyngeal swab samples initially tested by Real-Time polymerase chain reaction (RT-PCR) as gold standard. A specific oligonucleotide probe designed based on ORF1ab of COVID-19 was functionalized with AuNPs-probe conjugate. The exposure of AuNP-probe to isolated RNA samples was tested using hybridization. In this comparative study, the colorimetric functionalized AuNPs assay exhibited a detection limit of 25 copies/µL. It was higher in comparison to the RT-PCR method, which could only detect 15 copies/µL. The results demonstrated 100% specificity and 96% sensitivity for the developed method. Herein, we developed an incredibly rapid, simple and cost-effective Colorimetric Assay lasting approximately 30 min which could process considerably higher number of COVID-19 samples compared to the RT-PCR. This AuNP-probe conjugate colorimetric method could be considered the optimum alternatives for conventional diagnostic tools especially in over-populated and/or low-income countries.
Identifiants
pubmed: 38802360
doi: 10.1038/s41598-024-53747-0
pii: 10.1038/s41598-024-53747-0
doi:
Substances chimiques
Gold
7440-57-5
RNA, Viral
0
Oligonucleotide Probes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12125Informations de copyright
© 2024. The Author(s).
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