Structure classification of the proteins from Salmonella enterica pangenome revealed novel potential pathogenicity islands.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
28 May 2024
Historique:
received: 20 02 2024
accepted: 30 04 2024
medline: 29 5 2024
pubmed: 29 5 2024
entrez: 28 5 2024
Statut: epublish

Résumé

Salmonella enterica is a pathogenic bacterium known for causing severe typhoid fever in humans, making it important to study due to its potential health risks and significant impact on public health. This study provides evolutionary classification of proteins from Salmonella enterica pangenome. We classified 17,238 domains from 13,147 proteins from 79,758 Salmonella enterica strains and studied in detail domains of 272 proteins from 14 characterized Salmonella pathogenicity islands (SPIs). Among SPIs-related proteins, 90 proteins function in the secretion machinery. 41% domains of SPI proteins have no previous sequence annotation. By comparing clinical and environmental isolates, we identified 3682 proteins that are overrepresented in clinical group that we consider as potentially pathogenic. Among domains of potentially pathogenic proteins only 50% domains were annotated by sequence methods previously. Moreover, 36% (1330 out of 3682) of potentially pathogenic proteins cannot be classified into Evolutionary Classification of Protein Domains database (ECOD). Among classified domains of potentially pathogenic proteins the most populated homology groups include helix-turn-helix (HTH), Immunoglobulin-related, and P-loop domains-related. Functional analysis revealed overrepresentation of these protein in biological processes related to viral entry into host cell, antibiotic biosynthesis, DNA metabolism and conformation change, and underrepresentation in translational processes. Analysis of the potentially pathogenic proteins indicates that they form 119 clusters or novel potential pathogenicity islands (NPPIs) within the Salmonella genome, suggesting their potential contribution to the bacterium's virulence. One of the NPPIs revealed significant overrepresentation of potentially pathogenic proteins. Overall, our analysis revealed that identified potentially pathogenic proteins are poorly studied.

Identifiants

pubmed: 38806511
doi: 10.1038/s41598-024-60991-x
pii: 10.1038/s41598-024-60991-x
doi:

Substances chimiques

Bacterial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

12260

Subventions

Organisme : NIGMS NIH HHS
ID : GM147367
Pays : United States
Organisme : NIGMS NIH HHS
ID : GM127390
Pays : United States
Organisme : Welch Foundation
ID : I-2095-20220331
Organisme : Welch Foundation
ID : I-1505
Organisme : National Science Foundation
ID : DBI 2224128

Informations de copyright

© 2024. The Author(s).

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Auteurs

Kirill E Medvedev (KE)

Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA. Kirill.Medvedev@UTSouthwestern.edu.

Jing Zhang (J)

Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

R Dustin Schaeffer (RD)

Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

Lisa N Kinch (LN)

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

Qian Cong (Q)

Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

Nick V Grishin (NV)

Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

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