Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea.

Photosystem II Protein Complex / metabolism Cryptophyta / metabolism Cryoelectron Microscopy Chlorophyll / metabolism Chlorophyll Binding Proteins / metabolism Protein Multimerization Chlorophyll A / metabolism Models, Molecular Light-Harvesting Protein Complexes / metabolism

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
28 May 2024
Historique:
received: 29 11 2023
accepted: 15 05 2024
medline: 29 5 2024
pubmed: 29 5 2024
entrez: 28 5 2024
Statut: epublish

Résumé

Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 Å using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery.

Identifiants

DOI: 10.1038/s41467-024-48878-x PMID: 38806516
pubmed: 38806516
doi: 10.1038/s41467-024-48878-x
pii: 10.1038/s41467-024-48878-x
doi:

Substances chimiques

Photosystem II Protein Complex 0
Chlorophyll 1406-65-1
Chlorophyll Binding Proteins 0
Chlorophyll A YF5Q9EJC8Y
Light-Harvesting Protein Complexes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4535

Informations de copyright

© 2024. The Author(s).

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Auteurs

Zhiyuan Mao (Z)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.

Xingyue Li (X)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.

Zhenhua Li (Z)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.

Liangliang Shen (L)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
Cryo-EM Centre, Southern University of Science and Technology, 518055, Guangdong, China.
China National Botanical Garden, 100093, Beijing, China.
ORCID: 0000-0003-0868-3283

Xiaoyi Li (X)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
China National Botanical Garden, 100093, Beijing, China.

Yanyan Yang (Y)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
China National Botanical Garden, 100093, Beijing, China.

Wenda Wang (W)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
China National Botanical Garden, 100093, Beijing, China.
Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, 257300, Dongying, China.
ORCID: 0000-0002-1842-8633

Tingyun Kuang (T)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
China National Botanical Garden, 100093, Beijing, China.
Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, 257300, Dongying, China.
ORCID: 0000-0002-1885-4210

Jian-Ren Shen (JR)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China. shen@cc.okayama-u.ac.jp.
China National Botanical Garden, 100093, Beijing, China. shen@cc.okayama-u.ac.jp.
Institute for Interdisciplinary Science, and Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan. shen@cc.okayama-u.ac.jp.
ORCID: 0000-0003-4471-8797

Guangye Han (G)

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China. hanguangye@ibcas.ac.cn.
China National Botanical Garden, 100093, Beijing, China. hanguangye@ibcas.ac.cn.
Academician Workstation of Agricultural High-tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, 257300, Dongying, China. hanguangye@ibcas.ac.cn.
ORCID: 0000-0003-3373-2008

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines chloroplastiques › Protéines de la membrane thylakoïdale › Complexe protéique du centre réactionnel de la photosynthèse › Complexe protéique du photosystème II › Structure and distinct supramolecular...
questionsmedicales.fr › Eucaryotes › Cryptophyta › Structure and distinct supramolecular...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Structure and distinct supramolecular...
questionsmedicales.fr › Composés polycycliques › Composés macrocycliques › Tétrapyrroles › Chlorophylle › Structure and distinct supramolecular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines chloroplastiques › Protéines de la membrane thylakoïdale › Complexe protéique du centre réactionnel de la photosynthèse › Complexes collecteurs de lumière › Protéines liant la chlorophylle › Structure and distinct supramolecular...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › Structure and distinct supramolecular...
questionsmedicales.fr › Composés polycycliques › Composés macrocycliques › Tétrapyrroles › Chlorophylle › Chlorophylle A › Structure and distinct supramolecular...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structure and distinct supramolecular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines chloroplastiques › Protéines de la membrane thylakoïdale › Complexe protéique du centre réactionnel de la photosynthèse › Complexes collecteurs de lumière › Structure and distinct supramolecular...