Neurons dispose of hyperactive kinesin into glial cells for clearance.
Autoinhibition
Conformation
Glia
Hyperactive
Kinesin
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
28 May 2024
28 May 2024
Historique:
received:
22
11
2023
accepted:
25
04
2024
revised:
06
04
2024
medline:
29
5
2024
pubmed:
29
5
2024
entrez:
28
5
2024
Statut:
aheadofprint
Résumé
Microtubule-based kinesin motor proteins are crucial for intracellular transport, but their hyperactivation can be detrimental for cellular functions. This study investigated the impact of a constitutively active ciliary kinesin mutant, OSM-3CA, on sensory cilia in C. elegans. Surprisingly, we found that OSM-3CA was absent from cilia but underwent disposal through membrane abscission at the tips of aberrant neurites. Neighboring glial cells engulf and eliminate the released OSM-3CA, a process that depends on the engulfment receptor CED-1. Through genetic suppressor screens, we identified intragenic mutations in the OSM-3CA motor domain and mutations inhibiting the ciliary kinase DYF-5, both of which restored normal cilia in OSM-3CA-expressing animals. We showed that conformational changes in OSM-3CA prevent its entry into cilia, and OSM-3CA disposal requires its hyperactivity. Finally, we provide evidence that neurons also dispose of hyperactive kinesin-1 resulting from a clinic variant associated with amyotrophic lateral sclerosis, suggesting a widespread mechanism for regulating hyperactive kinesins.
Identifiants
pubmed: 38806659
doi: 10.1038/s44318-024-00118-0
pii: 10.1038/s44318-024-00118-0
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 31991191
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 32200612
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 32071191
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 31971160
Organisme : MOST | National Key Research and Development Program of China (NKPs)
ID : 2019YFA0508401
Organisme : Strategic Priority Research Program of CAS
ID : XDB37020302
Informations de copyright
© 2024. The Author(s).
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