Binding of Akkermansia muciniphila to mucin is O-glycan specific.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 May 2024
29 May 2024
Historique:
received:
05
11
2023
accepted:
09
05
2024
medline:
30
5
2024
pubmed:
30
5
2024
entrez:
29
5
2024
Statut:
epublish
Résumé
The intestinal anaerobic bacterium Akkermansia muciniphila is specialized in the degradation of mucins, which are heavily O-glycosylated proteins that constitute the major components of the mucus lining the intestine. Despite that adhesion to mucins is considered critical for the persistence of A. muciniphila in the human intestinal tract, our knowledge of how this intestinal symbiont recognizes and binds to mucins is still limited. Here, we first show that the mucin-binding properties of A. muciniphila are independent of environmental oxygen concentrations and not abolished by pasteurization. We then dissected the mucin-binding properties of pasteurized A. muciniphila by use of a recently developed cell-based mucin array that enables display of the tandem repeats of human mucins with distinct O-glycan patterns and structures. We found that A. muciniphila recognizes the unsialylated LacNAc (Galβ1-4GlcNAcβ1-R) disaccharide selectively on core2 and core3 O-glycans. This disaccharide epitope is abundantly found on human colonic mucins capped by sialic acids, and we demonstrated that endogenous A. muciniphila neuraminidase activity can uncover the epitope and promote binding. In summary, our study provides insights into the mucin-binding properties important for colonization of a key mucin-foraging bacterium.
Identifiants
pubmed: 38811534
doi: 10.1038/s41467-024-48770-8
pii: 10.1038/s41467-024-48770-8
doi:
Substances chimiques
Mucins
0
Polysaccharides
0
Neuraminidase
EC 3.2.1.18
Disaccharides
0
Epitopes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4582Subventions
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek (Netherlands Organisation for Scientific Research)
ID : 737.016.003
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek (Netherlands Organisation for Scientific Research)
ID : 024.002.002
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek (Netherlands Organisation for Scientific Research)
ID : Spinoza Grant Willem M de Vos
Organisme : Federation of European Microbiological Societies (FEMS)
ID : FEMS-GO-2021-069
Organisme : Novo Nordisk Fonden (Novo Nordisk Foundation)
ID : 0071658
Organisme : Lundbeckfonden (Lundbeck Foundation)
ID : NA
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : GlycoSkin H2020-ERC; 772735
Informations de copyright
© 2024. The Author(s).
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