Transcriptional control of the Cryptosporidium life cycle.


Journal

Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462

Informations de publication

Date de publication:
29 May 2024
Historique:
received: 07 06 2023
accepted: 25 04 2024
medline: 30 5 2024
pubmed: 30 5 2024
entrez: 29 5 2024
Statut: aheadofprint

Résumé

The parasite Cryptosporidium is a leading agent of diarrhoeal disease in young children, and a cause and consequence of chronic malnutrition

Identifiants

pubmed: 38811723
doi: 10.1038/s41586-024-07466-1
pii: 10.1038/s41586-024-07466-1
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature Limited.

Références

Kotloff, K. L. et al. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 382, 209–222 (2013).
pubmed: 23680352 doi: 10.1016/S0140-6736(13)60844-2
Khalil, I. A. et al. Morbidity, mortality, and long-term consequences associated with diarrhoea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study. Lancet Glob. Health 6, e758–e768 (2018).
pubmed: 29903377 pmcid: 6005120 doi: 10.1016/S2214-109X(18)30283-3
Checkley, W. et al. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for Cryptosporidium. Lancet Infect. Dis. 15, 85–94 (2015).
pubmed: 25278220 doi: 10.1016/S1473-3099(14)70772-8
English, E. D., Guerin, A., Tandel, J. & Striepen, B. Live imaging of the Cryptosporidium parvum life cycle reveals direct development of male and female gametes from type I meronts. PLoS Biol. 20, e3001604 (2022).
pubmed: 35436284 pmcid: 9015140 doi: 10.1371/journal.pbio.3001604
Striepen, B. Parasitic infections: time to tackle cryptosporidiosis. Nature 503, 189–191 (2013).
pubmed: 24236315 doi: 10.1038/503189a
Current, W. L. & Reese, N. C. A comparison of endogenous development of three isolates of Cryptosporidium in suckling mice. J. Protozool. 33, 98–108 (1986).
pubmed: 3959014 doi: 10.1111/j.1550-7408.1986.tb05567.x
Gharpure, R. et al. Cryptosporidiosis Outbreaks—United States, 2009–2017. MMWR Morb. Mortal. Wkly Rep. 68, 568–572 (2019).
pubmed: 31246941 pmcid: 6597118 doi: 10.15585/mmwr.mm6825a3
Tandel, J. et al. Life cycle progression and sexual development of the apicomplexan parasite Cryptosporidium parvum. Nat. Microbiol. 4, 2226–2236 (2019).
pubmed: 31477896 pmcid: 6877471 doi: 10.1038/s41564-019-0539-x
Huang, W. et al. Multiple introductions and recombination events underlie the emergence of a hyper-transmissible Cryptosporidium hominis subtype in the USA. Cell Host Microbe 31, 112–123 (2023).
pubmed: 36521488 doi: 10.1016/j.chom.2022.11.013
Nader, J. L. et al. Evolutionary genomics of anthroponosis in Cryptosporidium. Nat. Microbiol. 4, 826–836 (2019).
pubmed: 30833731 doi: 10.1038/s41564-019-0377-x
Guerin, A. & Striepen, B. The biology of the intestinal intracellular parasite Cryptosporidium. Cell Host Microbe 28, 509–515 (2020).
pubmed: 33031769 doi: 10.1016/j.chom.2020.09.007
Kissinger, J. C., Hermetz, K. E., Woods, K. M. & Upton, S. J. Enrichment of Cryptosporidium parvum from in vitro culture as measured by total RNA and subsequent sequence analysis. Mol. Biochem. Parasitol. 220, 5–9 (2018).
pubmed: 29292212 doi: 10.1016/j.molbiopara.2017.12.004
La Manno, G. et al. RNA velocity of single cells. Nature 560, 494–498 (2018).
pubmed: 30089906 pmcid: 6130801 doi: 10.1038/s41586-018-0414-6
Guerin, A. et al. Cryptosporidium uses multiple distinct secretory organelles to interact with and modify its host cell. Cell Host Microbe 31, 650–664 (2023).
pubmed: 36958336 doi: 10.1016/j.chom.2023.03.001
Vetterling, J. M., Jervis, H. R., Merrill, T. G. & Sprinz, H. Cryptosporidium wrairi sp. n. from the guinea pig Cavia porcellus, with an emendation of the genus. J. Protozool. 18, 243–247 (1971).
pubmed: 4997038 doi: 10.1111/j.1550-7408.1971.tb03315.x
Jumani, R. S. et al. A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors. Nat. Commun. 10, 1862 (2019).
pubmed: 31015448 pmcid: 6478823 doi: 10.1038/s41467-019-09880-w
Tandel, J. et al. Genetic ablation of a female-specific Apetala 2 transcription factor blocks oocyst shedding in Cryptosporidium parvum. mBio 14, e0326122 (2023).
pubmed: 36786597 doi: 10.1128/mbio.03261-22
Li, Y., Baptista, R. P., Sateriale, A., Striepen, B. & Kissinger, J. C. Analysis of long non-coding RNA in Cryptosporidium parvum reveals significant stage-specific antisense transcription. Front. Cell Infect. Microbiol. 10, 608298 (2021).
pubmed: 33520737 pmcid: 7840661 doi: 10.3389/fcimb.2020.608298
Tyzzer, E. E. An extracellular Coccidium, Cryptosporidium Muris (Gen. Et Sp. Nov.), of the gastric glands of the common mouse. J. Med. Res. 23, 487–510 (1910).
pubmed: 19971982 pmcid: 2098948
Samuelson, J., Bushkin, G. G., Chatterjee, A. & Robbins, P. W. Strategies to discover the structural components of cyst and oocyst walls. Eukaryot. Cell 12, 1578–1587 (2013).
pubmed: 24096907 pmcid: 3889564 doi: 10.1128/EC.00213-13
Templeton, T. J. et al. The Cryptosporidium oocyst wall protein is a member of a multigene family and has a homolog in Toxoplasma. Infect. Immun. 72, 980–987 (2004).
pubmed: 14742544 pmcid: 321576 doi: 10.1128/IAI.72.2.980-987.2004
Spano, F., Puri, C., Ranucci, L., Putignani, L. & Crisanti, A. Cloning of the entire COWP gene of Cryptosporidium parvum and ultrastructural localization of the protein during sexual parasite development. Parasitology 114, 427–437 (1997).
pubmed: 9149414 doi: 10.1017/S0031182096008761
Katrib, M. et al. Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella. BMC Genomics 13, 685 (2012).
pubmed: 23216867 pmcid: 3770453 doi: 10.1186/1471-2164-13-685
Belli, S. I., Wallach, M. G., Luxford, C., Davies, M. J. & Smith, N. C. Roles of tyrosine-rich precursor glycoproteins and dityrosine- and 3,4-dihydroxyphenylalanine-mediated protein cross-linking in development of the oocyst wall in the coccidian parasite Eimeria maxima. Eukaryot. Cell 2, 456–464 (2003).
pubmed: 12796290 pmcid: 161462 doi: 10.1128/EC.2.3.456-464.2003
Abrahamsen, M. S. et al. Complete genome sequence of the apicomplexan, Cryptosporidium parvum. Science 304, 441–445 (2004).
pubmed: 15044751 doi: 10.1126/science.1094786
Pasquale, S. M. & Goodenough, U. W. Cyclic AMP functions as a primary sexual signal in gametes of Chlamydomonas Reinhardtii. J. Cell Biol. 105, 2279–2292 (1987).
pubmed: 2824527 doi: 10.1083/jcb.105.5.2279
Liu, Y. et al. The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Genes Dev. 22, 1051–1068 (2008).
pubmed: 18367645 pmcid: 2335326 doi: 10.1101/gad.1656508
Snell, W. J. Uncovering an ancestral green menage a trois: contributions of Chlamydomonas to the discovery of a broadly conserved triad of plant fertilization proteins. Curr. Opin. Plant Biol. 69, 102275 (2022).
pubmed: 36007296 pmcid: 9899528 doi: 10.1016/j.pbi.2022.102275
Kafsack, B. F. et al. A transcriptional switch underlies commitment to sexual development in malaria parasites. Nature 507, 248–252 (2014).
pubmed: 24572369 pmcid: 4040541 doi: 10.1038/nature12920
Sinha, A. et al. A cascade of DNA-binding proteins for sexual commitment and development in Plasmodium. Nature 507, 253–257 (2014).
pubmed: 24572359 pmcid: 4105895 doi: 10.1038/nature12970
Russell, A. J. C. et al. Regulators of male and female sexual development are critical for the transmission of a malaria parasite. Cell Host Microbe 31, 305–319 (2023).
pubmed: 36634679 doi: 10.1016/j.chom.2022.12.011
Gomes, A. R. et al. A transcriptional switch controls sex determination in Plasmodium falciparum. Nature 612, 528–533 (2022).
pubmed: 36477538 pmcid: 9750867 doi: 10.1038/s41586-022-05509-z
Oberstaller, J., Joseph, S. J. & Kissinger, J. C. Genome-wide upstream motif analysis of Cryptosporidium parvum genes clustered by expression profile. BMC Genomics 14, 516 (2013).
pubmed: 23895416 pmcid: 3734150 doi: 10.1186/1471-2164-14-516
Brownfield, L. et al. A plant germline-specific integrator of sperm specification and cell cycle progression. PLoS Genet. 5, e1000430 (2009).
pubmed: 19300502 pmcid: 2653642 doi: 10.1371/journal.pgen.1000430
Dubos, C. et al. MYB transcription factors in Arabidopsis. Trends Plant Sci. 15, 573–581 (2010).
pubmed: 20674465 doi: 10.1016/j.tplants.2010.06.005
Waldman, B. S. et al. Identification of a master regulator of differentiation in Toxoplasma. Cell 180, 359–372 (2020).
pubmed: 31955846 pmcid: 6978799 doi: 10.1016/j.cell.2019.12.013
Banaszynski, L. A., Chen, L. C., Maynard-Smith, L. A., Ooi, A. G. & Wandless, T. J. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell 126, 995–1004 (2006).
pubmed: 16959577 pmcid: 3290523 doi: 10.1016/j.cell.2006.07.025
Herm-Götz, A. et al. Rapid control of protein level in the apicomplexan Toxoplasma gondii. Nat. Methods 4, 1003–1005 (2007).
pubmed: 17994029 pmcid: 2601725 doi: 10.1038/nmeth1134
Vinayak, S. et al. Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum. Nature 523, 477–480 (2015).
pubmed: 26176919 pmcid: 4640681 doi: 10.1038/nature14651
Choudhary, H. H., Nava, M. G., Gartlan, B. E., Rose, S. & Vinayak, S. A conditional protein degradation system to study essential gene function in Cryptosporidium parvum. mBio 11, e01231-20 (2020).
pubmed: 32843543 pmcid: 7448269 doi: 10.1128/mBio.01231-20
Xu, R., Beatty, W. L., Greigert, V., Witola, W. H. & Sibley, L. D. Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Nat. Commun. 15, 380 (2024).
pubmed: 38191884 pmcid: 10774378 doi: 10.1038/s41467-024-44696-3
Shaw, S. et al. Genetic crosses within and between species of Cryptosporidium. Proc. Natl Acad. Sci. USA 121, e2313210120 (2024).
pubmed: 38147547 doi: 10.1073/pnas.2313210120
Pawlowic, M. C., Vinayak, S., Sateriale, A., Brooks, C. F. & Striepen, B. Generating and maintaining transgenic Cryptosporidium parvum parasites. Curr. Protoc. Microbiol. 46, 20B.22.21–20B.22.32 (2017).
doi: 10.1002/cpmc.33
Alvarez-Jarreta, J. et al. VEuPathDB: the eukaryotic pathogen, vector and host bioinformatics resource center in 2023. Nucleic Acids Res. 52, D808–D816 (2024).
pubmed: 37953350 doi: 10.1093/nar/gkad1003
Sateriale, A., Pawlowic, M., Vinayak, S., Brooks, C. & Striepen, B. Genetic manipulation of Cryptosporidium parvum with CRISPR/Cas9. Methods Mol. Biol. 2052, 219–228 (2020).
pubmed: 31452165 doi: 10.1007/978-1-4939-9748-0_13
Bray, N. L., Pimentel, H., Melsted, P. & Pachter, L. Near-optimal probabilistic RNA-seq quantification. Nat. Biotechnol. 34, 525–527 (2016).
pubmed: 27043002 doi: 10.1038/nbt.3519
Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26, 139–140 (2010).
pubmed: 19910308 doi: 10.1093/bioinformatics/btp616
Ritchie, M. E. et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47 (2015).
pubmed: 25605792 pmcid: 4402510 doi: 10.1093/nar/gkv007
Zheng, G. X. et al. Massively parallel digital transcriptional profiling of single cells. Nat. Commun. 8, 14049 (2017).
pubmed: 28091601 pmcid: 5241818 doi: 10.1038/ncomms14049
Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177, 1888–1902 (2019).
pubmed: 31178118 pmcid: 6687398 doi: 10.1016/j.cell.2019.05.031
Trapnell, C. et al. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nat. Biotechnol. 32, 381–386 (2014).
pubmed: 24658644 pmcid: 4122333 doi: 10.1038/nbt.2859
Qiu, X. et al. Reversed graph embedding resolves complex single-cell trajectories. Nat. Methods 14, 979–982 (2017).
pubmed: 28825705 pmcid: 5764547 doi: 10.1038/nmeth.4402
Cao, J. et al. The single-cell transcriptional landscape of mammalian organogenesis. Nature 566, 496–502 (2019).
pubmed: 30787437 pmcid: 6434952 doi: 10.1038/s41586-019-0969-x
Baptista, R. P. et al. Long-read assembly and comparative evidence-based reanalysis of Cryptosporidium genome sequences reveal expanded transporter repertoire and duplication of entire chromosome ends including subtelomeric regions. Genome Res. 32, 203–213 (2022).
pubmed: 34764149 pmcid: 8744675 doi: 10.1101/gr.275325.121
Bailey, T. L., Johnson, J., Grant, C. E. & Noble, W. S. The MEME suite. Nucleic Acids Res. 43, W39–W49 (2015).
pubmed: 25953851 pmcid: 4489269 doi: 10.1093/nar/gkv416
Schmittgen, T. D. & Livak, K. J. Analyzing real-time PCR data by the comparative C
pubmed: 18546601 doi: 10.1038/nprot.2008.73
Mauzy, M. J., Enomoto, S., Lancto, C. A., Abrahamsen, M. S. & Rutherford, M. S. The Cryptosporidium parvum transcriptome during in vitro development. PLoS ONE 7, e31715 (2012).
pubmed: 22438867 pmcid: 3305300 doi: 10.1371/journal.pone.0031715

Auteurs

Katelyn A Walzer (KA)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Jayesh Tandel (J)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Jessica H Byerly (JH)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Abigail M Daniels (AM)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Jodi A Gullicksrud (JA)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Eoin C Whelan (EC)

Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Stephen D Carro (SD)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Elise Krespan (E)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Daniel P Beiting (DP)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Boris Striepen (B)

Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA. striepen@upenn.edu.

Classifications MeSH