HIV-1 env gene mutations outside the targeting probe affects IPDA efficiency.

Methodology in biological sciences Virology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
21 Jun 2024
Historique:
received: 08 11 2023
revised: 29 03 2024
accepted: 06 05 2024
medline: 30 5 2024
pubmed: 30 5 2024
entrez: 30 5 2024
Statut: epublish

Résumé

The intact proviral DNA assay (IPDA) based on droplet digital PCR was developed to identify intact proviral DNA and quantify HIV-1 latency reservoirs in patients infected with HIV-1. However, the genetic characteristics of different HIV-1 subtypes are non-consistent due to their high mutation and recombination rates. Here, we identified that the IPDA based on the sequences features of an HIV-1 subtype could not effectively detect different HIV-1 subtypes due to the high diversity of HIV-1. Furthermore, we demonstrated that mutations in

Identifiants

DOI: 10.1016/j.isci.2024.109941 PMID: 38812543 PMC: PMC11133923
pubmed: 38812543
doi: 10.1016/j.isci.2024.109941
pii: S2589-0042(24)01163-5
pmc: PMC11133923
doi:

Types de publication

Journal Article

Langues

eng

Pagination

109941

Informations de copyright

© 2024 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Auteurs

Zehua Zhou (Z)

The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, Sichuan, China.
Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.
Beijing Minhai Biotechnology Co., Ltd., Beijing, China.

Xinyu Zhang (X)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.
College of Life Science, Jilin University, Changchun 130012, China.

Meiyu Wang (M)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.
Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Fei Jiang (F)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.

Jincheng Tong (J)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.

Jianhui Nie (J)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.

Chenyan Zhao (C)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.

Haifa Zheng (H)

Beijing Minhai Biotechnology Co., Ltd., Beijing, China.

Zhen Zhang (Z)

Infection Division, the People's Hospital of Baoding, 608 Dongfeng East Road, Lianchi District, Baoding, Hebei 071000, China.

Penghui Shi (P)

Department of Clinical Laboratory Medicine, the People's Hospital of Baoding, 608 Dongfeng East Road, Lianchi District, Baoding, Hebei 071000, China.

Weiguang Fan (W)

Department of Clinical Laboratory Medicine, the People's Hospital of Baoding, 608 Dongfeng East Road, Lianchi District, Baoding, Hebei 071000, China.

Youchun Wang (Y)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.

Weijin Huang (W)

Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, No. 31 Huatuo Street, Daxing District, Beijing 102629, China.

Classifications MeSH