Tyndallized bacteria prime bronchial epithelial cells to mount an effective innate immune response against infections.
Bronchial epithelial cells
Epithelial homeostasis
Innate immune responses
Tyndallized bacteria
Journal
Human cell
ISSN: 1749-0774
Titre abrégé: Hum Cell
Pays: Japan
ID NLM: 8912329
Informations de publication
Date de publication:
30 May 2024
30 May 2024
Historique:
received:
07
02
2024
accepted:
13
05
2024
medline:
30
5
2024
pubmed:
30
5
2024
entrez:
30
5
2024
Statut:
aheadofprint
Résumé
Airway epithelium represents a physical barrier against toxic substances and pathogens but also presents pattern recognition receptors on the epithelial cells that detect pathogens leading to molecule release and sending signals that activate both the innate and adaptive immune responses. Thus, impaired airway epithelial function and poor integrity may increase the recurrence of infections. Probiotic use in respiratory diseases as adjuvant of traditional therapy is increasingly widespread. There is growing interest in the use of non-viable heat-killed bacteria, such as tyndallized bacteria (TB), due to safety concerns and to their immunomodulatory properties. This study explores in vitro the effects of a TB blend on the immune activation of airway epithelium. 16HBE bronchial epithelial cells were exposed to different concentrations of TB. Cell viability, TB internalization, TLR2 expression, IL-6, IL-8 and TGF-βl expression/release, E-cadherin expression and wound healing were assessed. We found that TB were tolerated, internalized, increased TLR2, E-cadherin expression, IL-6 release and wound healing but decreased both IL-8 and TGF-βl release. In conclusion, TB activate TLR2 pathway without inducing a relevant pro-inflammatory response and improve barrier function, leading to the concept that TB preserve epithelial homeostasis and could be used as strategy to prevent and to manage respiratory infection, exacerbations included.
Identifiants
pubmed: 38814518
doi: 10.1007/s13577-024-01080-z
pii: 10.1007/s13577-024-01080-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Consiglio Nazionale delle Ricerche
ID : Italian National Research Council
Organisme : Consiglio Nazionale delle Ricerche
ID : "One Health Basic
Organisme : Consiglio Nazionale delle Ricerche
ID : Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases (IN-FACT)
Informations de copyright
© 2024. The Author(s).
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