Evaluation of Automated Disk Diffusion Antimicrobial Susceptibility Testing Using Radian® In-Line Carousel.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
30 May 2024
30 May 2024
Historique:
received:
12
12
2023
accepted:
21
04
2024
medline:
31
5
2024
pubmed:
31
5
2024
entrez:
30
5
2024
Statut:
epublish
Résumé
Antimicrobial susceptibility testing (AST) by disk diffusion provides an accurate image of bacterial growth, enabling the detection of culture purity, heterogeneous growth, and antibiotic interactions. However, this manual method is time-consuming and visual interpretation is prone to errors. To overcome these disadvantages, the Radian® In-Line Carousel (Copan, Brescia, Italy) was launched, which is a WASPLab® module dedicated to full automation of (pre)-analytical steps as well as interpretation of disk diffusion AST. However, until now, no evaluation of Radian® against manual disk diffusion has been performed. We assessed the categorical agreement (CA) between standardized disk diffusion (reference method) and Radian® using EUCAST 2021 breakpoints. We tested 135 non-duplicate strains, selected from the National EUCAST challenge panel, clinical strains, and external quality controls. The strains included Enterobacterales (n = 63), Enterococcus faecalis (n = 3), Enterococcus faecium (n = 10), Pseudomonas aeruginosa (n = 16), Staphylococcus aureus (n = 19), coagulase-negative staphylococci (n = 4), and Streptococcus spp. (n = 20). Furthermore, we explored antibiotic disk thermolability in the WASP Radian® carousel by testing 10 ATCC® strains up to 7 days. The observed CA was 95.3%, 96.3%, 93.8%, 97.3% and 98.0% for Enterobacterales, Enterococcus spp., P. aeruginosa, Staphylococcus spp. and Streptococcus spp., respectively, resulting in an acceptable overall CA for all groups. (Very) major error rates were ≤ 5% for all antibiotics. Antibiotic disk thermostability was confirmed up to 4 days in the WASP Radian® In-Line Carousel. The Radian® In-Line Carousel provides a fully automated solution for accurate disk diffusion AST, reducing workload and improving standardization and traceability. In addition, our study demonstrated the thermostability of antibiotic disks up to 4 days in the WASP Radian® In-Line Carousel.
Identifiants
pubmed: 38816509
doi: 10.1007/s00284-024-03710-z
pii: 10.1007/s00284-024-03710-z
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Evaluation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
196Informations de copyright
© 2024. The Author(s).
Références
Banerjee R, Humphries R (2021) Rapid antimicrobial susceptibility testing methods for blood cultures and their clinical impact. Front Med (Lausanne). https://doi.org/10.3389/fmed.2021.635831
doi: 10.3389/fmed.2021.635831
pubmed: 33855033
Herroelen PH, Heestermans R, Emmerechts K, Vandoorslaer K, Wybo I, Piérard D, Muyldermans A (2022) Validation of Rapid Antimicrobial Susceptibility Testing directly from blood cultures using WASPLab®, including Colibrí™ and Radian® In-Line Carousel. Eur J Clin Microbiol Infect Dis 41:733–739
doi: 10.1007/s10096-022-04421-8
pubmed: 35217936
pmcid: 9042988
Cherkaoui A, Renzi G, Vuilleumier N, Schrenzel J (2021) Performance of fully automated antimicrobial disk diffusion susceptibility testing using Copan WASP Colibri coupled to the radian in-line carousel and expert system. J Clin Microbiol. https://doi.org/10.1128/JCM.00777-21
doi: 10.1128/JCM.00777-21
pubmed: 34160274
pmcid: 8373016
Behera B, Anil Vishnu GK, Chatterjee S, Sitaramgupta VVSN, Sreekumar N, Nagabhushan A, Rajendran N, Prathik BH, Pandya HJ (2019) Emerging technologies for antibiotic susceptibility testing. Biosens Bioelectron. https://doi.org/10.1016/j.bios.2019.111552
doi: 10.1016/j.bios.2019.111552
pubmed: 31421358
Khan ZA, Siddiqui MF, Park S (2019) Current and emerging methods of antibiotic susceptibility testing. Diagnostics. https://doi.org/10.3390/diagnostics9020049
doi: 10.3390/diagnostics9020049
pubmed: 31058811
pmcid: 6627445
The European Committe on Antimicrobial Susceptibility testing (2021) Antimicrobial susceptibility testing EUCAST disk diffusion method, Version 9.0
Dauwalder O, Vandenesch F (2020) Disc diffusion AST automation: one of the last pieces missing for full microbiology laboratory automation. Clin Microbiol Infect. https://doi.org/10.1016/j.cmi.2020.01.021
doi: 10.1016/j.cmi.2020.01.021
pubmed: 33276137
Matuschek E, Brown DFJ, Kahlmeter G (2014) Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin Microbiol Infect. https://doi.org/10.1111/1469-0691.12373
doi: 10.1111/1469-0691.12373
pubmed: 24131428
Medeiros AA, Crellin J (2000) Evaluation of the Sirscan automated zone reader in a clinical microbiology laboratory. J Clin Microbiol. https://doi.org/10.1128/JCM.38.4.1688-1693.2000
doi: 10.1128/JCM.38.4.1688-1693.2000
pubmed: 10747172
pmcid: 86529
Zimmermann S (2021) Laboratory automation in the microbiology laboratory: an ongoing journey, not a tale? J Clin Microbiol. https://doi.org/10.1128/JCM.02592-20
doi: 10.1128/JCM.02592-20
pubmed: 33361341
pmcid: 8106703
Cherkaoui A, Renzi G, Vuilleumier N, Schrenzel J (2019) Copan WASPLab automation significantly reduces incubation times and allows earlier culture readings. Clin Microbiol Infect. https://doi.org/10.1016/j.cmi.2019.04.001
doi: 10.1016/j.cmi.2019.04.001
pubmed: 31733376
Cherkaoui A, Renzi G, Viollet A, Fleischmann M, Metral-Boffod L, Dominguez-Amado D, Vuilleumier N, Schrenzel J (2020) Implementation of the WASPLab™ and first year achievements within a university hospital. Eur J Clin Microbiol Infect Dis. https://doi.org/10.1007/s10096-020-03872-1
doi: 10.1007/s10096-020-03872-1
pubmed: 32447536
Desmet S, Verhaegen J, Glupzcynski Y, van Eldere J, Melin P, Goossens H, Piérard D, Declercq P, Lagrou K, Boel A, Cartuyvels R, Denis O, Vandewal W, Saegeman V (2016) Development of a national EUCAST challenge panel for antimicrobial susceptibility testing. Clin Microbiol Infect. https://doi.org/10.1016/j.cmi.2016.05.011
doi: 10.1016/j.cmi.2016.05.011
pubmed: 27234934
The European Committee on Antimicrobial Susceptibility testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0, 2021
Copan WASP AST Module - operator manual HPAWAEN REV04 (2021)
The European committee on Antimicrobial Susceptibility Testing (2021) Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST
The European Committee on Antimicrobial Susceptibility testing. QC tables. Version 11.0, 2021
Cherkaoui A, Renzi G, Fischer A, Azam N, Schorderet D, Vuilleumier N, Schrenzel J (2020) Comparison of the Copan WASPLab incorporating the BioRad expert system against the SIRscan 2000 automatic for routine antimicrobial disc diffusion susceptibility testing. Clin Microbiol Infect 26(5):619–625. https://doi.org/10.1016/j.cmi.2019.11.008
doi: 10.1016/j.cmi.2019.11.008
pubmed: 31733376
Åhman J, Matuschek E, Kahlmeter G (2019) The quality of antimicrobial discs from nine manufacturers-EUCAST evaluations in 2014 and 2017. Clin Microbiol Infect 25(3):346–352
doi: 10.1016/j.cmi.2018.05.021
pubmed: 29886174