Protocol for the cryopreservation of Cryptosporidium isolates using enteroids.

Cell culture Cell isolation Microbiology Organoids

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
01 Jun 2024
Historique:
received: 30 03 2024
revised: 28 04 2024
accepted: 10 05 2024
medline: 2 6 2024
pubmed: 2 6 2024
entrez: 2 6 2024
Statut: aheadofprint

Résumé

A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum-infected HCT-8 cultures. We then detail procedures for the recovery and propagation of frozen parasites using enteroids. For complete details on the use and execution of this protocol, please refer to Deng et al.

Identifiants

DOI: 10.1016/j.xpro.2024.103101 PMID: 38824642
pubmed: 38824642
pii: S2666-1667(24)00266-1
doi: 10.1016/j.xpro.2024.103101
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103101

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Miner Deng (M)

State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Yanting Wei (Y)

State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China.

Na Li (N)

State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Lihua Xiao (L)

State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: lxiao1961@gmail.com.

Yaoyu Feng (Y)

State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: yyfeng@scau.edu.cn.

Yaqiong Guo (Y)

State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: guoyq@scau.edu.cn.

Classifications MeSH