Macrophages treated with interferons induce different responses in lymphocytes via extracellular vesicles.

Cell biology Components of the immune system Immunology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
21 Jun 2024
Historique:
received: 30 11 2023
revised: 09 04 2024
accepted: 08 05 2024
medline: 4 6 2024
pubmed: 4 6 2024
entrez: 4 6 2024
Statut: epublish

Résumé

Limited information exists regarding the impact of interferons (IFNs) on the information carried by extracellular vesicles (EVs). This study aimed at investigating whether IFN-α2b, IFN-β, IFN-γ, and IFN-λ1/2 modulate the content of EVs released by primary monocyte-derived macrophages (MDM). Small-EVs (sEVs) were purified by size exclusion chromatography from supernatants of MDM treated with IFNs. To characterize the concentration and dimensions of vesicles, nanoparticle tracking analysis was used. SEVs surface markers were examined by flow cytometry. IFN treatments induced a significant down-regulation of the exosomal markers CD9, CD63, and CD81 on sEVs, and a significant modulation of some adhesion molecules, major histocompatibility complexes and pro-coagulant proteins, suggesting IFNs influence biogenesis and shape the immunological asset of sEVs. SEVs released by IFN-stimulated MDM also impact lymphocyte function, showing significant modulation of lymphocyte activation and IL-17 release. Altogether, our results show that sEVs composition and activity are affected by IFN treatment of MDM.

Identifiants

pubmed: 38832015
doi: 10.1016/j.isci.2024.109960
pii: S2589-0042(24)01182-9
pmc: PMC11144789
doi:

Types de publication

Journal Article

Langues

eng

Pagination

109960

Informations de copyright

© 2024 The Authors.

Déclaration de conflit d'intérêts

All the authors have no conflicts to declare.

Auteurs

Flavia Giannessi (F)

Neuroimmunology Unit, IRCCS Santa Lucia Foundation, Via Ardeatina 306-354, 00179 Rome, Italy.
Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Zulema Percario (Z)

Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Valentina Lombardi (V)

Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Andrea Sabatini (A)

Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Alessandra Sacchi (A)

Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Veronica Lisi (V)

Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Luca Battistini (L)

Neuroimmunology Unit, IRCCS Santa Lucia Foundation, Via Ardeatina 306-354, 00179 Rome, Italy.

Giovanna Borsellino (G)

Neuroimmunology Unit, IRCCS Santa Lucia Foundation, Via Ardeatina 306-354, 00179 Rome, Italy.

Elisabetta Affabris (E)

Laboratory of Molecular Virology and Antimicrobial Immunity, Department of Science, Roma Tre University, 00146 Rome, Italy.

Daniela F Angelini (DF)

Neuroimmunology Unit, IRCCS Santa Lucia Foundation, Via Ardeatina 306-354, 00179 Rome, Italy.

Classifications MeSH