afila, the origin and nature of a major innovation in the history of pea breeding.
PALMATE‐LIKE PENTAFOLIATA (PALM) genes
afila (af) mutation
breeding history
compound leaf morphology
introgressed haplotypes
legume
megabase‐scale deletion
pea (Pisum sativum L.)
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
05 Jun 2024
05 Jun 2024
Historique:
received:
24
08
2023
accepted:
22
03
2024
medline:
5
6
2024
pubmed:
5
6
2024
entrez:
5
6
2024
Statut:
aheadofprint
Résumé
The afila (af) mutation causes the replacement of leaflets by a branched mass of tendrils in the compound leaves of pea - Pisum sativum L. This mutation was first described in 1953, and several reports of spontaneous af mutations and induced mutants with a similar phenotype exist. Despite widespread introgression into breeding material, the nature of af and the origin of the alleles used remain unknown. Here, we combine comparative genomics with reverse genetic approaches to elucidate the genetic determinants of af. We also investigate haplotype diversity using a set of AfAf and afaf cultivars and breeding lines and molecular markers linked to seven consecutive genes. Our results show that deletion of two tandemly arranged genes encoding Q-type Cys(2)His(2) zinc finger transcription factors, PsPALM1a and PsPALM1b, is responsible for the af phenotype in pea. Eight haplotypes were identified in the af-harbouring genomic region on chromosome 2. These haplotypes differ in the size of the deletion, covering more or less genes. Diversity at the af locus is valuable for crop improvement and sheds light on the history of pea breeding for improved standing ability. The results will be used to understand the function of PsPALM1a/b and to transfer the knowledge for innovation in related crops.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Sixth Framework Programme
ID : FOOD-CT-2004-506223
Organisme : UKRI (BBSRC) for the John Innes Centre Institute Strategic Programme
ID : BBS/E/J/000PR9799
Organisme : Agence Nationale de la Recherche
ID : ANR-09-GENM-026, GENOPEA
Organisme : Agence Nationale de la Recherche
ID : ANR-11-BTBR-0002, PeaMUST
Organisme : EUR Saclay Plant Sciences-SPS
Informations de copyright
© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
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