Clock gene homologs lin-42 and kin-20 regulate circadian rhythms in C. elegans.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 06 2024
05 06 2024
Historique:
received:
07
01
2024
accepted:
15
05
2024
medline:
6
6
2024
pubmed:
6
6
2024
entrez:
5
6
2024
Statut:
epublish
Résumé
Circadian rhythms are endogenous oscillations in nearly all organisms, from prokaryotes to humans, allowing them to adapt to cyclical environments for close to 24 h. Circadian rhythms are regulated by a central clock, based on a transcription-translation feedback loop. One important protein in the central loop in metazoan clocks is PERIOD, which is regulated in part by Casein kinase 1ε/δ (CK1ε/δ) phosphorylation. In the nematode Caenorhabditis elegans, period and casein kinase 1ε/δ are conserved as lin-42 and kin-20, respectively. Here, we studied the involvement of lin-42 and kin-20 in the circadian rhythms of the adult nematode using a bioluminescence-based circadian transcriptional reporter. We show that mutations of lin-42 and kin-20 generate a significantly longer endogenous period, suggesting a role for both genes in the nematode circadian clock, as in other organisms. These phenotypes can be partially rescued by overexpression of either gene under their native promoter. Both proteins are expressed in neurons and epidermal seam cells, as well as in other cells. Depletion of LIN-42 and KIN-20, specifically in neuronal cells after development, was sufficient to lengthen the period of oscillating sur-5 expression. Therefore, we conclude that LIN-42 and KIN-20 are critical regulators of the adult nematode circadian clock through neuronal cells.
Identifiants
pubmed: 38839826
doi: 10.1038/s41598-024-62303-9
pii: 10.1038/s41598-024-62303-9
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
LIN-42 protein, C elegans
0
CLOCK Proteins
EC 2.3.1.48
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12936Subventions
Organisme : NIGMS NIH HHS
ID : R01GM138701
Pays : United States
Organisme : NIH HHS
ID : GM107069
Pays : United States
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : RGPIN-2017-06553
Organisme : Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación
ID : PICT-2021-I-A-01051
Organisme : Universidad Nacional de Quilmes
ID : PUNQ 827- 2285/22
Informations de copyright
© 2024. The Author(s).
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