Low-vacuum SEM imaging and viability test of L929 cells exposed to a Euro 6 diesel exhaust gas mixture in a BAT-CELL chamber in comparison with hydrocarbons emission.
Cell morphology
ESEM
Emission
Gas chromatography
Internal combustion engine
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 06 2024
05 06 2024
Historique:
received:
02
01
2024
accepted:
30
05
2024
medline:
6
6
2024
pubmed:
6
6
2024
entrez:
5
6
2024
Statut:
epublish
Résumé
Exhaust emissions, which count among the most common causes of premature death worldwide, can cause irreversible changes in cells, leading to their damage or degeneration. In this research, L929 line cells were observed after exposure in the BAT-CELL chamber to exhaust gases emitted from a Euro 6 compression-ignition engine. Real road traffic conditions were simulated, taking into account air resistance while driving at speeds of 50 km/h, 120 km/h and idling engine. Morphological analysis of the cells was performed using an environmental scanning electron microscope. It has been observed that diesel exhaust fumes can cause inflammation, which can induce apoptosis or leads to necrotic cell death. The impact of the vehicle exhaust gases can inhibit cell proliferation by almost three times. Moreover, a correlation has been observed between the speed of the inflammatory reaction in cells and the presence of specific hydrocarbon compounds that determine the toxicity of exhaust gases. Research has shown that the toxicity of the emitted exhaust gases has been the highest at the driving speed of 120 km/h. In order to reduce the harmful effects of exhaust emissions, ecological alternatives and the supplementation of legal provisions regarding the compounds subject to limitation are necessary.
Identifiants
pubmed: 38839874
doi: 10.1038/s41598-024-63560-4
pii: 10.1038/s41598-024-63560-4
doi:
Substances chimiques
Vehicle Emissions
0
Hydrocarbons
0
Air Pollutants
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12883Subventions
Organisme : Miniatura grant, National Science Centre, Poland
ID : 2023/07/X/ST10/00090
Informations de copyright
© 2024. The Author(s).
Références
Fundacja Promocji Pojazdów Elektrycznych n.d. https://fppe.pl/ (accessed October 3, 2023).
Bajerlein, M. et al. Modification of a hybrid city bus powertrain in the aspect of lower fuel consumption and exhaust emissions. Appl. Mech. Mater. 518, 108–113. https://doi.org/10.4028/www.scientific.net/AMM.518.108 (2014).
doi: 10.4028/www.scientific.net/AMM.518.108
Ziółkowski, A., Fuć, P., Jagielski, A., Bednarek, M. & Konieczka, S. Comparison of the energy consumption and exhaust emissions between hybrid and conventional vehicles, as well as electric vehicles fitted with a range extender. Energies (Basel) 16, 4669. https://doi.org/10.3390/en16124669 (2023).
doi: 10.3390/en16124669
Landwehr, K. R. et al. Toxicity of different biodiesel exhausts in primary human airway epithelial cells grown at air-liquid interface. Sci. Total Environ. 832, 155016. https://doi.org/10.1016/j.scitotenv.2022.155016 (2022).
doi: 10.1016/j.scitotenv.2022.155016
pubmed: 35381248
Zerboni, A. et al. Diesel exhaust particulate emissions and in vitro toxicity from Euro 3 and Euro 6 vehicles. Environ. Pollut. 297, 118767. https://doi.org/10.1016/j.envpol.2021.118767 (2022).
doi: 10.1016/j.envpol.2021.118767
pubmed: 34974087
Geldenhuys, G., Wattrus, M. & Forbes, P. B. C. Gas and particle phase polycyclic aromatic hydrocarbon emission factors from a diesel vehicle engine: Effect of operating modes in a developing country context. Atmos Environ. X 13, 100158. https://doi.org/10.1016/j.aeaoa.2022.100158 (2022).
doi: 10.1016/j.aeaoa.2022.100158
Das, D. N. & Ravi, N. Influences of polycyclic aromatic hydrocarbon on the epigenome toxicity and its applicability in human health risk assessment. Environ. Res. 213, 113–677. https://doi.org/10.1016/j.envres.2022.113677 (2022).
doi: 10.1016/j.envres.2022.113677
Kęska, A. The actual toxicity of engine exhaust gases emitted from vehicles: The development and perspectives of biological and chemical measurement methods. ACS Omega 8, 24718–24726. https://doi.org/10.1021/acsomega.3c02171 (2023).
doi: 10.1021/acsomega.3c02171
pubmed: 37483244
pmcid: 10357457
Manzetti, S. & Andersen, O. Biochemical and physiological effects from exhaust emissions. A review of the relevant literature. Pathophysiology 23, 285–293. https://doi.org/10.1016/j.pathophys.2016.10.002 (2016).
doi: 10.1016/j.pathophys.2016.10.002
pubmed: 27793419
Sayyed, K. et al. Acute cytotoxicity, genotoxicity, and apoptosis induced by petroleum VOC emissions in A549 cell line. Toxicol. In Vitro 83, 105409. https://doi.org/10.1016/j.tiv.2022.105409 (2022).
doi: 10.1016/j.tiv.2022.105409
pubmed: 35675845
Bisig, C. et al. Assessment of lung cell toxicity of various gasoline engine exhausts using a versatile in vitro exposure system. Environ. Pollut. 235, 263–271. https://doi.org/10.1016/j.envpol.2017.12.061 (2018).
doi: 10.1016/j.envpol.2017.12.061
pubmed: 29291526
Tomašek, I. et al. Respiratory hazard assessment of combined exposure to complete gasoline exhaust and respirable volcanic ash in a multicellular human lung model at the air-liquid interface. Environ. Pollut. 238, 977–987. https://doi.org/10.1016/j.envpol.2018.01.115 (2018).
doi: 10.1016/j.envpol.2018.01.115
pubmed: 29455917
Suarez-Bertoa, R. & Astorga, C. Impact of cold temperature on Euro 6 passenger car emissions. Environ. Pollut. 234, 318–329. https://doi.org/10.1016/j.envpol.2017.10.096 (2018).
doi: 10.1016/j.envpol.2017.10.096
pubmed: 29190540
pmcid: 5817001
Hakkarainen, H. et al. Effects of fuel composition and vehicle operating temperature on in vitro toxicity of exhaust emissions. Environ. Sci. Atmos. https://doi.org/10.1039/D3EA00136A (2024).
doi: 10.1039/D3EA00136A
Mussalo, L. et al. Emissions from modern engines induce distinct effects in human olfactory mucosa cells, depending on fuel and aftertreatment. Sci. Total Environ. 905, 167038. https://doi.org/10.1016/j.scitotenv.2023.167038 (2023).
doi: 10.1016/j.scitotenv.2023.167038
pubmed: 37709087
Kęska, A. et al. Assessment of the actual toxicity of engine exhaust gas emissions from euro 3 and euro 6 compliant vehicles with the BAT-CELL method using in vitro tests. Int J Environ Res Public Health 19, 14138. https://doi.org/10.3390/ijerph192114138 (2022).
doi: 10.3390/ijerph192114138
pubmed: 36361013
pmcid: 9654593
Rozporządzenie Ministra Rodziny, Pracy i Polityki Społecznej z dnia 12 czerwca 2018 r. w sprawie najwyższych dopuszczalnych stężeń i natężeń czynników szkodliwych dla zdrowia w środowisku pracy. n.d.
Nisbet, I. C. T. & LaGoy, P. K. Toxic equivalency factors (TEFs) for polycyclic aromatic hydrocarbons (PAHs). Regulat. Toxicol. Pharmacol. 16, 290–300. https://doi.org/10.1016/0273-2300(92)90009-X (1992).
doi: 10.1016/0273-2300(92)90009-X
Kęska, A. & Janicka, A. Evaluation of toxicity of hydrocarbons emitted by Euro 3 and Euro 6 vehicles at idle conditions by means of equivalent toxicity coefficients. Int. J. Eng. Res. https://doi.org/10.1177/14680874221132929 (2022).
doi: 10.1177/14680874221132929
Stokłosowa S. Hodowla komórek i tkanek. Warszawa: Wydawnictwo Naukowe PWN; 2012.
Abe, S. et al. Characteristic biological effects of itraconazole on L929 fibroblasts and their cell membrane. J. Infect. Chemother. 6, 35–40. https://doi.org/10.1007/s101560050047 (2000).
doi: 10.1007/s101560050047
pubmed: 11810529
Andrych-Zalewska, M., Chlopek, Z., Merkisz, J. & Pielecha, J. Comparison of gasoline engine exhaust emissions of a passenger car through the WLTC and RDE Type Approval Tests. Energies (Basel) 15, 8157. https://doi.org/10.3390/en15218157 (2022).
doi: 10.3390/en15218157
ISO-10993:5, Biologiczna ocena wyrobów medycznych – Część 5: Badania cytotoksyczności in vitro. n.d.
Paduch R. Praktikum z hodowli komórek i tkanek. Lublin: Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej; 2019.
Kęska, A., Janicka, A. & Zawiślak, M. Numerical optimization of the BAT-CELL Bio-Ambient-Tests method for engine exhausts toxicity evaluation. Combustion Engines 192, 19–25. https://doi.org/10.19206/CE-147781 (2023).
doi: 10.19206/CE-147781
Janicka A. Ocena toksyczności mikroatmosfery środowiska wnętrza pojazdu samochodowego. Wrocław: Oficyna Wydawnicza Politechniki Wrocławskiej; 2013.
PN-EN ISO 16017-1:2006, Powietrze wnętrz, atmosferyczne i na stanowiskach pracy. Pobieranie próbek i analiza lotnych związków organicznych z wykorzystaniem rurki sorpcyjnej/desorpcji termicznej/kapilarnej chromatografii gazowej. n.d.
Mendyka B, Syczewska K. Optymalizacja metody przygotowania próbki WWA do analizy chromatograficznej z różnych elementów środowiska, Raport Politechniki Wrocławskiej serii SPR nr 26/99. n.d.
Dopuszczalne normy zanieczyszczeń n.d. https://ekologia.org.pl/index.php/9-co-to-jest-smog/7-dopuszczalne-normy-zanieczyszczen (accessed October 2, 2023).
Kęska A. The problem of the toxic microatmosphere inside vehicle cabins - survey research. W: Nauki Techniczne i Inżynieryjne Cz 4 / [Red Nauk Jacek Leśny, Jędrzej Nyćkowiak] Poznań : Młodzi Naukowcy 2016:63–70.
Benzen n.d. http://archiwum.ciop.pl/11341.html (accessed October 2, 2023).
Emisje i wydajność pojazdów n.d. https://www.eea.europa.eu/pl/pressroom/infografika/emisje-i-wydajnosc-pojazdow/view (accessed October 2, 2023).
Darzynkiewicz, Z., Bedner, E. & Smolewski, P. Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol. 38, 179–193. https://doi.org/10.1016/S0037-1963(01)90051-4 (2001).
doi: 10.1016/S0037-1963(01)90051-4
pubmed: 11309699
Cui, H. et al. Spatial variation and driving mechanism of polycyclic aromatic hydrocarbons (PAHs) emissions from vehicles in China. J. Clean Prod. 336, 130210. https://doi.org/10.1016/j.jclepro.2021.130210 (2022).
doi: 10.1016/j.jclepro.2021.130210
Hussain, M. S. et al. Unlocking the secrets: Volatile Organic Compounds (VOCs) and their devastating effects on lung cancer. Pathol Res Pract 255, 155157. https://doi.org/10.1016/j.prp.2024.155157 (2024).
doi: 10.1016/j.prp.2024.155157
pubmed: 38320440
Naik, G. G. & Dharmadhikari, H. M. Methods for reducing NOx and PM emissions in compression ignition engine: A review. Mater Today Proc 72, 1406–1412. https://doi.org/10.1016/j.matpr.2022.09.339 (2023).
doi: 10.1016/j.matpr.2022.09.339
Nañagas, K. A., Penfound, S. J. & Kao, L. W. Carbon monoxide toxicity. Emerg. Med. Clin. North Am. 40, 283–312. https://doi.org/10.1016/j.emc.2022.01.005 (2022).
doi: 10.1016/j.emc.2022.01.005
pubmed: 35461624