POT1 tumour predisposition: a broader spectrum of associated malignancies and proposal for additional screening program.


Journal

European journal of human genetics : EJHG
ISSN: 1476-5438
Titre abrégé: Eur J Hum Genet
Pays: England
ID NLM: 9302235

Informations de publication

Date de publication:
05 Jun 2024
Historique:
received: 16 12 2023
accepted: 16 04 2024
revised: 03 04 2024
medline: 6 6 2024
pubmed: 6 6 2024
entrez: 5 6 2024
Statut: aheadofprint

Résumé

Protection of Telomeres Protein 1 (POT1) protein is an essential subunit of the shelterin telomere binding complex, regulating telomere length. Some POT1 gene pathogenic variants (PV) lead to telomere elongation, genomic instability and higher risk of cancer. POT1 tumour predisposition syndrome (POT1-TPD) has autosomal dominant inheritance and unknown penetrance. It is associated with increased risk of cutaneous melanoma, chronic lymphocytic leukaemia, angiosarcoma and gliomas. In this work, we aim to describe a broader cancer phenotype related to POT1-TPD, in three families (two with a four generation pedigree, one with a five generation pedigree). The three index cases were referred to our oncogenetic centre for genetic counselling due to their personal history of cancer. Two underwent clinical exome sequencing of 4,867 genes associated with Mendelian genetic diseases, and another underwent gene panel sequencing including POT1, which identified three different POT1 PV: NC_000007.14(NM_015450.2):c.349C>T; NC_000007.14(NM_015450.2):c.233T>C and NC_000007.14(NM_015450.2):c.818G>A; already described in the literature. Referenced relatives, did a target genetic test (according to the POT1 PV identified in the family). In total, 37 individuals were tested (51.4% females), median age of 46 (22-81) years, with POT1 PV detected in 22. POT1-TPD was observed, but also a higher incidence of other cancers (other sarcomas, papillary thyroid cancer, early onset prostate cancer and leukaemia). These findings contribute to an increase in our knowledge about POT1 PV, and it can play a role in the definition of future POT1 PV screening criteria, POT1 carrier surveillance protocols (possibly considering screening for all types of sarcomas) and in genetic counselling.

Identifiants

pubmed: 38839987
doi: 10.1038/s41431-024-01611-0
pii: 10.1038/s41431-024-01611-0
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s).

Références

Henry ML Osborne JR, Else T. POT1 Tumor Predisposition. In: Adam MP, Mirzaa GM, Pagon RA, et al., editors. GeneReviews®. Seattle: University of Washington; 2020.
Wu Y, Poulos RC, Reddel RR. Role of POT1 in Human Cancer. Cancers. 2020;12:2739.
doi: 10.3390/cancers12102739 pubmed: 32987645 pmcid: 7598640
Zade NH, Khattar E. POT1 mutations cause differential effects on telomere length leading to opposing disease phenotypes. J Cell Physiol. 2023;238:1237–55.
doi: 10.1002/jcp.31034 pubmed: 37183325
Vassiliou G. Telomere Length and Clonal Hematopoiesis. N Engl J Med. 2023;388:2481–4.
doi: 10.1056/NEJMe2303022 pubmed: 37140164
Gong Y, Stock AJ, Liu Y. The enigma of excessively long telomeres in cancer: lessons learned from rare human POT1 variants. Curr Opin Genet Dev. 2020;60:48–55.
doi: 10.1016/j.gde.2020.02.002 pubmed: 32155570 pmcid: 7230001
DeBoy EA, Tassia MG, Schratz KE, Yan SM, Cosner ZL, McNally EJ, et al. Familial Clonal Hematopoiesis in a Long Telomere Syndrome. N Engl J Med. 2023;388:2422–33.
doi: 10.1056/NEJMoa2300503 pubmed: 37140166 pmcid: 10501156
Zocchi L, Lontano A, Merli M, Dika E, Nagore E, Quaglino P, et al. Familial Melanoma and Susceptibility Genes: A Review of the Most Common Clinical and Dermoscopic Phenotypic Aspect, Associated Malignancies and Practical Tips for Management. J Clin Med. 2021;10:3760.
doi: 10.3390/jcm10163760 pubmed: 34442055 pmcid: 8397216
Robles-Espinoza CD, Harland M, Ramsay AJ, Aoude LG, Quesada V, Ding Z, et al. POT1 loss-of-function variants predispose to familial melanoma. Nat Genet. 2014;46:478–81.
doi: 10.1038/ng.2947 pubmed: 24686849 pmcid: 4266105
Speedy HE, Kinnersley B, Chubb D, Broderick P, Law PJ, Litchfield K, et al. Germ line mutations in shelterin complex genes are associated with familial chronic lymphocytic leukemia. Blood. 2016;128:2319–26.
doi: 10.1182/blood-2016-01-695692 pubmed: 27528712 pmcid: 5271173
Shen E, Xiu J, Lopez GY, Bentley R, Jalali A, Heimberger AB, et al. POT1 mutation spectrum in tumour types commonly diagnosed among POT1-associated hereditary cancer syndrome families. J Med Genet. 2020;57:664–70.
doi: 10.1136/jmedgenet-2019-106657 pubmed: 31937561
Calvete O, Garcia-Pavia P, Dominguez F, Bougeard G, Kunze K, Braeuninger A, et al. The wide spectrum of POT1 gene variants correlates with multiple cancer types. Eur J Hum Genet. 2017;25:1278–81.
doi: 10.1038/ejhg.2017.134 pubmed: 28853721 pmcid: 5643968
Kim WT, Hennick K, Johnson J, Finnerty B, Choo S, Short SB, et al. Cancer-associated POT1 mutations lead to telomere elongation without induction of a DNA damage response. EMBO J. 2021;40:e107346.
doi: 10.15252/embj.2020107346 pubmed: 33934394 pmcid: 8204863
Herrera-Mullar J, Fulk K, Brannan T, Yussuf A, Polfus L, Richardson ME, et al. Characterization of POT1 tumor predisposition syndrome: Tumor prevalence in a clinically diverse hereditary cancer cohort. Genet Med. 2023;25:100937.
doi: 10.1016/j.gim.2023.100937 pubmed: 37466057
Ishigaki K, Akiyama M, Kanai M, Takahashi A, Kawakami E, Sugishita H, et al. Large-scale genome-wide association study in a Japanese population identifies novel susceptibility loci across different diseases. Nat Genet. 2020;52:669–79.
doi: 10.1038/s41588-020-0640-3 pubmed: 32514122 pmcid: 7968075
Shen S, Li Z, Jiang Y, Duan W, Li H, Du S, et al. A Large-Scale Exome-Wide Association Study Identifies Novel Germline Mutations in Lung Cancer. Am J Respir Crit Care Med. 2023;208:280–9.
doi: 10.1164/rccm.202212-2199OC pubmed: 37167549
Chubb D, Broderick P, Dobbins SE, Frampton M, Kinnersley B, Penegar S, et al. Rare disruptive mutations and their contribution to the heritable risk of colorectal cancer. Nat Commun. 2016;7:11883.
doi: 10.1038/ncomms11883 pubmed: 27329137 pmcid: 4917884
Srivastava A, Miao B, Skopelitou D, Kumar V, Kumar A, Paramasivam N, et al. A Germline Mutation in the POT1 Gene Is a Candidate for Familial Non-Medullary Thyroid Cancer. Cancers. 2020;12:1441.
doi: 10.3390/cancers12061441 pubmed: 32492864 pmcid: 7352431
Mirabello L, Zhu B, Koster R, Karlins E, Dean M, Yeager M, et al. Frequency of Pathogenic Germline Variants in Cancer-Susceptibility Genes in Patients With Osteosarcoma. JAMA Oncol. 2020;6:724–34.
doi: 10.1001/jamaoncol.2020.0197 pubmed: 32191290 pmcid: 7082769
Ballinger ML, Pattnaik S, Mundra PA, Zaheed M, Rath E, Priestley P, et al. Heritable defects in telomere and mitotic function selectively predispose to sarcomas. Science. 2023;379:253–60.
doi: 10.1126/science.abj4784 pubmed: 36656928
Andreotti V, Vanni I, Pastorino L, Ghiorzo P, Bruno W. Germline POT1 Variants: A Critical Perspective on POT1 Tumor Predisposition Syndrome. Genes. 2024;15:104.
doi: 10.3390/genes15010104 pubmed: 38254993 pmcid: 10815363
Zhou FX, Xiong J, Luo ZG, Dai J, Yu HJ, Liao ZK, et al. cDNA expression analysis of a human radiosensitive-radioresistant cell line model identifies telomere function as a hallmark of radioresistance. Radiat Res. 2010;174:550–7.
doi: 10.1667/RR1657.1 pubmed: 20726715
Tang T, Zhou FX, Lei H, Yu HJ, Xie CH, Zhou YF, et al. Increased expression of telomere-related proteins correlates with resistance to radiation in human laryngeal cancer cell lines. Oncol Rep. 2009;21:1505–9.
pubmed: 19424630
Li Q, Wang X, Liu J, Wu L, Xu S. POT1 involved in telomeric DNA damage repair and genomic stability of cervical cancer cells in response to radiation. Mutat Res Genet Toxicol Environ Mutagenesis. 2023;891:503670.
doi: 10.1016/j.mrgentox.2023.503670
Calvete O, Martinez P, Garcia-Pavia P, Benitez-Buelga C, Paumard-Hernandez B, Fernandez V, et al. A mutation in the POT1 gene is responsible for cardiac angiosarcoma in TP53-negative Li-Fraumeni-like families. Nat Commun. 2015;6:8383.
doi: 10.1038/ncomms9383 pubmed: 26403419
Wong K, Robles-Espinoza CD, Rodriguez D, Rudat SS, Puig S, Potrony M, et al. Association of the POT1 Germline Missense Variant p.I78T With Familial Melanoma. JAMA Dermatol. 2019;155:604–9.
doi: 10.1001/jamadermatol.2018.3662 pubmed: 30586141
Potrony M, Puig-Butille JA, Ribera-Sola M, Iyer V, Robles-Espinoza CD, Aguilera P, et al. POT1 germline mutations but not TERT promoter mutations are implicated in melanoma susceptibility in a large cohort of Spanish melanoma families. Br J Dermatol. 2019;181:105–13.
doi: 10.1111/bjd.17443 pubmed: 30451293 pmcid: 6526091
Lim TL, Lieberman DB, Davis AR, Loren AW, Hausler R, Bigdeli A, et al. Germline POT1 variants can predispose to myeloid and lymphoid neoplasms. Leukemia. 2022;36:283–7.
doi: 10.1038/s41375-021-01335-w pubmed: 34193977
Rice C, Shastrula PK, Kossenkov AV, Hills R, Baird DM, Showe LC, et al. Structural and functional analysis of the human POT1-TPP1 telomeric complex. Nat Commun. 2017;8:14928.
doi: 10.1038/ncomms14928 pubmed: 28393830 pmcid: 5394233
Capezzone M, Cantara S, Marchisotta S, Filetti S, De Santi MM, Rossi B, et al. Short telomeres, telomerase reverse transcriptase gene amplification, and increased telomerase activity in the blood of familial papillary thyroid cancer patients. J Clin Endocrinol Metab. 2008;93:3950–7.
doi: 10.1210/jc.2008-0372 pubmed: 18664542
Hakkarainen M, Koski JR, Heckman CA, Anttila P, Silvennoinen R, Lievonen J, et al. A germline exome analysis reveals harmful POT1 variants in multiple myeloma patients and families. EJHaem. 2022;3:1352–7.
doi: 10.1002/jha2.557 pubmed: 36467798 pmcid: 9713058

Auteurs

Marta Baptista Freitas (M)

Centro Hospitalar Universitário de São João, Oporto, Portugal. martacbfreitas@gmail.com.

Laurence Desmyter (L)

Center for Human Genetics, Hôpital Erasme, Hôpital Universitaire de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium.

Cindy Badoer (C)

Center for Human Genetics, Hôpital Erasme, Hôpital Universitaire de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium.

Guillaume Smits (G)

Department of Genetics, Hôpital Universitaire Des Enfants Reine Fabiola, Hôpital Universitaire de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium.

Isabelle Vandernoot (I)

Center for Human Genetics, Hôpital Erasme, Hôpital Universitaire de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium.

Daphné T Kint de Roodenbeke (D)

Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium.

Classifications MeSH