Amniotic fluid stem cell-derived extracellular vesicles educate type 2 conventional dendritic cells to rescue autoimmune disorders in a multiple sclerosis mouse model.
amniotic fluid stem cells
autoimmune diseases
conventional dendritic cell type 2 (cDC2)
dendritic cells
experimental autoimmune encephalomyelitis (EAE)
extracellular vesicles
tolerogenic phenotype
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
Jun 2024
Jun 2024
Historique:
revised:
11
04
2024
received:
24
09
2023
accepted:
15
04
2024
medline:
7
6
2024
pubmed:
7
6
2024
entrez:
6
6
2024
Statut:
ppublish
Résumé
Dendritic cells (DCs) are essential orchestrators of immune responses and represent potential targets for immunomodulation in autoimmune diseases. Human amniotic fluid secretome is abundant in immunoregulatory factors, with extracellular vesicles (EVs) being a significant component. However, the impact of these EVs on dendritic cells subsets remain unexplored. In this study, we investigated the interaction between highly purified dendritic cell subsets and EVs derived from amniotic fluid stem cell lines (HAFSC-EVs). Our results suggest that HAFSC-EVs are preferentially taken up by conventional dendritic cell type 2 (cDC2) through CD29 receptor-mediated internalization, resulting in a tolerogenic DC phenotype characterized by reduced expression and production of pro-inflammatory mediators. Furthermore, treatment of cDC2 cells with HAFSC-EVs in coculture systems resulted in a higher proportion of T cells expressing the regulatory T cell marker Foxp3 compared to vehicle-treated control cells. Moreover, transfer of HAFSC-EV-treated cDC2s into an EAE mouse model resulted in the suppression of autoimmune responses and clinical improvement. These results suggest that HAFSC-EVs may serve as a promising tool for reprogramming inflammatory cDC2s towards a tolerogenic phenotype and for controlling autoimmune responses in the central nervous system, representing a potential platform for the study of the effects of EVs in DC subsets.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12446Subventions
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : AIRC 19903
Organisme : Fondazione Telethon
ID : GMR22T1081
Organisme : PRIN
ID : Prin 2017BZEREZ
Organisme : H2020 European Research Council
ID : ERC
Organisme : H2020 European Research Council
ID : REACT-DC
Organisme : H2020 European Research Council
ID : 101078646
Organisme : Fondazione Italiana Sclerosi Multipla
ID : FISM 2019/R-single/012
Organisme : NextGenerationEU under the Italian MUR Vitality National Innovation Ecosystem grant
ID : ECS00000041
Organisme : University of Perugia Azione di Ateneo 2022 "Regenia" to FF
Informations de copyright
© 2024 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.
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