Design and fabrication of 3D-printed

3D printing crystallization plates external electric fields in situ X-ray crystallography macromolecules

Journal

Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190

Informations de publication

Date de publication:
01 Jun 2024
Historique:
received: 30 12 2023
accepted: 05 03 2024
medline: 7 6 2024
pubmed: 7 6 2024
entrez: 7 6 2024
Statut: epublish

Résumé

X-ray crystallography is an established tool to probe the structure of macromolecules with atomic resolution. Compared with alternative techniques such as single-particle cryo-electron microscopy and micro-electron diffraction, X-ray crystallography is uniquely suited to room-temperature studies and for obtaining a detailed picture of macromolecules subjected to an external electric field (EEF). The impact of an EEF on proteins has been extensively explored through single-crystal X-ray crystallography, which works well with larger high-quality protein crystals. This article introduces a novel design for a 3D-printed

Identifiants

pubmed: 38846773
doi: 10.1107/S1600576724002140
pii: S1600576724002140
pmc: PMC11151662
doi:

Types de publication

Journal Article

Langues

eng

Pagination

842-847

Informations de copyright

© Krishna Prasad Khakurel et al. 2024.

Auteurs

Krishna Prasad Khakurel (KP)

ELI Beamlines Facility, The Extreme Light Infrastructure ERIC, Za Radnicí 835, 25241 Dolní Břežany, Czech Republic.

Michal Nemergut (M)

Center for Interdisciplinary Bio-sciences, Technology and Innovation Park, P. J. Šafárik University, Košice, Slovakia.

Veronika Džupponová (V)

Center for Interdisciplinary Bio-sciences, Technology and Innovation Park, P. J. Šafárik University, Košice, Slovakia.

Kamil Kropielnicki (K)

ELI Beamlines Facility, The Extreme Light Infrastructure ERIC, Za Radnicí 835, 25241 Dolní Břežany, Czech Republic.

Martin Savko (M)

Soleil Synchrotron, Saint-Aubin, France.

Gabriel Žoldák (G)

Center for Interdisciplinary Bio-sciences, Technology and Innovation Park, P. J. Šafárik University, Košice, Slovakia.

Jakob Andreasson (J)

ELI Beamlines Facility, The Extreme Light Infrastructure ERIC, Za Radnicí 835, 25241 Dolní Břežany, Czech Republic.

Classifications MeSH