Depth-enhanced high-throughput microscopy by compact PSF engineering.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
07 Jun 2024
Historique:
received: 24 05 2023
accepted: 03 05 2024
medline: 8 6 2024
pubmed: 8 6 2024
entrez: 7 6 2024
Statut: epublish

Résumé

High-throughput microscopy is vital for screening applications, where three-dimensional (3D) cellular models play a key role. However, due to defocus susceptibility, current 3D high-throughput microscopes require axial scanning, which lowers throughput and increases photobleaching and photodamage. Point spread function (PSF) engineering is an optical method that enables various 3D imaging capabilities, yet it has not been implemented in high-throughput microscopy due to the cumbersome optical extension it typically requires. Here we demonstrate compact PSF engineering in the objective lens, which allows us to enhance the imaging depth of field and, combined with deep learning, recover 3D information using single snapshots. Beyond the applications shown here, this work showcases the usefulness of high-throughput microscopy in obtaining training data for deep learning-based algorithms, applicable to a variety of microscopy modalities.

Identifiants

pubmed: 38849376
doi: 10.1038/s41467-024-48502-y
pii: 10.1038/s41467-024-48502-y
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4861

Subventions

Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 802567
Organisme : Israel Science Foundation (ISF)
ID : 450/18

Informations de copyright

© 2024. The Author(s).

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Auteurs

Nadav Opatovski (N)

Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa, Israel.

Elias Nehme (E)

Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.
Department of Electrical and Computer Engineering, Technion - Israel Institute of Technology, Haifa, Israel.

Noam Zoref (N)

Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.

Ilana Barzilai (I)

Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.

Reut Orange Kedem (R)

Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa, Israel.

Boris Ferdman (B)

Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa, Israel.

Paul Keselman (P)

Sartorius Stedim North America Inc., Bohemia, NY, USA.

Onit Alalouf (O)

Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.

Yoav Shechtman (Y)

Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa, Israel. yoavsh@technion.ac.il.
Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel. yoavsh@technion.ac.il.
Department of Mechanical Engineering, University of Texas at Austin, Austin, TX, USA. yoavsh@technion.ac.il.

Classifications MeSH