Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation.
Journal
Journal of perinatology : official journal of the California Perinatal Association
ISSN: 1476-5543
Titre abrégé: J Perinatol
Pays: United States
ID NLM: 8501884
Informations de publication
Date de publication:
07 Jun 2024
07 Jun 2024
Historique:
received:
14
09
2023
accepted:
02
05
2024
revised:
26
04
2024
medline:
8
6
2024
pubmed:
8
6
2024
entrez:
7
6
2024
Statut:
aheadofprint
Résumé
The objective of this study was to determine the frequency of common gastrointestinal bacterial, parasitic, and viral pathogen detection in necrotizing enterocolitis (NEC) or spontaneous intestinal perforation (SIP) -associated intestinal tissue. Retrospective cohort study examined formalin fixed, paraffin embedded (FFPE) surgical or autopsy intestinal tissue from NEC or SIP specimens. DNA and RNA were extracted and analyzed by multiplex PCR panel (GIFA Biofire). DNA or RNA from stool samples containing each pathogen were extracted for positive controls. The total number of intestinal tissue samples were 193 from 310 infants (156 NEC, 37 SIP). Six (3%) infants with stage III NEC tested positive for a target pathogen; 2, C. difficile; 3, Enteroaggregtive E. coli; and 1, Giardia. No gastrointestinal viral pathogens were detected. Molecular testing yielded few GI pathogens suggesting that these organisms are likely not major causes or facilitators of NEC or SIP.
Identifiants
pubmed: 38849546
doi: 10.1038/s41372-024-01999-z
pii: 10.1038/s41372-024-01999-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Nationwide Children's Hospital (Nationwide Children's)
ID : 20066515
Informations de copyright
© 2024. The Author(s).
Références
Lin PW, Nasr TR, Stoll BJ. Necrotizing enterocolitis: recent scientific advances in pathophysiology and prevention. Semin Perinatol. 2008;32:70–82.
doi: 10.1053/j.semperi.2008.01.004
pubmed: 18346530
Gordon PV, Attridge JT. Understanding clinical literature relevant to spontaneous intestinal perforations. Am J Perinatol. 2009;26:309–16.
doi: 10.1055/s-0028-1103514
pubmed: 19067283
Underwood MA, Mukhopadhyay S, Lakshminrusimha S, Bevins CL Neonatal intestinal dysbiosis. J Perinatol. 2020;40:1597–608
Patel RM, Denning PW. Intestinal microbiota and its relationship with necrotizing enterocolitis. Pediatr Res. 2015;78:232–8.
doi: 10.1038/pr.2015.97
pubmed: 25992911
pmcid: 4655440
Neu J, Walker WA. Necrotizing enterocolitis. N Engl J Med. 2011;364:255–64.
doi: 10.1056/NEJMra1005408
pubmed: 21247316
pmcid: 3628622
Motta C, Scott W, Mahony L, Koch J, Wyckoff M, Reisch J, et al. The association of congenital heart disease with necrotizing enterocolitis in preterm infants: a birth cohort study. J Perinatol. 2015;35:949–53.
doi: 10.1038/jp.2015.96
pubmed: 26248130
Huda S, Chaudhery S, Ibrahim H, Pramanik A. Neonatal necrotizing enterocolitis: clinical challenges, pathophysiology and management. Pathophysiology. 2014;21:3–12.
doi: 10.1016/j.pathophys.2013.11.009
pubmed: 24525171
Vongbhavit K, Underwood MA. Intestinal perforation in the premature infant. J Neonatal Perinat Med. 2017;10:281–9.
doi: 10.3233/NPM-16148
Coggins SA, Wynn JL, Weitkamp JH. Infectious causes of necrotizing enterocolitis. Clin Perinatol. 2015;42:133–54.
doi: 10.1016/j.clp.2014.10.012
pubmed: 25678001
Jilling T, Simon D, Lu J, Meng FJ, Li D, Schy R, et al. The roles of bacteria and TLR4 in rat and murine models of necrotizing enterocolitis. J Immunol. 2006;177:3273–82.
doi: 10.4049/jimmunol.177.5.3273
pubmed: 16920968
Warner BB, Deych E, Zhou Y, Hall-Moore C, Weinstock GM, Sodergren E, et al. Gut bacteria dysbiosis and necrotising enterocolitis in very low birthweight infants: a prospective case-control study. Lancet. 2016;387:1928–36.
doi: 10.1016/S0140-6736(16)00081-7
pubmed: 26969089
pmcid: 5553277
Smith B, Bodé S, Petersen BL, Jensen TK, Pipper C, Kloppenborg J, et al. Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis. BMC Microbiol. 2011;11:73.
doi: 10.1186/1471-2180-11-73
pubmed: 21486476
pmcid: 3094198
Meinzen-Derr J, Morrow AL, Hornung RW, Donovan EF, Dietrich KN, Succop PA. Epidemiology of necrotizing enterocolitis temporal clustering in two neonatology practices. J Pediatr. 2009;154:656–61.
doi: 10.1016/j.jpeds.2008.11.002
pubmed: 19111317
Snyder CL, Hall M, Sharma V, St Peter SD. Seasonal variation in the incidence of necrotizing enterocolitis. Pediatr Surg Int. 2010;26:895–8.
doi: 10.1007/s00383-010-2675-5
pubmed: 20665217
pmcid: 7087736
Sharma R, Garrison RD, Tepas JJ 3rd, Mollitt DL, Pieper P, Hudak ML, et al. Rotavirus-associated necrotizing enterocolitis: an insight into a potentially preventable disease? J Pediatr Surg. 2004;39:453–7.
doi: 10.1016/j.jpedsurg.2003.11.016
pubmed: 15017569
Pumberger W, Novak W. Fatal neonatal Salmonella enteritidis sepsis. J Perinatol. 2000;20:54–6.
doi: 10.1038/sj.jp.7200298
pubmed: 10693101
Han VK, Sayed H, Chance GW, Brabyn DG, Shaheed WA. An outbreak of Clostridium difficile necrotizing enterocolitis: a case for oral vancomycin therapy? Pediatrics. 1983;71:935–41.
doi: 10.1542/peds.71.6.935
pubmed: 6856405
Cushing AH. Necrotizing enterocolitis with Escherichia coli heat-labile enterotoxin. Pediatrics. 1983;71:626–30.
doi: 10.1542/peds.71.4.626
pubmed: 6340045
Rotbart HA, Nelson WL, Glode MP, Triffon TC, Kogut SJ, Yolken RH, et al. Neonatal rotavirus-associated necrotizing enterocolitis: case control study and prospective surveillance during an outbreak. J Pediatr. 1988;112:87–93.
doi: 10.1016/S0022-3476(88)80128-8
pubmed: 3257265
pmcid: 7131392
Butler T, Dahms B, Lindpaintner K, Islam M, Azad MA, Anton P. Segmental necrotising enterocolitis: pathological and clinical features of 22 cases in Bangladesh. Gut. 1987;28:1433–8.
doi: 10.1136/gut.28.11.1433
pubmed: 3428668
pmcid: 1433678
Cichocki M, Singer G, Beyerlein S, Zeder SL, Schober P, Hollwarth M. A case of necrotizing enterocolitis associated with adenovirus infection in a term infant with 22q11 deletion syndrome. J Pediatr Surg. 2008;43:e5–8.
doi: 10.1016/j.jpedsurg.2007.11.034
pubmed: 18405699
Buss SN, Leber A, Chapin K, Fey PD, Bankowski MJ, Jones MK, et al. Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. J Clin Microbiol. 2015;53:915–25.
doi: 10.1128/JCM.02674-14
pubmed: 25588652
pmcid: 4390666
Zhang J, Guan H, Zhao W, Zhang H, Wang W, Ling X, et al. Evaluation of the BioFire FilmArray gastrointestinal panel and real-time polymerase chain reaction assays for the detection of major Diarrheagenic Pathogens by a multicenter Diarrheal Disease Surveillance Program in China. Foodborne Pathog Dis. 2019;16:788–98.
doi: 10.1089/fpd.2019.2642
pubmed: 31478765
Bell MJ, Ternberg JL, Feigin RD, Keating JP, Marshall R, Barton L, et al. Neonatal necrotizing enterocolitis. Therapeutic decisions based upon clinical staging. Ann Surg. 1978;187:1–7.
doi: 10.1097/00000658-197801000-00001
pubmed: 413500
pmcid: 1396409
Panesso-Gómez S, Shimamura M, Conces M, Talavera MM, Moallem M, Sánchez PJ, et al. Detection of cytomegalovirus in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation. J Pediatr. 2019;214:34–40.
doi: 10.1016/j.jpeds.2019.07.038
pubmed: 31493911
Yee WH, Soraisham AS, Shah VS, Aziz K, Yoon W, Lee SK, et al. Incidence and timing of presentation of necrotizing enterocolitis in preterm infants. Pediatrics. 2012;129:e298–304.
doi: 10.1542/peds.2011-2022
pubmed: 22271701
Ullrich T, Tang YW, Correa H, Garzon SA, Maheshwari A, Hill M, et al. Absence of gastrointestinal pathogens in ileum tissue resected for necrotizing enterocolitis. Pediatr Infect Dis J. 2012;31:413–4.
doi: 10.1097/INF.0b013e318242534a
pubmed: 22173144
pmcid: 3305842
Ferraris L, Couturier J, Eckert C, Delannoy J, Barbut F, Butel MJ, et al. Carriage and colonization of C. difficile in preterm neonates: a longitudinal prospective study. PLoS One. 2019;14:e0212568.
doi: 10.1371/journal.pone.0212568
pubmed: 30785934
pmcid: 6382121
Alfa MJ, Robson D, Davi M, Bernard K, Van Caeseele P, Harding GK. An outbreak of necrotizing enterocolitis associated with a novel clostridium species in a neonatal intensive care unit. Clin Infect Dis. 2002;35:S101–105.
doi: 10.1086/341929
pubmed: 12173117
Dittmar E, Beyer P, Fischer D, Schafer V, Schoepe H, Bauer K, et al. Necrotizing enterocolitis of the neonate with Clostridium perfringens: diagnosis, clinical course, and role of alpha toxin. Eur J Pediatr. 2008;167:891–5.
doi: 10.1007/s00431-007-0614-9
pubmed: 17952466
Zhou Y, Shan G, Sodergren E, Weinstock G, Walker WA, Gregory KE. Longitudinal analysis of the premature infant intestinal microbiome prior to necrotizing enterocolitis: a case-control study. PLoS One. 2015;10:e0118632.
doi: 10.1371/journal.pone.0118632
pubmed: 25741698
pmcid: 4351051
Guner YS, Malhotra A, Ford HR, Stein JE, Kelly LK. Association of Escherichia coli O157:H7 with necrotizing enterocolitis in a full-term infant. Pediatr Surg Int. 2009;25:459–63.
doi: 10.1007/s00383-009-2365-3
pubmed: 19396605
Thomas DM, Bell B, Papillon S, Delaplain P, Lim J, Golden J, et al. Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis. PLoS One. 2017;12:e0188211.
doi: 10.1371/journal.pone.0188211
pubmed: 29190745
pmcid: 5708813
Roy SK, Meng Q, Sadowitz BD, Kollisch-Singule M, Yepuri N, Satalin J, et al. Enteral administration of bacteria fermented formula in newborn piglets: a high fidelity model for necrotizing enterocolitis (NEC). PLoS One. 2018;13:e0201172.
doi: 10.1371/journal.pone.0201172
pubmed: 30036384
pmcid: 6056052
George S, Aguilera X, Gallardo P, Farfan M, Lucero Y, Torres JP, et al. Bacterial gut microbiota and infections during early childhood. Front Microbiol. 2021;12:793050.
doi: 10.3389/fmicb.2021.793050
pubmed: 35069488
Ward DV, Scholz M, Zolfo M, Taft DH, Schibler KR, Tett A, et al. Metagenomic sequencing with strain-level resolution implicates Uropathogenic E. coli in necrotizing enterocolitis and mortality in preterm infants. Cell Rep. 2016;14:2912–24.
doi: 10.1016/j.celrep.2016.03.015
pubmed: 26997279
pmcid: 4819403
Beatty JK, Akierman SV, Motta JP, Muise S, Workentine ML, Harrison JJ, et al. Giardia duodenalis induces pathogenic dysbiosis of human intestinal microbiota biofilms. Int J Parasitol. 2017;47:311–26.
doi: 10.1016/j.ijpara.2016.11.010
pubmed: 28237889
Mani S, Hazra S, Hagan J, Sisson A, Nair J, Pammi M. Viral infections and neonatal necrotizing enterocolitis: a meta-analysis. Pediatrics. 2023;152:e2022060876.
Wang F, Flanagan J, Su N, Wang LC, Bui S, Nielson A, et al. RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn. 2012;14:22–9.
doi: 10.1016/j.jmoldx.2011.08.002
pubmed: 22166544
pmcid: 3338343