Interference With VIP to Distinguish Between Real and False VIPoma: National Study From the French Endocrine Tumors Group.
analytic interference
neuroendocrine tumor
vasoactive intestinal peptide
Journal
Journal of the Endocrine Society
ISSN: 2472-1972
Titre abrégé: J Endocr Soc
Pays: United States
ID NLM: 101697997
Informations de publication
Date de publication:
23 May 2024
23 May 2024
Historique:
received:
05
09
2023
medline:
10
6
2024
pubmed:
10
6
2024
entrez:
10
6
2024
Statut:
epublish
Résumé
Vasoactive intestinal peptide (VIP)-secreting tumors (VIPomas) are digestive neuroendocrine tumors in which the hormonal secretion is life-threatening. Biological confirmation is obtained by demonstrating an elevation in plasma VIP, usually using radioimmunoassay (RIA). In some cases, analytical interference is suspected. We developed 3 different techniques to detect interference in VIP RIA. Three techniques were used: RIA after Sephadex column chromatography separation, RIA after polyethylene glycol precipitation, and A total of 15 patients with FPV elevation and 9 RV patients were included. Interference was detected in all FPV patients vs none in RV. Clinical and biochemical parameters did not differ between FPV and RV patients, but VIP concentration in RIA was significantly higher in FPV patients than in RV patients (228 pmol/L vs 66 pmol/L, We developed 3 different laboratory techniques to reveal interference in RIA VIP assays. The diagnostic performance of all 3 was excellent. These techniques must be employed in cases of discordance between VIP elevation and clinical presentation.
Sections du résumé
Background
UNASSIGNED
Vasoactive intestinal peptide (VIP)-secreting tumors (VIPomas) are digestive neuroendocrine tumors in which the hormonal secretion is life-threatening. Biological confirmation is obtained by demonstrating an elevation in plasma VIP, usually using radioimmunoassay (RIA). In some cases, analytical interference is suspected. We developed 3 different techniques to detect interference in VIP RIA.
Methods
UNASSIGNED
Three techniques were used: RIA after Sephadex column chromatography separation, RIA after polyethylene glycol precipitation, and
Results
UNASSIGNED
A total of 15 patients with FPV elevation and 9 RV patients were included. Interference was detected in all FPV patients vs none in RV. Clinical and biochemical parameters did not differ between FPV and RV patients, but VIP concentration in RIA was significantly higher in FPV patients than in RV patients (228 pmol/L vs 66 pmol/L,
Conclusion
UNASSIGNED
We developed 3 different laboratory techniques to reveal interference in RIA VIP assays. The diagnostic performance of all 3 was excellent. These techniques must be employed in cases of discordance between VIP elevation and clinical presentation.
Identifiants
pubmed: 38854908
doi: 10.1210/jendso/bvae102
pii: bvae102
pmc: PMC11156571
doi:
Types de publication
Journal Article
Langues
eng
Pagination
bvae102Informations de copyright
© The Author(s) 2024. Published by Oxford University Press on behalf of the Endocrine Society.