Scriptaid is a prospective agent for improving human asthenozoospermic sample quality and fertilization rate in vitro.
Journal
Asian journal of andrology
ISSN: 1745-7262
Titre abrégé: Asian J Androl
Pays: China
ID NLM: 100942132
Informations de publication
Date de publication:
11 Jun 2024
11 Jun 2024
Historique:
received:
28
09
2023
accepted:
04
03
2024
medline:
10
6
2024
pubmed:
10
6
2024
entrez:
10
6
2024
Statut:
aheadofprint
Résumé
Male infertility is a global issue caused by poor sperm quality, particularly motility. Enhancement of the sperm quality may improve the fertilization rate in assisted reproductive technology (ART) treatment. Scriptaid, with a novel human sperm motility-stimulating activity, has been investigated as a prospective agent for improving sperm quality and fertilization rate in ART. We evaluated the effects of Scriptaid on asthenozoospermic (AZS) semen, including its impact on motility stimulation and protective effects on cryopreservation and duration of motility, by computer-aided sperm analysis (CASA). Sperm quality improvement by Scriptaid was characterized by increased hyaluronan-binding activity, tyrosine phosphorylation, adenosine triphosphate (ATP) concentration, mitochondrial membrane potential, and an ameliorated AZS fertilization rate in clinical intracytoplasmic sperm injection (ICSI) experiments. Furthermore, our identification of active Scriptaid analogs and different metabolites induced by Scriptaid in spermatozoa lays a solid foundation for the future biomechanical exploration of sperm function. In summary, Scriptaid is a potential candidate for the treatment of male infertility in vitro as it improves sperm quality, prolongs sperm viability, and increases the fertilization rate.
Identifiants
pubmed: 38856299
doi: 10.4103/aja202416
pii: 00129336-990000000-00196
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2024 Copyright: ©The Author(s)(2024).
Références
Guzick DS, Overstreet JW, Factor-Litvak P, Brazil CK, Nakajima ST, et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2001;345:1388–93.
Villani MT, Morini D, Spaggiari G, Falbo AI, Melli B, et al. Are sperm parameters able to predict the success of assisted reproductive technology?A retrospective analysis of over 22,000 assisted reproductive technology cycles. Andrology 2022;10:310–21.
Serrano R, Garcia-Marin LJ, Bragado MJ. Sperm phosphoproteome:unraveling male infertility. Biology 2022;11:659.
Cheng YM, Hu XN, Peng Z, Pan TT, Wang F, et al. Lysine glutarylation in human sperm is associated with progressive motility. Hum Reprod 2019;34:1186–94.
Cheng YM, Peng Z, Chen HY, Pan TT, Hu XN, et al. Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility. Hum Reprod 2020;35:494–503.
Sun G, Jiang M, Zhou T, Guo Y, Cui Y, et al. Insights into the lysine acetylproteome of human sperm. J Proteomics 2014;109:199–211.
Yu H, Diao H, Wang C, Lin Y, Yu F, et al. Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Mol Cell Proteom 2015;14:1009–23.
Bhagwat S, Dalvi V, Chandrasekhar D, Matthew T, Acharya K, et al. Acetylated α-tubulin is reduced in individuals with poor sperm motility. Fertil Steril 2014;101:95–104.e3.
Hubbert C, Guardiola A, Shao R, Kawaguchi Y, Ito A, et al. HDAC6 is a microtubule-associated deacetylase. Nature 2002;417:455–8.
Matsuyama A, Shimazu T, Sumida Y, Saito A, Yoshimatsu Y, et al. In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. EMBO J 2002;21:6820–31.
North BJ, Marshall BL, Borra MT, Denu JM, Verdin E. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell 2003;11:437–44.
Palazzo A, Ackerman B, Gundersen GG. Cell biology:tubulin acetylation and cell motility. Nature 2003;421:230.
Parab S, Shetty O, Gaonkar R, Balasinor N, Khole V, et al. HDAC6 deacetylates alpha tubulin in sperm and modulates sperm motility in holtzman rat. Cell Tissue Res 2015;359:665–78.
Yang Y, Ran J, Liu M, Li D, Li Y, et al. CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6. Cell Res 2014;24:1342–53.
Zhang H, Xiao Y, Wang X, Riaz H, Li W, et al. Effects of histone deacetylase inhibitors on the early development of bovine androgenetic embryos. Cell Reprogram 2014;16:54–64.
Kong P, Yin M, Chen D, Li S, Li Y, et al. Effects of the histone deacetylase inhibitor “Scriptaid”on the developmental competence of mouse embryos generated through round spermatid injection. Hum Reprod 2017;32:76–87.
World Health Organization WHO Laboratory Manual for the Examination and Processing of Human Semen 5 th ed Geneva World Health Organization 2010
Szklarczyk D, Morris JH, Cook H, Kuhn M, Wyder S, et al. The STRING database in 2017:quality-controlled protein-protein association networks, made broadly accessible.. Nucleic Acids Res 2017;45:D362–8.
Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, et al. STRING v10:protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res 2015;43:D447–52.
Karnovsky A, Weymouth T, Hull T, Tarcea VG, Scardoni G, et al. Metscape 2 bioinformatics tool for the analysis and visualization of metabolomics and gene expression data. Bioinformatics 2012;28:373–80.
Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, et al. Cytoscape:a software environment for integrated models of biomolecular interaction networks. Genome Res 2003;13:2498–504.
Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril 2004;82:378–83.
Huszar G, Ozenci CC, Cayli S, Zavaczki Z, Hansch E, et al. Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil Steril 2003;79 Suppl 3 1616–24.
Sati L, Cayli S, Delpiano E, Sakkas D, Huszar G. The pattern of tyrosine phosphorylation in human sperm in response to binding to zona pellucida or hyaluronic acid. Reprod Sci 2014;21:573–81.
Xia X, Chen C, Yang L, Wang Y, Duan A, et al. Analysis of metabolites in young and mature Docynia delavayi (Franch.) Schneid leaves using UPLC-ESI-MS/MS. PeerJ 2022;10:e12844.
Huang C, Tang YL, Hu JL, Zhou WJ, Huang ZH, et al. Update on techniques for cryopreservation of human spermatozoa. Asian J Androl 2022;24:563–9.
Yu Z, Wei Z, Yang J, Wang T, Jiang H, et al. Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia:a systematic review and meta-analysis. J Assist Reprod Genet 2018;35:1247–57.
Kang YN, Hsiao YW, Chen CY, Wu CC. Testicular sperm is superior to ejaculated sperm for ICSI in cryptozoospermia:an update systematic review and meta-analysis. Sci Rep 2018;8:7874.
Khoudja R, Li T, Ding C, Xu Y, Liu Y, et al. Effect of co-incubation of oocytes with a decreasing number of spermatozoa on embryo quality. Reprod Biomed Online 2013;26:353–9.
Sakkas D, Ramalingam M, Garrido N, Barratt CL. Sperm selection in natural conception:what can we learn from mother nature to improve assisted reproduction outcomes?. Hum Reprod Update 2015;21:711–26.
Moretti E, Signorini C, Noto D, Corsaro R, Collodel G. The relevance of sperm morphology in male infertility. Front Reprod Health 2022;4:945351.
Vasan SS. Semen analysis and sperm function tests:how much to test?. Indian J Urol 2011;27:41–8.
Sloter E, Schmid TE, Marchetti F, Eskenazi B, Nath J, et al. Quantitative effects of male age on sperm motion. Hum Reprod 2006;21:2868–75.
Nakao S, Takeo T, Watanabe H, Kondoh G, Nakagata N. Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter. Sci Rep 2020;10:8862.
Larsen L, Scheike T, Jensen TK, Bonde JP, Ernst E, et al. Computer-assisted semen analysis parameters as predictors for fertility of men from the general population. The Danish first pregnancy planner study team.. Hum Reprod 2000;15:1562–7.
Li W, Zheng H, Yang Y, Xu H, Guo Z. A diverse English keyword search reveals the value of scriptaid treatment for porcine embryo development following somatic cell nuclear transfer. Reprod Fertil Dev 2022;34:798–803.
Zhang L, Huang Y, Wu Y, Si J, Huang Y, et al. Scriptaid upregulates expression of development-related genes, inhibits apoptosis, and improves the development of somatic cell nuclear transfer mini-pig embryos. Cell Reprogram 2017;19:19–26.
Panda SK, George A, Saha A, Sharma R, Singh AK, et al. Effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of handmade cloned buffalo (Bubalus bubalis) embryos. Theriogenology 2012;77:195–200.
Wang LJ, Zhang H, Wang YS, Xu WB, Xiong XR, et al. Scriptaid improves in vitro development and nuclear reprogramming of somatic cell nuclear transfer bovine embryos. Cell Reprogram 2011;13:431–9.
Sanchez-Rodriguez A, Sansegundo E, Tourmente M, Roldan ER. Effect of high viscosity on energy metabolism and kinematics of spermatozoa from three mouse species incubated under capacitating conditions. Int J Mol Sci 2022;23:15247.
Moustakli E, Zikopoulos A, Sakaloglou P, Bouba I, Sofikitis N, et al. Functional association between telomeres, oxidation and mitochondria. Front Reprod Health 2023;5:1107215.
Paoli D, Gallo M, Rizzo F, Baldi E, Francavilla S, et al. Mitochondrial membrane potential profile and its correlation with increasing sperm motility. Fertil Steril 2011;95:2315–9.
MalićVončina S, Golob B, Ihan A, Kopitar AN, Kolbezen M, et al. Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertil Steril 2016;105:637–44.e1.
Chawan V, Yevate S, Gajbhiye R, Kulkarni V, Parte P. Acetylation/deacetylation and microtubule associated proteins influence flagellar axonemal stability and sperm motility. Biosci Rep 2020;40:BSR20202442.
Leclerc P, de Lamirande E, Gagnon C. Cyclic adenosine 3',5'monophosphate-dependent regulation of protein tyrosine phosphorylation in relation to human sperm capacitation and motility. Biol Reprod 1996;55:684–92.
Jiménez-Trejo F, Tapia-Rodríguez M, Cerbón M, Kuhn DM, Manjarrez-Gutiérrez G, et al. Evidence of 5-HT components in human sperm:implications for protein tyrosine phosphorylation and the physiology of motility. Reproduction 2012;144:677–85.
Zhao W, Li Z, Ping P, Wang G, Yuan X, et al. Outer dense fibers stabilize the axoneme to maintain sperm motility. J Cell Mol Med 2018;22:1755–68.
Bowker Z, Goldstein S, Breitbart H. Protein acetylation protects sperm from spontaneous acrosome reaction. Theriogenology 2022;191:231–8.
Ritagliati C, Luque GM, Stival C, Baro Graf C, Buffone MG, et al. Lysine acetylation modulates mouse sperm capacitation. Sci Rep 2018;8:13334.
Fleming CL, Ashton TD, Nowell C, Devlin M, Natoli A, et al. A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging. Chem Commun 2015;51:7827–30.
Houston B, Curry B, Aitken RJ. Human spermatozoa possess an IL4I1 L-amino acid oxidase with a potential role in sperm function. Reproduction 2015;149:587–96.
Zhu Z, Li R, Feng C, Liu R, Zheng Y, et al. Exogenous oleic acid and palmitic acid improve boar sperm motility via enhancing mitochondrial Β-oxidation for ATP generation. Animals (Basel) 2020;10:591.
Paventi G, Lessard C, Bailey JL, Passarella S. In boar sperm capacitation L-lactate and succinate, but not pyruvate and citrate, contribute to the mitochondrial membrane potential increase as monitored via safranine O fluorescence.. Biochem Biophys Res Commun 2015;462:257–62.
Zhang J, Zhang X, Liu Y, Su Z, Dawar FU, et al. Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway. Oncotarget 2017;8:111807–18.
Kurata S, Hiradate Y, Umezu K, Hara K, Tanemura K. Capacitation of mouse sperm is modulated by gamma-aminobutyric acid (GABA) concentration. J Reprod Dev 2019;65:327–34.
Serafini S, O'Flaherty C. Redox regulation to modulate phosphorylation events in human spermatozoa. Antioxid Redox Signal 2022;37:437–50.