Assessing the efficacy of a novel sperm-washing medium enriched with serotonin, L-carnitine, and coenzyme Q10: an observational cohort study.
Journal
Asian journal of andrology
ISSN: 1745-7262
Titre abrégé: Asian J Androl
Pays: China
ID NLM: 100942132
Informations de publication
Date de publication:
11 Jun 2024
11 Jun 2024
Historique:
received:
25
11
2023
accepted:
14
03
2024
medline:
10
6
2024
pubmed:
10
6
2024
entrez:
10
6
2024
Statut:
aheadofprint
Résumé
This observational cohort study investigated the potential of a novel sperm-washing medium (SWM) enriched with serotonin (5-HT), L-carnitine (L-C), and coenzyme Q10 (CoQ10) to enhance sperm motility and reduce DNA damage. It compared this innovative medium (5-HT/L-C/CoQ10 SWM) with two widely used commercial media (SWM 1 and SWM 2). Ninety-eight volunteers from an infertility clinic provided semen samples, which were divided into three aliquots for analysis in different SWMs: group 1, SWM was composed of hydroxyethyl piperazineethanesulfonic acid (HEPES), sodium bicarbonate, human serum albumin (HSA), taurine, and gentamicin sulfate (SWM 1); group 2, SWM was composed of HEPES, sodium bicarbonate, and HSA (SWM 2); and group 3, SWM was composed of HEPES-buffered human tubal fluid supplemented with 5-HT, L-C, and CoQ10 (5-HT/L-C/CoQ10 SWM). Sperm motility was categorized as progressive, nonprogressive, or immotile. Apoptosis, reactive oxygen species (ROS) production, and DNA fragmentation were also assessed. There were no significant differences in total or progressive sperm motility among the groups. Spermatozoa in group 3 exhibited reduced apoptosis, necrosis, and ROS levels and increased viability. No significant differences were observed in the DNA fragmentation index among groups. The 5-HT/L-C/CoQ10 SWM reduced sperm oxidative stress and apoptosis compared with those of the two commercially available SWMs, suggesting that 5-HT/L-C/CoQ10 SWM could be useful for enhancing in vitro fertilization success rates.
Identifiants
pubmed: 38856308
doi: 10.4103/aja202425
pii: 00129336-990000000-00198
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2024 Copyright: ©The Author(s)(2024).
Références
Eisenberg ML, Esteves SC, Lamb DJ, Hotaling JM, Giwercman A, et al. Male infertility. Nat Rev Dis Primers 2023;9:49.
Chen J, Chen J, Fang Y, Shen Q, Zhao K, et al. Microbiology and immune mechanisms associated with male infertility. Front Immunol 2023;14:1139450.
Mazzilli R, Rucci C, Vaiarelli A, Cimadomo D, Ubaldi FM, et al. Male factor infertility and assisted reproductive technologies:indications, minimum access criteria and outcomes. J Endocrinol Invest 2023;46:1079–85.
Kaltsas A. Oxidative stress and male infertility:the protective role of antioxidants. Medicina (Kaunas) 2023;59:1769.
Vinogradov IV, Gablia MY, Rogozin DS, Tazhetdinov OH, Novikova EG, et al. Treatment of infertility in men with pathospermia associated with oxidative stress. Urologiia 2023;2:73–9.
Hussain T, Kandeel M, Metwally E, Murtaza G, Kalhoro DH, et al. Unraveling the harmful effect of oxidative stress on male fertility:a mechanistic insight. Front Endocrinol (Lausanne) 2023;14:1070692.
Takalani NB, Monageng EM, Mohlala K, Monsees TK, Henkel R, et al. Role of oxidative stress in male infertility. Reprod Fertil 2023;4:e230024.
Chen G, Kathrins M, Ohlander S, Niederberger C. Medical management of male infertility:now and future. Curr Opin Urol 2023;33:10–5.
Lucignani G, Jannello LM, Fulgheri I, Silvani C, Turetti M, et al. Coenzyme Q10 and melatonin for the treatment of male infertility:an arrative review. Nutrients 2022;14:4585.
Khaw SC, Wong ZZ, Anderson R, Martins da Silva S. L-carnitine and l-acetylcarnitine supplementation for idiopathic male infertility. Reprod Fertil 2020;1:67–81.
Pytliak M, Vargová V, Mechírová V, Felšöci M. Serotonin receptors –from molecular biology to clinical applications. Physiol Res 2011;60:15–25.
Aguilar R, Antón F, Bellido C, Aguilar E, Gaytan F. Testicular serotonin is related to mast cells but not to Leydig cells in the rat. J Endocrinol 1995;146:15–21.
Fujinoki M. Serotonin-enhanced hyperactivation of hamster sperm. Reproduction 2011;142:255–66.
Sugiyama Y, Fujinoki M, Shibahara H. Effects of 5-hydroxytryptamine on spermatozoal hyperactivation and in vitro fertilization in mice. J Reprod Dev 2019;65:541–50.
Omote M, Wakimoto Y, Shibahara H. Possible role of 5-hydroxytryptamine (5-HT) receptor on human sperm motility regulation. Cureus 2023;15:e49530.
World Health Organization WHO Laboratory Manual for the Examination and Processing of Human Semen 6 th ed. Geneva World Health Organization 2021
Daru J, Mathur S, Williamson HO. Efficacy of sperm wash media in improving sperm motility. Arch Androl 1988;20:113–5.
Liu K, Mao X, Pan F, Chen Y, An R. Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART. Sci Rep 2023;13:2717.
Sharma P, Kaushal N, Saleth LR, Ghavami S, Dhingra S, et al. Oxidative stress-induced apoptosis and autophagy:balancing the contrary forces in spermatogenesis. Biochim Biophys Acta Mol Basis Dis 2023;1869:166742.
Walke G, Gaurkar SS, Prasad R, Lohakare T, Wanjari M. The impact of oxidative stress on male reproductive function:exploring the role of antioxidant supplementation. Cureus 2023;15:e42583.
Alahmar AT, Calogero AE, Singh R, Cannarella R, Sengupta P, et al. Coenzyme Q10, oxidative stress, and male infertility:a review. Clin Exp Reprod Med 2021;48:97–104.
Liu J, Zhu K, Xu S, Tu W, Lin X, et al. Double-edged sword:effects of human sperm reactive oxygen species on embryo development in IVF cycles. Reprod Biol Endocrinol 2023;21:1.
Chavoshi Nezhad N, Vahabzadeh Z, Allahveisie A, Rahmani K, Raoofi A, et al. The effect of L-carnitine and coenzyme Q10 on the sperm motility, DNA fragmentation, chromatin structure and oxygen free radicals during, before and after freezing in oligospermia men. Urol J 2021;18:330–6.
Amenta F, Vega JA, Ricci A, Collier WL. Localization of 5-hydroxytryptamine-like immunoreactive cells and nerve fibers in the rat female reproductive system. Anat Rec 1992;233:478–84.
Said TM, Gaglani A, Agarwal A. Implication of apoptosis in sperm cryoinjury. Reprod Biomed Online 2010;21:456–62.
Shukla KK, Mahdi AA, Rajender S. Apoptosis, spermatogenesis and male infertility. Front Biosci (Elite Ed) 2012;4:746–54.