Combining Gas Exchange and Rapid Quenching of Leaf Tissue for Mass Spectrometry Analysis Directly in Gas Exchange Cuvette.
13CO2 labeling system
Gas exchange cuvette
Leaf tissue sampling
Mass spectrometry.
Rapid switching
Rapid quenching
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
11
6
2024
pubmed:
11
6
2024
entrez:
11
6
2024
Statut:
ppublish
Résumé
Isotopically nonstationary metabolic flux analysis (INST-MFA) is a powerful technique for studying plant central metabolism, which involves introducing a
Identifiants
pubmed: 38861090
doi: 10.1007/978-1-0716-3802-6_17
doi:
Substances chimiques
Carbon Dioxide
142M471B3J
Carbon Isotopes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
209-219Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Young JD (2014) INCA: a computational platform for isotopically non-stationary metabolic flux analysis. Bioinformatics 30:1333–1335
doi: 10.1093/bioinformatics/btu015
pubmed: 24413674
pmcid: 3998137
Ma F, Jazmin LJ, Young JD, Allen DK (2014) Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc Natl Acad Sci 111:16967–16972
doi: 10.1073/pnas.1319485111
pubmed: 25368168
pmcid: 4250135
Xu Y, Fu X, Sharkey TD, Shachar-Hill Y, Walker BJ (2021) The metabolic origins of non-photorespiratory CO2 release during photosynthesis: a metabolic flux analysis. Plant Physiol 186:297–314. https://doi.org/10.1093/plphys/kiab076
doi: 10.1093/plphys/kiab076
pubmed: 33591309
pmcid: 8154043
Xu Y, Wieloch T, Kaste JAM, Shachar-Hill Y, Sharkey TD (2022) Reimport of carbon from cytosolic and vacuolar sugar pools into the Calvin–Benson cycle explains photosynthesis labeling anomalies. Proc Natl Acad Sci 119:e2121531119
doi: 10.1073/pnas.2121531119
pubmed: 35259011
pmcid: 8931376
Fu X, Gregory LM, Weise SE, Walker BJ (2022) Integrated flux and pool size analysis in plant central metabolism reveals unique roles of glycine and serine during photorespiration. Nat Plants 9:1–10
doi: 10.1038/s41477-022-01294-9
Arrivault S, Guenther M, Ivakov A, Feil R, Vosloh D, Van Dongen JT, Sulpice R, Stitt M (2009) Use of reverse-phase liquid chromatography, linked to tandem mass spectrometry, to profile the Calvin cycle and other metabolic intermediates in Arabidopsis rosettes at different carbon dioxide concentrations. Plant J 59:826–839. https://doi.org/10.1111/j.1365-313X.2009.03902.x
doi: 10.1111/j.1365-313X.2009.03902.x
pubmed: 19453453