descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 Jun 2024
12 Jun 2024
Historique:
received:
06
11
2023
accepted:
24
05
2024
medline:
13
6
2024
pubmed:
13
6
2024
entrez:
12
6
2024
Statut:
epublish
Résumé
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.
Identifiants
pubmed: 38866781
doi: 10.1038/s41467-024-49131-1
pii: 10.1038/s41467-024-49131-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4941Subventions
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP20gm6210027
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21ak0101181
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP22ama221517
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP223fa827004
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP23bm1423012
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21gm1410009
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21cm0106286
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP23ama221220
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP23gm6510018
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP23nk0101665
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP23bm1123032
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP19dm0207078
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21wm0425001
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21zf0127004
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21ak0101181
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22H02824
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22H04926
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP23K20044
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22H02756
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP20H05669
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21K19346
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22KK0100
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22H02523
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP23K18081
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21K20703
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP19K06896
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22K18383
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21H02665
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21K19358
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22K06810
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21H03127
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21H03127
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21K19895
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP20H03549
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP21H05241
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP20H05669
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22H04926
Organisme : MEXT | JST | Core Research for Evolutional Science and Technology (CREST)
ID : JPMJCR23B7
Organisme : MEXT | JST | Core Research for Evolutional Science and Technology (CREST)
ID : JPMJCR20E4
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJTR22UA
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJMS2023
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM104320
Informations de copyright
© 2024. The Author(s).
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