CircZNF609 and circNFIX as possible regulators of glioblastoma pathogenesis via miR-145-5p/EGFR axis.
CircNFIX
CircZNF609
EGFR
miR-145-5p
Glioblastoma
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 06 2024
12 06 2024
Historique:
received:
23
02
2024
accepted:
03
06
2024
medline:
13
6
2024
pubmed:
13
6
2024
entrez:
12
6
2024
Statut:
epublish
Résumé
Glioblastoma is a rare and deadly malignancy with a low survival rate. Emerging evidence has shown that aberrantly expressed circular RNAs (circRNAs) play a critical role in the initiation and progression of GBM tumorigenesis. The oncogenic function of circZNF609 and circNFIX is involved in several types of cancer, but the role and underlying mechanism of these circRNAs in glioblastoma remain unclear. In this study, we hypothesized that circZNF609 and circNFIX may regulate EGFR through sponging miR-145-5p. Herein, we assessed the expression levels of circZNF609, circNFIX, miR-145-5p, and EGFR using quantitative polymerase chain reaction in glioblastoma patients and normal brain samples. The results showed that circZNF609, circNFIX, and EGFR expression levels were upregulated and miR145-5p was downregulated (p = 0.001, 0.06, 0.002, and 0.0065, respectively), while there was no significant association between clinicopathological features of the patients and the level of these genes expression. We also found a significant inverse correlation between miR145-5p and the expression of cZNF609, cNFIX and EGFR (p = 0.0003, 0.0006, and 0.009, respectively). These findings may open a new window for researchers to better understand the potential pathways involved in GBM pathogenesis. In conclusion, it may provide a new potential pathway for the development of effective drugs for the treatment of GBM patients.
Identifiants
pubmed: 38866807
doi: 10.1038/s41598-024-63827-w
pii: 10.1038/s41598-024-63827-w
doi:
Substances chimiques
MicroRNAs
0
MIRN145 microRNA, human
0
ErbB Receptors
EC 2.7.10.1
RNA, Circular
0
EGFR protein, human
EC 2.7.10.1
NFI Transcription Factors
0
NFIX protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
13551Informations de copyright
© 2024. The Author(s).
Références
Louis, D. N. et al. The 2021 WHO classification of tumors of the central nervous system: A summary. Neuro-oncology 23(8), 1231–1251 (2021).
pubmed: 34185076
pmcid: 8328013
doi: 10.1093/neuonc/noab106
Yasinjan, F. et al. Immunotherapy: A promising approach for glioma treatment. Front. Immunol. 2023, 14 (2023).
Li, C. et al. Circular RNA circBFAR promotes glioblastoma progression by regulating a miR-548b/FoxM1 axis. FASEB J. 36(3), e22183 (2022).
pubmed: 35202487
doi: 10.1096/fj.202101307R
Pisignano, G. et al. Going circular: History, present, and future of circRNAs in cancer. Oncogene 42(38), 2783–2800 (2023).
pubmed: 37587333
pmcid: 10504067
doi: 10.1038/s41388-023-02780-w
Wu, M. et al. Circular RNAs: Characteristics, functions, mechanisms, and potential applications in thyroid cancer. Clin. Transl. Oncol. 2023, 1–17 (2023).
Qian, Y. et al. circ-ZNF609: A potent circRNA in human cancers. J. Cell. Mol. Med. 25(22), 10349–10361 (2021).
pubmed: 34697887
pmcid: 8581316
doi: 10.1111/jcmm.16996
Xu, H. et al. NFIX circular RNA promotes glioma progression by regulating miR-34a-5p via notch signaling pathway. Front. Mol. Neurosci. 11, 225 (2018).
pubmed: 30072869
pmcid: 6058096
doi: 10.3389/fnmol.2018.00225
Yin, X. et al. Circular RNA ZNF609 promotes laryngeal squamous cell carcinoma progression by upregulating epidermal growth factor receptor via sponging microRNA-134-5p. Bioengineered 13(3), 6929–6941 (2022).
pubmed: 35236250
pmcid: 8973624
doi: 10.1080/21655979.2022.2034703
Zhang, P. et al. Non-coding RNAs and their integrated networks. Journal of integrative bioinformatics 16(3), 20190027 (2019).
pubmed: 31301674
pmcid: 6798851
doi: 10.1515/jib-2019-0027
Yang, J.-T. et al. ADAM17 confers temozolomide resistance in human glioblastoma cells and miR-145 regulates its expression. Int. J. Mol. Sci. 24(9), 7703 (2023).
pubmed: 37175410
pmcid: 10178422
doi: 10.3390/ijms24097703
Donzelli, S. et al. Epigenetic silencing of miR-145-5p contributes to brain metastasis. Oncotarget 6(34), 35183 (2015).
pubmed: 26440147
pmcid: 4742098
doi: 10.18632/oncotarget.5930
Niu, Y. et al. miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway. Int. J. Biochem. Cell Biolo. 117, 105641 (2019).
doi: 10.1016/j.biocel.2019.105641
Cho, W. C., Chow, A. S. & Au, J. S. MiR-145 inhibits cell proliferation of human lung adenocarcinoma by targeting EGFR and NUDT1. RNA Biol. 8(1), 125–131 (2011).
pubmed: 21289483
doi: 10.4161/rna.8.1.14259
Lu, Y. et al. MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncol. Rep. 29(1), 67–72 (2013).
pubmed: 23076445
doi: 10.3892/or.2012.2084
Sigismund, S., Avanzato, D. & Lanzetti, L. Emerging functions of the EGFR in cancer. Mol. Oncol. 12(1), 3–20 (2018).
pubmed: 29124875
doi: 10.1002/1878-0261.12155
Li, J. et al. Prognostic significance of epidermal growth factor receptor expression in glioma patients. OncoTargets Therapy 2018, 731–742 (2018).
doi: 10.2147/OTT.S155160
Liu, S. et al. FUS-induced circular RNA ZNF609 promotes tumorigenesis and progression via sponging miR-142-3p in lung cancer. J. Cell. Physiol. 236(1), 79–92 (2021).
pubmed: 33459380
doi: 10.1002/jcp.29481
Liu, Z. et al. CircZNF609 promotes cell proliferation, migration, invasion, and glycolysis in nasopharyngeal carcinoma through regulating HRAS via miR-338-3p. Mol. Cell. Biochem. 476, 175–186 (2021).
pubmed: 32970285
doi: 10.1007/s11010-020-03894-5
He, Y. et al. CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions. Cell Death Dis. 11(5), 358 (2020).
pubmed: 32398664
pmcid: 7217914
doi: 10.1038/s41419-020-2441-0
Xiao, E. et al. circNFIX facilitates hepatocellular carcinoma progression by targeting miR-3064-5p/HMGA2 to enhance glutaminolysis. Am. J. Transl. Res. 13(8), 8697 (2021).
pubmed: 34539988
pmcid: 8430108
Dudekula, D. B. et al. CircInteractome: A web tool for exploring circular RNAs and their interacting proteins and microRNAs. RNA Biol. 13(1), 34–42 (2016).
pubmed: 26669964
doi: 10.1080/15476286.2015.1128065
Kim, Y. Z., Kim, C.-Y. & Lim, D. H. The overview of practical guidelines for gliomas by KSNO, NCCN, and EANO. Brain Tumor Res. Treatment 10(2), 83–93 (2022).
doi: 10.14791/btrt.2022.0001
Aldoghachi, A. F. et al. Recent advances in the therapeutic strategies of glioblastoma multiforme. Neuroscience 491, 240–270 (2022).
pubmed: 35395355
doi: 10.1016/j.neuroscience.2022.03.030
Oprita, A. et al. Updated insights on EGFR signaling pathways in glioma. Int. J. Mol. Sci. 22(2), 587 (2021).
pubmed: 33435537
pmcid: 7827907
doi: 10.3390/ijms22020587
Xu, H. et al. Epidermal growth factor receptor in glioblastoma. Oncol. Lett. 14(1), 512–516 (2017).
pubmed: 28693199
pmcid: 5494611
doi: 10.3892/ol.2017.6221
Kadkhoda, S. et al. The importance of regulatory pathway mediated by Circ0001955 in colorectal cancer. Exp. Mol. Pathol. 128, 104819 (2022).
pubmed: 35914612
doi: 10.1016/j.yexmp.2022.104819
Sun, W. et al. Circular RNA: A novel type of biomarker for glioma. Mol. Med. Rep. 24(2), 1–12 (2021).
doi: 10.3892/mmr.2021.12240
Rybak-Wolf, A. et al. Circular RNAs in the mammalian brain are highly abundant, conserved, and dynamically expressed. Mol. Cell 58(5), 870–885 (2015).
pubmed: 25921068
doi: 10.1016/j.molcel.2015.03.027
Liu, Z. et al. Circ-ZNF609 promotes carcinogenesis of gastric cancer cells by inhibiting miRNA-145-5p expression. Eur. Rev. Med. Pharmacol. Sci. 23, 21 (2019).
Yu, X. Z. et al. CircNFIX stimulates the proliferation, invasion, and stemness properties of ovarian cancer cells by enhancing SH3RF3 mRNA stability via binding LIN28B. Kaohsiung J. Med. Sci. 39(3), 234–243 (2023).
pubmed: 36495291
doi: 10.1002/kjm2.12632
Beltran, M., Rossi, F. & Bozzoni, I. CircZNF609 as a prototype to elucidate the biological function of circRNA-mRNA interactions. Mol. Cell. Oncol. 9(1), 2055939 (2022).
pubmed: 35434270
pmcid: 9009943
doi: 10.1080/23723556.2022.2055939
Huang, S. et al. Loss of super-enhancer-regulated circRNA Nfix induces cardiac regeneration after myocardial infarction in adult mice. Circulation 139(25), 2857–2876 (2019).
pubmed: 30947518
pmcid: 6629176
doi: 10.1161/CIRCULATIONAHA.118.038361
Wu, L. et al. Circ-ZNF609 promotes migration of colorectal cancer by inhibiting Gli1 expression via microRNA-150. J. Buon 23(5), 1343–1349 (2018).
pubmed: 30570857
Wu, W. et al. circZNF609 promotes the proliferation and migration of gastric cancer by sponging miR-483-3p and regulating CDK6. OncoTargets Therapy 12, 8197 (2019).
pubmed: 31632070
pmcid: 6783112
doi: 10.2147/OTT.S193031
Wang, S. et al. CircZNF609 promotes breast cancer cell growth, migration, and invasion by elevating p70S6K1 via sponging miR-145-5p. Cancer Manage. Res. 2018, 3881–3890 (2018).
doi: 10.2147/CMAR.S174778
Pan, J. et al. Circ_nuclear factor IX (circNfix) attenuates pressure overload-induced cardiac hypertrophy via regulating miR-145-5p/ATF3 axis. Bioengineered 12(1), 5373–5385 (2021).
pubmed: 34468254
pmcid: 8806771
doi: 10.1080/21655979.2021.1960462
Zhang, Y. et al. Diagnostic and prognostic significance of serum miR-145-5p expression in glioblastoma. Int. J. Clin. Exp. Pathol. 12(7), 2536 (2019).
pubmed: 31934080
pmcid: 6949540
Tong, H. et al. CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell. J. Pharm. Pharmacol. 72(1), 68–75 (2020).
pubmed: 31721211
doi: 10.1111/jphp.13188
Sun, J. et al. Functions and clinical significance of circular RNAs in glioma. Mol. Cancer 19, 1–18 (2020).
doi: 10.1186/s12943-019-1121-0
Long, N. et al. Circular RNA circPOSTN promotes neovascularization by regulating miR-219a-2-3p/STC1 axis and stimulating the secretion of VEGFA in glioblastoma. Cell Death Discov. 8(1), 349 (2022).
pubmed: 35927233
pmcid: 9352789
doi: 10.1038/s41420-022-01136-9
Chen, B. et al. Circular RNA circLGMN facilitates glioblastoma progression by targeting miR-127-3p/LGMN axis. Cancer Lett. 522, 225–237 (2021).
pubmed: 34582975
doi: 10.1016/j.canlet.2021.09.030
Ding, C. et al. CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis. J. Exp. Clin. Cancer Res. 38(1), 1–12 (2019).
doi: 10.1186/s13046-019-1483-6