Three-dimensional chromatin reorganization regulates B cell development during ageing.


Journal

Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575

Informations de publication

Date de publication:
12 Jun 2024
Historique:
received: 01 11 2022
accepted: 16 04 2024
medline: 13 6 2024
pubmed: 13 6 2024
entrez: 12 6 2024
Statut: aheadofprint

Résumé

The contribution of three-dimensional genome organization to physiological ageing is not well known. Here we show that large-scale chromatin reorganization distinguishes young and old bone marrow progenitor (pro-) B cells. These changes result in increased interactions at the compartment level and reduced interactions within topologically associated domains (TADs). The gene encoding Ebf1, a key B cell regulator, switches from compartment A to B with age. Genetically reducing Ebf1 recapitulates some features of old pro-B cells. TADs that are most reduced with age contain genes important for B cell development, including the immunoglobulin heavy chain (Igh) locus. Weaker intra-TAD interactions at Igh correlate with altered variable (V), diversity (D) and joining (J) gene recombination. Our observations implicate three-dimensional chromatin reorganization as a major driver of pro-B cell phenotypes that impair B lymphopoiesis with age.

Identifiants

pubmed: 38866970
doi: 10.1038/s41556-024-01424-9
pii: 10.1038/s41556-024-01424-9
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

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Auteurs

Fei Ma (F)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Yaqiang Cao (Y)

Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, Bethesda, MD, USA.

Hansen Du (H)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Fatima Zohra Braikia (FZ)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Le Zong (L)

Epigenetics and Stem Cell Init, Translational Gerontology Branch, National Institute on Aging, Baltimore, MD, USA.

Noah Ollikainen (N)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Marc Bayer (M)

Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.

Xiang Qiu (X)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Bongsoo Park (B)

Epigenetics and Stem Cell Init, Translational Gerontology Branch, National Institute on Aging, Baltimore, MD, USA.

Roshni Roy (R)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Satabdi Nandi (S)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Dimitra Sarantopoulou (D)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Andrew Ziman (A)

Nikon Instruments Inc., Melville, NY, USA.

Aisha Haley Bianchi (AH)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA.

Isabel Beerman (I)

Epigenetics and Stem Cell Init, Translational Gerontology Branch, National Institute on Aging, Baltimore, MD, USA.

Keji Zhao (K)

Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, Bethesda, MD, USA.

Rudolf Grosschedl (R)

Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.

Ranjan Sen (R)

Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, USA. senranja@grc.nia.nih.gov.

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