Mechanistic insights into G-protein coupling with an agonist-bound G-protein-coupled receptor.


Journal

Nature structural & molecular biology
ISSN: 1545-9985
Titre abrégé: Nat Struct Mol Biol
Pays: United States
ID NLM: 101186374

Informations de publication

Date de publication:
12 Jun 2024
Historique:
received: 09 03 2024
accepted: 14 05 2024
medline: 13 6 2024
pubmed: 13 6 2024
entrez: 12 6 2024
Statut: aheadofprint

Résumé

G-protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by promoting guanine nucleotide exchange. Here, we investigate the coupling of G proteins with GPCRs and describe the events that ultimately lead to the ejection of GDP from its binding pocket in the Gα subunit, the rate-limiting step during G-protein activation. Using molecular dynamics simulations, we investigate the temporal progression of structural rearrangements of GDP-bound G

Identifiants

pubmed: 38867113
doi: 10.1038/s41594-024-01334-2
pii: 10.1038/s41594-024-01334-2
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.

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Auteurs

Hossein Batebi (H)

Universität Leipzig, Medizinische Fakultät, Institut für Medizinische Physik und Biophysik, Leipzig, Germany.
Freie Universität Berlin, Fachbereich Physik, Berlin, Germany.

Guillermo Pérez-Hernández (G)

Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics, Berlin, Germany.

Sabrina N Rahman (SN)

University of Copenhagen, Department of Drug Design and Pharmacology, Copenhagen, Denmark.

Baoliang Lan (B)

State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

Antje Kamprad (A)

Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics, Group Structural Biology of Cellular Signaling, Berlin, Germany.

Mingyu Shi (M)

State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

David Speck (D)

Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics, Group Structural Biology of Cellular Signaling, Berlin, Germany.

Johanna K S Tiemann (JKS)

Universität Leipzig, Medizinische Fakultät, Institut für Medizinische Physik und Biophysik, Leipzig, Germany.
Novozymes A/S, Lyngby, Denmark.

Ramon Guixà-González (R)

Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics, Berlin, Germany.
Department of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain.

Franziska Reinhardt (F)

Universität Leipzig, Department of Computer Science, Bioinformatics, Leipzig, Germany.

Peter F Stadler (PF)

Universität Leipzig, Department of Computer Science, Bioinformatics, Leipzig, Germany.

Makaía M Papasergi-Scott (MM)

Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.

Georgios Skiniotis (G)

Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA.

Patrick Scheerer (P)

Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics, Group Structural Biology of Cellular Signaling, Berlin, Germany.

Brian K Kobilka (BK)

Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.

Jesper M Mathiesen (JM)

University of Copenhagen, Department of Drug Design and Pharmacology, Copenhagen, Denmark.

Xiangyu Liu (X)

State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

Peter W Hildebrand (PW)

Universität Leipzig, Medizinische Fakultät, Institut für Medizinische Physik und Biophysik, Leipzig, Germany. peter.hildebrand@medizin.uni-leipzig.de.
Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics, Berlin, Germany. peter.hildebrand@medizin.uni-leipzig.de.

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