Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation.
Alpha fetoprotein
Dynamic light scattering
Functionalized magnetic nanoparticles
Heptamer
Immunosensor
Nanobody
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
13 Jun 2024
13 Jun 2024
Historique:
received:
20
02
2024
accepted:
13
05
2024
medline:
13
6
2024
pubmed:
13
6
2024
entrez:
13
6
2024
Statut:
epublish
Résumé
A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation. Under the optimized conditions, the designed DLS immunosensor demonstrates excellent linear detection of AFP in the concentration range 0.06 ng mL
Identifiants
pubmed: 38869719
doi: 10.1007/s00604-024-06437-7
pii: 10.1007/s00604-024-06437-7
doi:
Substances chimiques
alpha-Fetoproteins
0
Antibodies, Immobilized
0
Single-Domain Antibodies
0
AFP protein, human
0
Magnetite Nanoparticles
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
387Subventions
Organisme : National Natural Science Foundation of China
ID : 32001788
Organisme : Scientific Research Foundation of Education Department of Anhui Province of China
ID : GJJ200221
Organisme : Jiangxi Provincial Natural Science Foundation
ID : 20224ACB205012
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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