Strand displacement-triggered FRET nanoprobe tracking TK1 mRNA in living cells for ratiometric fluorimetry of nucleic acid biomarker.
Fluorescence resonance energy transfer
Ratiometric fluorescent probe
Strand displacement
Thymidine kinase 1
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
13 Jun 2024
13 Jun 2024
Historique:
received:
31
01
2024
accepted:
20
05
2024
medline:
14
6
2024
pubmed:
14
6
2024
entrez:
13
6
2024
Statut:
epublish
Résumé
A precisely designed dual-color biosensor has realized a visual assessment of thymidine kinase 1 (TK1) mRNA in both living cells and cell lysates. The oligonucleotide probe is constructed by hybridizing the antisense strand of the target and two recognition sequences, in which FAM serves as the donor and TAMRA as the acceptor. Once interacting with the target, two recognition strands are replaced, and then the antisense complementary sequence forms a more stable double-stranded structure. Due to the increasing spatial distance between two dyes, the FRET is attenuated, leading to a rapid recovery of FAM fluorescence and a reduction of TAMRA fluorescence. A discernible color response from orange to green could be observed by the naked eye, with a limit of detection (LOD) of 0.38 nM and 5.22 nM for spectrometer- and smartphone-based assays, respectively. The proposed ratiometric method transcends previous reports in its capacities in visualizing TK1 expression toward reliable nucleic acid biomarker analysis, which might establish a general strategy for ratiometric biosensing via strand displacement.
Identifiants
pubmed: 38871953
doi: 10.1007/s00604-024-06453-7
pii: 10.1007/s00604-024-06453-7
doi:
Substances chimiques
Thymidine Kinase
EC 2.7.1.21
thymidine kinase 1
EC 2.7.1.21
RNA, Messenger
0
Fluorescent Dyes
0
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
390Subventions
Organisme : National Natural Science Foundation of China
ID : 21703255
Organisme : Anhui Province Science Fund for Outstanding Young Scholars
ID : 2208085Y27
Organisme : Scientific Research Foundation for the Returned Overseas Chinese Scholars of Anhui Province-Key Projects
ID : 2021LCX003
Organisme : Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province
ID : gxyqZD2021102
Organisme : Research Fund of Anhui Institute of Translational Medicine
ID : 2021zhyx-C17
Organisme : Research Fund for Scientific Research Level Improvement Plan of Anhui Medical University
ID : 2020xkjT001
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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