Improved breast milk proteome coverage by DIA based LC-MS/MS method.
breast milk
colostrum
mass spectrometry
obesity
proteomics
Journal
Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707
Informations de publication
Date de publication:
14 Jun 2024
14 Jun 2024
Historique:
revised:
18
05
2024
received:
10
09
2023
accepted:
22
05
2024
medline:
14
6
2024
pubmed:
14
6
2024
entrez:
14
6
2024
Statut:
aheadofprint
Résumé
The breast milk composition includes a multitude of bioactive factors such as viable cells, lipids and proteins. Measuring the levels of specific proteins in breast milk plasma can be challenging because of the large dynamic range of protein concentrations and the presence of interfering substances. Therefore, most proteomic studies of breast milk have been able to identify under 1000 proteins. Optimised procedures and the latest separation technologies used in milk proteome research could lead to more precise knowledge of breast milk proteome. This study (n = 53) utilizes three different protein quantification methods, including direct DIA, library-based DIA method and a hybrid method combining direct DIA and library-based DIA. On average we identified 2400 proteins by hybrid method. By applying these methods, we quantified body mass index (BMI) associated variation in breast milk proteomes. There were 210 significantly different proteins when comparing the breast milk proteome of obese and overweight mothers. In addition, we analysed a small cohort (n = 5, randomly selected from 53 samples) by high field asymmetric waveform ion mobility spectrometry (FAIMS). FAIMS coupled with the Orbitrap Fusion Lumos mass spectrometer, which led to 41.7% higher number of protein identifications compared to Q Exactive HF mass spectrometer.
Identifiants
pubmed: 38873899
doi: 10.1002/pmic.202300340
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300340Subventions
Organisme : Research Council of Finland
ID : 258606
Organisme : Research Council of Finland
ID : 332582
Organisme : Diabetes Research Foundation
Organisme : Päivikki and Sakari Sohlberg Foundation
Organisme : Sigrid Juselius Foundation
Organisme : Juho Vainion Foundation
Organisme : the State Research Funding for university-level health research in the Turku University Hospital Expert Responsibility Area
Organisme : Tyks Foundation
Informations de copyright
© 2024 The Author(s). Proteomics published by Wiley‐VCH GmbH.
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