An Exo III-powered closed-loop DNA circuit architecture for biosensing/imaging.
Fluorescence imaging
AND logic gate
Biosensing
Cell imaging
DNA circuit
Exonuclease III (Exo III)
miR-21
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
14 Jun 2024
14 Jun 2024
Historique:
received:
25
02
2024
accepted:
24
05
2024
medline:
15
6
2024
pubmed:
15
6
2024
entrez:
14
6
2024
Statut:
epublish
Résumé
With their regulated Boolean logic operations in vitro and in vivo, DNA logic circuits have shown great promise for target recognition and disease diagnosis. However, significant obstacles must be overcome to improve their operational efficiency and broaden their range of applications. In this study, we propose an Exo III-powered closed-loop DNA circuit (ECDC) architecture that integrates four highly efficient AND logic gates. The ECDC utilizes Exo III as the sole enzyme-activated actuator, simplifying the circuit design and ensuring optimal performance. Moreover, the use of Exo III enables a self-feedback (autocatalytic) mechanism in the dynamic switching between AND logic gates within this circulating logic circuit. After validating the signal flow and examining the impact of each AND logic gate on the regulation of the circuit, we demonstrate the intelligent determination of miR-21 using the carefully designed ECDC architecture in vitro. The proposed ECDC exhibits a linear detection range for miR-21 from 0 to 300 nM, with a limit of detection (LOD) of approximately 0.01 nM, surpassing most reported methods. It also shows excellent selectivity for miR-21 detection and holds potential for identifying and imaging live cancer cells. This study presents a practical and efficient strategy for monitoring various nucleic acid-based biomarkers in vitro and in vivo through specific sequence modifications, offering significant potential for early cancer diagnosis, bioanalysis, and prognostic clinical applications.
Identifiants
pubmed: 38877347
doi: 10.1007/s00604-024-06476-0
pii: 10.1007/s00604-024-06476-0
doi:
Substances chimiques
exodeoxyribonuclease III
EC 3.1.11.2
MicroRNAs
0
Exodeoxyribonucleases
EC 3.1.-
MIRN21 microRNA, human
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
395Subventions
Organisme : Open Fund from Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, China
ID : No. CELLPHYSIOL/SXMU-2021-13
Organisme : Shanxi Provincial Health Commission scientific research project
ID : No.2023058
Organisme : General Program of Applied Basic Research Plan of Shanxi Province
ID : No.202103021224088
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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