Multifaceted cancer alleviation by cowpea mosaic virus in a bioprinted ovarian cancer peritoneal spheroid model.

3D bioprinting Cancer immunotherapy Cowpea mosaic virus In vitro models Ovarian cancer

Journal

Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316

Informations de publication

Date de publication:
10 Jun 2024
Historique:
received: 29 01 2024
revised: 01 06 2024
accepted: 08 06 2024
medline: 16 6 2024
pubmed: 16 6 2024
entrez: 15 6 2024
Statut: aheadofprint

Résumé

Ovarian cancer (OvCa) is a leading cause of mortality among gynecological malignancies and usually manifests as intraperitoneal spheroids that generate metastases, ascites, and an immunosuppressive tumor microenvironment. In this study, we explore the immunomodulatory properties of cowpea mosaic virus (CPMV) as an adjuvant immunotherapeutic agent using an in vitro model of OvCa peritoneal spheroids. Previous findings highlighted the potent efficacy of intratumoral CPMV against OvCa in mouse tumor models. Leveraging the precision control over material deposition and cell patterning afforded by digital-light-processing (DLP) based bioprinting, we constructed OvCa-macrophage spheroids to mimic peritoneal spheroids using gelatin methacrylate (GelMA), a collagen-derived photopolymerizable biomaterial to mimic the extracellular matrix. Following CPMV treatment, bioprinted spheroids exhibited inhibited OvCa progression mediated by macrophage activation. Our analysis indicates that CPMV regulates and activates macrophage to both induce OvCa cell killing and restore normal cell-cell junctions. This study deepened our understanding of the mechanism of CPMV intratumoral immunotherapy in the setting of OvCa. This study also highlights the potential of studying immunotherapies using high throughput tissue models via DLP bioprinting.

Identifiants

DOI: 10.1016/j.biomaterials.2024.122663 PMID: 38878481
pubmed: 38878481
pii: S0142-9612(24)00197-2
doi: 10.1016/j.biomaterials.2024.122663
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122663

Informations de copyright

Copyright © 2024 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest Nicole F. Steinmetz reports a relationship with Mosaic ImmunoEnginering Inc. that includes: equity or stocks. Steven N. Fiering reports a relationship with Mosaic ImmunoEnginering Inc. that includes: equity or stocks. Nicole F. Steinmetz reports a relationship with Pokometz Scientific LLC that includes: consulting or advisory. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Yi Xiang (Y)

Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA.

Zhongchao Zhao (Z)

Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Center for Nano-ImmunoEngineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Moores Cancer Center, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA.

Emmie J Yao (EJ)

Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA.

Alis Balayan (A)

Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; School of Medicine, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA.

Steven N Fiering (SN)

Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, 03756, USA; Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth and Dartmouth Health, Lebanon, NH, 03756, USA.

Nicole F Steinmetz (NF)

Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Center for Nano-ImmunoEngineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Moores Cancer Center, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Department of Radiology, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Institute for Materials Discovery and Design, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Center for Engineering in Cancer, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Shu and K.C. Chien and Peter Farrell Collaboratory, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA. Electronic address: nsteinmetz@ucsd.edu.

Shaochen Chen (S)

Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Center for Nano-ImmunoEngineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA; Institute for Materials Discovery and Design, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA. Electronic address: shc064@ucsd.edu.

Classifications MeSH