Improved glucose detection limit based on phosphorescence from protected metalloporphyrin triplet state.
Glucose detection
Improved detection limit
Phosphorescence emission
Triplet protector
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
01 Aug 2024
01 Aug 2024
Historique:
received:
25
12
2023
revised:
25
04
2024
accepted:
04
06
2024
medline:
16
6
2024
pubmed:
16
6
2024
entrez:
15
6
2024
Statut:
ppublish
Résumé
Non-invasive indirect blood glucose monitoring can be realized by detecting low concentrations of glucose (0.05-5 mM) in tears, but sensitive optical indicators are required. The intensity of the phosphorescence of a candidate optical indicator, palladium hematoporphyrin monomethyl ether (Pd-HMME), is increased by oxygen consumption under sealed conditions in the presence of glucose and glucose oxidase. However, the glucose detection limit based on this mechanism is high (800 μM) because the phosphorescence is completely quenched under ambient oxygen conditions and hence a large amount of glucose is required to reduce the oxygen levels such that the phosphorescence signal is detectable. To improve the glucose detection limit of Pd-HMME phosphorescence-based methods, the triplet protector imidazole was introduced, and strong phosphorescence was observed under ambient oxygen conditions. Detectable phosphorescence enhancement occurred at low glucose concentrations (<200 μM). Linear correlation between the phosphorescence intensity and glucose concentration was observed in the range of 30-727 μM (R These results indicate the potential of the developed optical indicator for fast, selective, and reliable low-concentration glucose sensing.
Sections du résumé
BACKGROUND
BACKGROUND
Non-invasive indirect blood glucose monitoring can be realized by detecting low concentrations of glucose (0.05-5 mM) in tears, but sensitive optical indicators are required. The intensity of the phosphorescence of a candidate optical indicator, palladium hematoporphyrin monomethyl ether (Pd-HMME), is increased by oxygen consumption under sealed conditions in the presence of glucose and glucose oxidase. However, the glucose detection limit based on this mechanism is high (800 μM) because the phosphorescence is completely quenched under ambient oxygen conditions and hence a large amount of glucose is required to reduce the oxygen levels such that the phosphorescence signal is detectable.
RESULTS
RESULTS
To improve the glucose detection limit of Pd-HMME phosphorescence-based methods, the triplet protector imidazole was introduced, and strong phosphorescence was observed under ambient oxygen conditions. Detectable phosphorescence enhancement occurred at low glucose concentrations (<200 μM). Linear correlation between the phosphorescence intensity and glucose concentration was observed in the range of 30-727 μM (R
SIGNIFICANCE AND NOVELTY
UNASSIGNED
These results indicate the potential of the developed optical indicator for fast, selective, and reliable low-concentration glucose sensing.
Identifiants
pubmed: 38879210
pii: S0003-2670(24)00626-3
doi: 10.1016/j.aca.2024.342825
pii:
doi:
Substances chimiques
Hematoporphyrins
0
Palladium
5TWQ1V240M
Glucose
IY9XDZ35W2
Glucose Oxidase
EC 1.1.3.4
hematoporphyrin monomethyl ether
0
Blood Glucose
0
Imidazoles
0
imidazole
7GBN705NH1
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
342825Informations de copyright
Copyright © 2024. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.