Robust, fully quantifiable and scalable bioprocess utilizing spent sulfite liquor with Corynebacterium glutamicum.

fermentation fluorescence biomass measurement lignocellulosic feedstock minimal medium scale-up

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
14 Jun 2024
Historique:
received: 25 04 2024
revised: 02 06 2024
accepted: 11 06 2024
medline: 17 6 2024
pubmed: 17 6 2024
entrez: 16 6 2024
Statut: aheadofprint

Résumé

In this study, a bioprocessing strategy was designed to valorize ultra-filtered spent sulfite liquor (UF-SSL) without prior detoxification steps as well as using it purely as a carbon source supplement to defined or complex media. Hence, a minimal medium for the bioconversion of UF-SSL with Corynebacterium glutamicum was developed and process robustness and reproducibility were validated. Process quantifiability was ensured by development of a biomass measurement technique for matrices with high water-insoluble solids and verified using elemental balancing. Mechanistic modeling based on Monod equations was used to identify batch kinetics. In a final step, scale-up of the developed process was performed to showcase process maturity towards commercialisation.

Identifiants

DOI: 10.1016/j.biortech.2024.130967 PMID: 38880268
pubmed: 38880268
pii: S0960-8524(24)00671-0
doi: 10.1016/j.biortech.2024.130967
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

130967

Informations de copyright

Copyright © 2024. Published by Elsevier Ltd.

Auteurs

Daniel Waldschitz (D)

Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria.

Mark-Richard Neudert (MR)

Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria.

Jakob Kitzmueller (J)

Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria.

Johanna Lachmann (J)

Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria.

Arthur Fonteyne (A)

Bio Base Europe Pilot Plant vzw, Rodenhuizekaai 1, Gent, B-9042, Belgium.

Karolien Maes (K)

Bio Base Europe Pilot Plant vzw, Rodenhuizekaai 1, Gent, B-9042, Belgium.

Nadav Bar (N)

Department of Chemical Engineering, Faculty of Natural Sciences, NTNU, Sem Sælands vei 6, Trondheim, N-7491, Norway.

Peter Sinner (P)

Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria.

Oliver Spadiut (O)

Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria. Electronic address: oliver.spadiut@tuwien.ac.at.

Classifications MeSH