A synchronized, large-scale field experiment using Arabidopsis thaliana reveals the significance of the UV-B photoreceptor UVR8 under natural conditions.
flavonoid
glucosinolate
metabolite
plant morphology
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
16 Jun 2024
16 Jun 2024
Historique:
revised:
03
06
2024
received:
05
04
2024
accepted:
06
06
2024
medline:
17
6
2024
pubmed:
17
6
2024
entrez:
17
6
2024
Statut:
aheadofprint
Résumé
This study determines the functional role of the plant ultraviolet-B radiation (UV-B) photoreceptor, UV RESISTANCE LOCUS 8 (UVR8) under natural conditions using a large-scale 'synchronized-genetic-perturbation-field-experiment'. Laboratory experiments have demonstrated a role for UVR8 in UV-B responses but do not reflect the complexity of outdoor conditions where 'genotype × environment' interactions can mask laboratory-observed responses. Arabidopsis thaliana knockout mutant, uvr8-7, and the corresponding Wassilewskija wild type, were sown outdoors on the same date at 21 locations across Europe, ranging from 39°N to 67°N latitude. Growth and climatic data were monitored until bolting. At the onset of bolting, rosette size, dry weight, and phenolics and glucosinolates were quantified. The uvr8-7 mutant developed a larger rosette and contained less kaempferol glycosides, quercetin glycosides and hydroxycinnamic acid derivatives than the wild type across all locations, demonstrating a role for UVR8 under field conditions. UV effects on rosette size and kaempferol glycoside content were UVR8 dependent, but independent of latitude. In contrast, differences between wild type and uvr8-7 in total quercetin glycosides, and the quercetin-to-kaempferol ratio decreased with increasing latitude, that is, a more variable UV response. Thus, the large-scale synchronized approach applied demonstrates a location-dependent functional role of UVR8 under natural conditions.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Higher Education, Science and Innovation, Republic of Slovenia
ID : P1-0212
Organisme : The Carl Trygger Foundation for Scientific Research, Sweden
ID : #CTS21:1666
Organisme : Knowledge Foundation, Sweden
ID : #20130164
Organisme : Swedish Research Council Formas, Sweden
ID : #942-2015-516
Organisme : Faculty for Business, Science and Technology at Örebro University
Organisme : Hungarian Scientific Research Fund
ID : OTKA
Organisme : Hungarian Scientific Research Fund
ID : K-138022
Organisme : Hungarian Scientific Research Fund
ID : K-132633
Organisme : State Investigation Agency, Spain
ID : AGL2010-22196-C02-01
Organisme : State Investigation Agency, Spain
ID : FPI BES-2011-045885
Organisme : Spanish Government
ID : CGL2014-55976-R
Organisme : Government of La Rioja
ID : "Afianza" 2023/05
Organisme : Science Foundation Ireland
ID : 16-IA-4418
Pays : Ireland
Organisme : Science Foundation Ireland
ID : F3707
Pays : Ireland
Organisme : Science Foundation Ireland
ID : 267360
Pays : Ireland
Organisme : Science Foundation Ireland
ID : UV4growth
Pays : Ireland
Organisme : Austrian Science Fund (FWF)
Organisme : Academy of Finland
Organisme : COST action FA0906
Informations de copyright
© 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.
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