Oral Papillary Squamous Cell Carcinoma and Oral Squamous Cell Carcinoma: A Histopathological and Immunohistochemical Comparative Study.
Alpha smooth muscle actin
Moderately differentiated squamous cell carcinoma
Oral papillary squamous cell carcinoma
Poorly differentiated squamous cell carcinoma
Transforming growth factor beta
Well-differentiated squamous cell carcinoma
Journal
Head and neck pathology
ISSN: 1936-0568
Titre abrégé: Head Neck Pathol
Pays: United States
ID NLM: 101304010
Informations de publication
Date de publication:
17 Jun 2024
17 Jun 2024
Historique:
received:
28
12
2023
accepted:
26
02
2024
medline:
17
6
2024
pubmed:
17
6
2024
entrez:
17
6
2024
Statut:
epublish
Résumé
The aim of the study is to investigate the immunohistochemical expression of both Alpha smooth muscle actin and Transforming Growth Factor beta and compare their expression in oral papillary squamous cell carcinoma with their expression in different histological grades of oral squamous cell carcinoma. A correlation between these immuno-histochemical expressions and histological findings will then be performed. The research question is "Do the percentages of α-SMA and TGF-β immune-expression in OPSCC differ from that in the conventional OSCC?". This will be achieved by collecting archival blocks of oral papillary squamous cell carcinoma and different grades of oral squamous cell carcinoma, staining the specimens with Transforming Growth Factor beta and alpha smooth muscle actin, then measuring the mean staining index of expression in each group and the area percent of both markers. Results revealed that transforming growth factor beta expression in the epithelium was high in all cases of well-differentiated squamous cell carcinoma, most oral papillary squamous cell carcinoma, and poorly differentiated oral squamous cell carcinoma. On the other hand, different grades of oral squamous cell carcinoma showed a high staining index of alpha smooth muscle actin expression in the stroma. While cases of oral papillary squamous cell carcinoma were either moderate or low-staining. Oral papillary squamous cell carcinoma has a favourable prognosis compared to different histological grades, and the prognosis does not depend only on histological grade but also on other prognostic factors.
Identifiants
pubmed: 38884825
doi: 10.1007/s12105-024-01635-4
pii: 10.1007/s12105-024-01635-4
doi:
Substances chimiques
Actins
0
Biomarkers, Tumor
0
Transforming Growth Factor beta
0
ACTA2 protein, human
0
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
47Informations de copyright
© 2024. The Author(s).
Références
Givony S (2020) Oral squamous cell carcinoma (OSCC) an overview. J Med Sci 8:67–74
Nair S, Singh B, Pawar PV, Datta S, Nair D, Kane S, Chaturvedi P (2016) Squamous cell carcinoma of tongue and buccal mucosa: clinico-pathologically different entities. Eur Arch Otorhinolaryngol 273(11):3921–3928
doi: 10.1007/s00405-016-4051-0
pubmed: 27098612
Warnakulasuriya S, Kujan O, Aguirre-Urizar JM, Bagan JV, González-Moles MÁ, Kerr AR, Johnson NW (2021) Oral potentially malignant disorders: a consensus report from an international seminar on nomenclature and classification, convened by the WHO collaborating centre for oral cancer. Oral Dis 27(8):1862–1880
doi: 10.1111/odi.13704
pubmed: 33128420
Padma R, Kalaivani A, Sundaresan S, Sathish P (2017) The relationship between histological differentiation and disease recurrence of primary oral squamous cell carcinoma. J Oral Maxillofacial Pathol: JOMFP 21(3):461
doi: 10.4103/jomfp.JOMFP_241_16
pmcid: 5763883
Alotaiby F, Song F, Boyce BJ, Cao D, Zhao Y, Lai J (2018) Unusual papillary squamous cell carcinoma of the tip of tongue presenting in a patient status post heart transplant. Anticancer Res 38(7):4203–4206
doi: 10.21873/anticanres.12715
pubmed: 29970551
Serra A, Caltabiano R, Scalia G, Palmucci S, Di Mauro P, Cocuzza S (2017) Papillary squamous cell carcinoma of the palatine tonsil: a rare cancer of the head and neck. Carcinoma squamoso papillare della tonsilla palatina: un raro tumore della testa e del collo. Acta Otorhinolaryngol Italica 37(4):341–345. https://doi.org/10.14639/0392-100X-1281
doi: 10.14639/0392-100X-1281
Almangush A, Mäkitie AA, Triantafyllou A, de Bree R, Strojan P, Rinaldo A, Leivo I (2020) Staging and grading of oral squamous cell carcinoma: an update. Oral Oncol 107:104799
doi: 10.1016/j.oraloncology.2020.104799
pubmed: 32446214
Sounni NE, Noel A (2013) Targeting the tumor microenvironment for cancer therapy. Clin Chem 59(1):85–93
doi: 10.1373/clinchem.2012.185363
pubmed: 23193058
Xing F, Saidou J, Watabe K (2010) Cancer associated fibroblasts (CAFs) in tumor microenvironment. Front Biosci 15:166
doi: 10.2741/3613
pmcid: 2905156
Kajdaniuk D, Marek B, Borgiel-Marek H, Kos-Kudła B (2013) Transforming growth factor beta1 (TGFbeta1) in physiology and pathology. Endokrynol Pol 64(5):384–396
doi: 10.5603/EP.2013.0022
pubmed: 24186596
Kadoya K, Togo S, Tulafu M, Namba Y, Iwai M, Watanabe J, Takahashi K (2019) Specific features of fibrotic lung fibroblasts highly sensitive to fibrotic processes mediated via TGF-β-ERK5 interaction. Cell Physiol Biochem 52(4):822–837
doi: 10.33594/000000057
pubmed: 30946557
Chen J, Peters A, Papke CL, Villamizar C, Ringuette LJ, Cao J, Milewicz DM (2017) Loss of smooth muscle α-actin leads to NF-κB–dependent increased sensitivity to angiotensin II in smooth muscle cells and aortic enlargement. Circul Res 120(12):1903–1915
doi: 10.1161/CIRCRESAHA.117.310563
Shinde AV, Humeres C, Frangogiannis NG (2017) The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling. Biochim Biophys Acta Mol Basis Dis 1863(1):298–309
doi: 10.1016/j.bbadis.2016.11.006
pubmed: 27825850
Wick MR (2019) The hematoxylin and eosin stain in anatomic pathology—an often-neglected focus of quality assurance in the laboratory. Semin Diagn Pathol 36(5):303–311
doi: 10.1053/j.semdp.2019.06.003
pubmed: 31230963
Magaki S, Hojat SA, Wei B, So A, Yong WH (2019) An introduction to the performance of immunohistochemistry. Biobanking: methods and protocols. Humana Press, New York, pp 289–298
doi: 10.1007/978-1-4939-8935-5_25
Jakobsson PÅ, Eneroth CM, Killander D, Moberger G, Mårtensson B (1973) Histologic classification and grading of malignancy in carcinoma of the larynx. Acta Radiol Ther Phys Biol 12(1):1–8
doi: 10.3109/02841867309131085
pubmed: 4725642
Brandwein-Gensler M, Teixeira MS, Lewis CM, Lee B, Rolnitzky L, Hille JJ, Wang BY (2005) Oral squamous cell carcinoma: histologic risk assessment, but not margin status, is strongly predictive of local disease-free and overall survival. Am J Surg Pathol 29(2):167–178
doi: 10.1097/01.pas.0000149687.90710.21
pubmed: 15644773
Prasad BV, Kakatkar GS, Jain P, Jain M, Patel M, Khan J (2016) Expression of myofibroblasts in oral squamous cell carcinoma: an immunohistochemical study. J Contemp Dent Pract 17(10):857–860
doi: 10.5005/jp-journals-10024-1944
pubmed: 27794159
Almangush A, Mäkitie AA, Triantafyllou A, de Bree R, Strojan P, Rinaldo A, Leivo I (2020) Staging and grading of oral squamous cell carcinoma: an update. Oral Oncol 107:104799
doi: 10.1016/j.oraloncology.2020.104799
pubmed: 32446214
Vered M, Shnaiderman-Shapiro A, Zlotogorski-Hurvitz A, Salo T, Yahalom R (2019) Cancer-associated fibroblasts in the tumor microenvironment of tongue carcinoma is a heterogeneous cell population. Acta Histochem 121(8):151446
doi: 10.1016/j.acthis.2019.151446
pubmed: 31604589
Bao Z, Yang X, Shi L, Feng J, Liu W, Zhou Z (2012) Clinicopathologic features of oral squamous papilloma and papillary squamous cell carcinoma: a study of 197 patients from eastern China. Ann Diagn Pathol 16(6):454–458
doi: 10.1016/j.anndiagpath.2012.04.001
pubmed: 22627075
Ding Y, Ma L, Shi L, Feng J, Liu W, Zhou Z (2013) Papillary squamous cell carcinoma of the oral mucosa: a clinicopathologic and immunohistochemical study of 12 cases and literature review. Ann Diagn Pathol 17(1):18–21
doi: 10.1016/j.anndiagpath.2012.04.005
pubmed: 22672806
Mukdad L, Heineman TE, Alonso J, Badran KW, Kuan EC, St John MA (2019) Oral tongue squamous cell carcinoma survival as stratified by age and sex: a surveillance, epidemiology, and end results analysis. Laryngoscope 129(9):2076–2081. https://doi.org/10.1002/lary.27720
doi: 10.1002/lary.27720
pubmed: 30575045
Sopik V, Narod SA (2018) The relationship between tumour size, nodal status and distant metastases: on the origins of breast cancer. Breast Cancer Res Treat 170:647–656
doi: 10.1007/s10549-018-4796-9
pubmed: 29693227
pmcid: 6022519
Almangush A, Pirinen M, Heikkinen I, Mäkitie AA, Salo T, Leivo I (2018) Tumour budding in oral squamous cell carcinoma: a meta-analysis. Br J Cancer 118(4):577–586
doi: 10.1038/bjc.2017.425
pubmed: 29190636
Xu B, Salama AM, Valero C, Yuan A, Khimraj A, Saliba M, Ghossein R (2021) The prognostic role of histologic grade, worst pattern of invasion, and tumor budding in early oral tongue squamous cell carcinoma: a comparative study. Virchows Archiv. https://doi.org/10.1007/s00428-021-03063-z
doi: 10.1007/s00428-021-03063-z
pubmed: 34618230
pmcid: 10126990
Aurello P, Berardi G, Giulitti D, Palumbo A, Tierno SM, Nigri G, Ramacciato G (2017) Tumor-Stroma Ratio is an independent predictor for overall survival and disease free survival in gastric cancer patients. Surgeon 15(6):329–335
doi: 10.1016/j.surge.2017.05.007
pubmed: 28629870
Xu B, Salama AM, Valero C, Yuan A, Khimraj A, Saliba M, Zanoni DK, Ganly I, Patel SG, Katabi N, Ghossein R (2021) The prognostic role of histologic grade, worst pattern of invasion, and tumor budding in early oral tongue squamous cell carcinoma: a comparative study. Virchows Archiv 479(3):597–606. https://doi.org/10.1007/s00428-021-03063-z
doi: 10.1007/s00428-021-03063-z
pubmed: 33661329
pmcid: 8417140
Angadi PV, Patil PV, Hallikeri K, Mallapur MD, Hallikerimath S, Kale AD (2015) Tumor budding is an independent prognostic factor for prediction of lymph node metastasis in oral squamous cell carcinoma. Int J Surg Pathol 23(2):102–110. https://doi.org/10.1177/1066896914565022
doi: 10.1177/1066896914565022
pubmed: 25559273
Karpathiou G, Vieville M, Gavid M, Camy F, Dumollard JM, Magné N, Froudarakis M, Prades JM, Peoc’h M (2019) Prognostic significance of tumor budding, tumor-stroma ratio, cell nests size, and stroma type in laryngeal and pharyngeal squamous cell carcinomas. Head Neck 41(6):1918–1927. https://doi.org/10.1002/hed.25629
doi: 10.1002/hed.25629
pubmed: 30620425
Al-Rawi NH, Kawas SA, Ani MA, Alnuaimi AS, El-Sayed W, Alrashdan MS (2023) Prediction of lymphovascular and perineural invasion of oral squamous cell carcinoma by combined expression of p63 and Cyclin D1. Eur J Dent 17(4):1170–1178. https://doi.org/10.1055/s-0042-1760301
doi: 10.1055/s-0042-1760301
pubmed: 36716784
pmcid: 10795001
Deepthi G, Shyam NDVN, Kumar GK, Narayen V, Paremala K, Preethi P (2020) Characterization of perineural invasion in different histological grades and variants of oral squamous cell carcinoma. J Oral Maxillofac Pathol 24(1):57–63. https://doi.org/10.4103/jomfp.JOMFP_162_19
doi: 10.4103/jomfp.JOMFP_162_19
pubmed: 32508449
pmcid: 7269278
Karatsaidis A, Schreurs O, Axéll T, Helgeland K, Schenck K (2003) Inhibition of the transforming growth factor-beta/Smad signaling pathway in the epithelium of oral lichen. J Invest Dermatol 121(6):1283–1290. https://doi.org/10.1046/j.1523-1747.2003.12633.x
doi: 10.1046/j.1523-1747.2003.12633.x
pubmed: 14675171
Bacman D, Merkel S, Croner R, Papadopoulos T, Brueckl W, Dimmler A (2007) TGF-beta receptor 2 downregulation in tumour-associated stroma worsens prognosis and high-grade tumours show more tumour-associated macrophages and lower TGF-beta1 expression in colon carcinoma: a retrospective study. BMC Cancer 7(1):1–8
doi: 10.1186/1471-2407-7-156
Chen Y, Di C, Zhang X, Wang J, Wang F, Yan JF, Zhang H (2020) Transforming growth factor β signaling pathway: a promising therapeutic target for cancer. J Cell Physiol 235(3):1903–1914
doi: 10.1002/jcp.29108
pubmed: 31332789
Verrecchia F, Rédini F (2018) Transforming growth factor-β signaling plays a pivotal role in the interplay between osteosarcoma cells and their microenvironment. Front Oncol 8:133
doi: 10.3389/fonc.2018.00133
pubmed: 29761075
pmcid: 5937053
Orgen Calli A, Dere Y, Sari A, Dirilenoglu F, Onur I, İmre K (2019) Evaluation of stromal myofibroblasts in laryngeal dysplasia and invasive squamous cell carcinoma. Indian J Otolaryngol Head Neck Surg 71:233–238
doi: 10.1007/s12070-018-01572-z
pubmed: 31741965
pmcid: 6848344
Jayaraj G, Sherlin HJ, Ramani P, Premkumar P, Natesan A (2015) Stromal myofibroblasts in oral squamous cell carcinoma and potentially malignant disorders. Indian J Cancer 52(1):87–92
doi: 10.4103/0019-509X.175580
pubmed: 26837985
Gandhi V, Mahajan A, Kansal YP (2023) An immunohistochemical analysis for evaluating the diagnostic role of myofibroblasts in oral squamous cell carcinoma using α-smooth muscle actin antibody. Adv Biomed Res 12(1):123
doi: 10.4103/abr.abr_160_21
pubmed: 37434928
pmcid: 10331533
Gadbail AR, Chaudhary MS, Sarode SC, Gondivkar SM, Belekar L, Mankar-Gadbail MP, Patil S (2019) Ki67, CD105 and α-smooth muscle actin expression in disease progression model of oral submucous fibrosis. J Invest Clin Dent 10(4):e12443
doi: 10.1111/jicd.12443
Parajuli H, Teh MT, Abrahamsen S, Christoffersen I, Neppelberg E, Lybak S, Costea DE (2017) Integrin α11 is overexpressed by tumour stroma of head and neck squamous cell carcinoma and correlates positively with alpha smooth muscle actin expression. J Oral Pathol Med 46(4):267–275
doi: 10.1111/jop.12493
pubmed: 27699902
Rodrigues RM, Bernardo VG, Da Silva SD, Camisasca DR, Faria PADS, Dias FL, Lourenço SDQC (2019) How pathological criteria can impact prognosis of tongue and floor of the mouth squamous cell carcinoma. J Appl Oral Sci 28:e2019198
Bariety J, Hill GS, Mandet C, Irinopoulou T, Jacquot C, Meyrier A, Bruneval P (2003) Glomerular epithelial–mesenchymal transdifferentiation in pauci-immune crescentic glomerulonephritis. Nephrol Dial Transplant 18(9):1777–1784
doi: 10.1093/ndt/gfg231
pubmed: 12937224
Storch KN, Taatjes DJ, Bouffard NA, Locknar S, Bishop NM, Langevin HM (2007) Alpha smooth muscle actin distribution in cytoplasm and nuclear invaginations of connective tissue fibroblasts. Histochem Cell Biol 127:523–530
doi: 10.1007/s00418-007-0275-9
pubmed: 17310383
Batra V, Musani AI, Hastie AT, Khurana S, Carpenter KA, Zangrilli JG, Peters SP (2004) Bronchoalveolar lavage fluid concentrations of transforming growth factor (TGF)-β1, TGF-β2, interleukin (IL)-4 and IL-13 after segmental allergen challenge and their effects on α-smooth muscle actin and collagen III synthesis by primary human lung fibroblasts. Clin Exp Allergy 34(3):437–444
doi: 10.1111/j.1365-2222.2004.01885.x
pubmed: 15005738
Gupta K (2022) An Immunohistochemical study on expression of Laminin5 Gamma2 chain and alpha smooth muscle actin in oral squamous cell carcinoma. Sch J Dent Sci 1:14–20
doi: 10.36347/sjds.2022.v09i01.003
Qiu J, Jiang E, Shang Z (2022) Prognostic value of tumor-stroma ratio in oral squamous cell carcinoma: contribution of cancer associated fibroblasts