DUSP6 inhibition overcomes neuregulin/HER3-driven therapy tolerance in HER2+ breast cancer.

BCI FOXM1 Lapatinib Neratinib Non-genetic Drug Tolerance

Journal

EMBO molecular medicine
ISSN: 1757-4684
Titre abrégé: EMBO Mol Med
Pays: Germany
ID NLM: 101487380

Informations de publication

Date de publication:
17 Jun 2024
Historique:
received: 04 09 2023
accepted: 24 05 2024
revised: 08 05 2024
medline: 18 6 2024
pubmed: 18 6 2024
entrez: 17 6 2024
Statut: aheadofprint

Résumé

Despite clinical benefits of tyrosine kinase inhibitors (TKIs) in cancer, most tumors can reactivate proliferation under TKI therapy. Here we present transcriptional profiling of HER2+ breast cancer cells transitioning from dormant drug tolerant cells to re-proliferating cells under continuous HER2 inhibitor (HER2i) therapy. Focusing on phosphatases, expression of dual-specificity phosphatase DUSP6 was found inhibited in dormant cells, but strongly induced upon regrowth. DUSP6 expression also selectively associated with poor patient survival in HER2+ breast cancers. DUSP6 overexpression conferred apoptosis resistance, whereas its pharmacological blockade prevented therapy tolerance development under HER2i therapy. DUSP6 targeting also synergized with clinically used HER2i combination therapies. Mechanistically DUSP6 is a positive regulator of HER3 expression, and its impact on HER2i tolerance was mediated by neuregulin-HER3 axis. In vivo, genetic targeting of DUSP6 reduced tumor growth in brain metastasis model, whereas its pharmacological targeting induced synthetic lethal therapeutic effect in combination with HER2i. Collectively this work demonstrates that DUSP6 drives escape from HER2i-induced dormancy, and that DUSP6 is a druggable target to overcome HER3-driven TKI resistance.

Identifiants

pubmed: 38886591
doi: 10.1038/s44321-024-00088-0
pii: 10.1038/s44321-024-00088-0
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s).

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Auteurs

Majid Momeny (M)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland. majid.momeny@uth.tmc.edu.
The Brown Foundation Institute of Molecular Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, USA. majid.momeny@uth.tmc.edu.

Mari Tienhaara (M)

Medicity Research Laboratories, Faculty of Medicine, University of Turku, Turku, Finland.
Institute of Biomedicine, University of Turku, Turku, Finland.

Mukund Sharma (M)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Institute of Biomedicine, University of Turku, Turku, Finland.

Deepankar Chakroborty (D)

Medicity Research Laboratories, Faculty of Medicine, University of Turku, Turku, Finland.
Institute of Biomedicine, University of Turku, Turku, Finland.

Roosa Varjus (R)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.

Iina Takala (I)

Medicity Research Laboratories, Faculty of Medicine, University of Turku, Turku, Finland.
Institute of Biomedicine, University of Turku, Turku, Finland.

Joni Merisaari (J)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.

Artur Padzik (A)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.

Andreas Vogt (A)

University of Pittsburgh Drug Discovery Institute, Department of Computational and Systems Biology, Pittsburgh Technology Center, Pittsburgh, PA, USA.

Ilkka Paatero (I)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.

Klaus Elenius (K)

Medicity Research Laboratories, Faculty of Medicine, University of Turku, Turku, Finland.
Institute of Biomedicine, University of Turku, Turku, Finland.

Teemu D Laajala (TD)

Department of Mathematics and Statistics, University of Turku, Turku, Finland.

Kari J Kurppa (KJ)

Medicity Research Laboratories, Faculty of Medicine, University of Turku, Turku, Finland.
Institute of Biomedicine, University of Turku, Turku, Finland.

Jukka Westermarck (J)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland. jukwes@utu.fi.
Institute of Biomedicine, University of Turku, Turku, Finland. jukwes@utu.fi.

Classifications MeSH