A hybrid structure based on silk fibroin/PVA nanofibers and alginate/gum tragacanth hydrogel embedded with cardamom extract.
Alginates
/ chemistry
Nanofibers
/ chemistry
Fibroins
/ chemistry
Polyvinyl Alcohol
/ chemistry
Hydrogels
/ chemistry
Tragacanth
/ chemistry
Plant Extracts
/ chemistry
Anti-Bacterial Agents
/ pharmacology
Elettaria
/ chemistry
Animals
Escherichia coli
/ drug effects
Mice
Staphylococcus aureus
/ drug effects
Biocompatible Materials
/ chemistry
Cardamom extract
Hybrid structure
Hydrogel
Silk fibroin nanofibers
Tragacanth
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 Jun 2024
18 Jun 2024
Historique:
received:
08
03
2024
accepted:
24
05
2024
medline:
19
6
2024
pubmed:
19
6
2024
entrez:
18
6
2024
Statut:
epublish
Résumé
Hybrid structures made of natural-synthetic polymers have been interested due to high biological features combining promising physical-mechanical properties. In this research, a hybrid dressing consisting of a silk fibroin (SF)/polyvinyl alcohol (PVA) nanofibers and sodium alginate (SA)/gum tragacanth (GT) hydrogel incorporating cardamom extract as an antibacterial agent was prepared. Accordingly, SF was extracted from cocoons followed by electrospinning in blend form with PVA (SF/PVA ratio: 1:1) under the voltage of 18 kV and the distances of 15 cm. The SEM images confirmed the formation of uniform, bead free fibers with the average diameter of 199 ± 28 nm. FTIR and XRD results revealed the successful extraction of SF and preparation of mixed fibrous mats. Next, cardamom oil extract-loaded SA/GT hydrogel was prepared and the nanofibrous structure was placed on the surface of hydrogel. SEM analysis depicted the uniform morphology of hybrid structure with desirable matching between two layers. TGA analysis showed desired thermal stability. The swelling ratio was found to be 1251% after 24 h for the hybrid structure and the drug was released without any initial burst. MTT assay and cell attachment results showed favorable biocompatibility and cell proliferation on samples containing extract, and antibacterial activity values of 85.35% against S. aureus and 75% against E. coli were obtained as well. The results showed that the engineered hybrid nanofibrous-hydrogel film structure incorporating cardamom oil extract could be a promising candidate for wound healing applications and skin tissue engineering.
Identifiants
pubmed: 38890349
doi: 10.1038/s41598-024-63061-4
pii: 10.1038/s41598-024-63061-4
doi:
Substances chimiques
Alginates
0
Fibroins
9007-76-5
Polyvinyl Alcohol
9002-89-5
Hydrogels
0
Tragacanth
9000-65-1
Plant Extracts
0
Anti-Bacterial Agents
0
Biocompatible Materials
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
14010Informations de copyright
© 2024. The Author(s).
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