Antibody Production and Immunoassay Development for Authenticating Chlorpheniramine Maleate Adulteration in Herbal Tea.
adulteration
chlorphenamine maleate
herbal teas
molecular simulation
Journal
Foods (Basel, Switzerland)
ISSN: 2304-8158
Titre abrégé: Foods
Pays: Switzerland
ID NLM: 101670569
Informations de publication
Date de publication:
22 May 2024
22 May 2024
Historique:
received:
17
04
2024
revised:
14
05
2024
accepted:
16
05
2024
medline:
19
6
2024
pubmed:
19
6
2024
entrez:
19
6
2024
Statut:
epublish
Résumé
Chlorphenamine maleate is a prohibited additive found in herbal teas and health foods. Excessive intake of this substance can result in adverse health effects. In this study, two novel haptens, PEM and bepotastine (PB1), mimicking chlorphenamine maleate structure were designed and synthesized based on molecular simulation for developing two corresponding polyclonal antibodies (PEM-Ab and PB1-Ab), respectively. Afterward, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed to quickly and accurately detect chlorphenamine maleate in herbal teas using PB1-Ab, which has a high sensitivity and specificity. For chlorphenamine maleate, the half-maximal inhibitory concentration (IC
Identifiants
pubmed: 38890838
pii: foods13111609
doi: 10.3390/foods13111609
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Key Research and Development Program of China
ID : 2023YFF1105103, 2023YFF1104200, 2023YFF1104700
Organisme : Guangdong Marine Economic Development Program for Six Categories of Marine Industries
ID : no. GDNRC[2023]36
Organisme : Guangdong Natural Science Foundation
ID : 2023A1515011834
Organisme : Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Province
ID : 2023KJ130
Organisme : Zhongshan Science and Technology Innovation Team
ID : no. CXTD2022004