Expression of Fascin and SALL4 in odontogenic cysts and tumors: an immunohistochemical appraisal.

Humans Transcription Factors / metabolism Microfilament Proteins / metabolism Odontogenic Cysts / metabolism Carrier Proteins / metabolism Immunohistochemistry Ameloblastoma / metabolism Odontogenic Tumors / metabolism Biomarkers, Tumor / metabolism
Ameloblastoma Fascin Immunohistochemistry Odontogenic cysts Odontogenic tumors SALL4

Journal

F1000Research
ISSN: 2046-1402
Titre abrégé: F1000Res
Pays: England
ID NLM: 101594320

Informations de publication

Date de publication:
2022
Historique:
accepted: 18 06 2024
medline: 19 6 2024
pubmed: 19 6 2024
entrez: 19 6 2024
Statut: epublish

Résumé

Various stemness markers (SOX2, OCT4, and NANOG) have been studied in odontogenic cysts and tumors. However, studies on SALL4 having similar properties of stemness has not been documented. Additionally, insight into fascin as a migratory molecule is less explored. In this study, the expression of SALL4 and fascin were evaluated in ameloblastoma, adenomatoid odontogenic tumor (AOT), odontogenic keratocyst (OKC), dentigerous cyst (DC), radicular cyst (RC), and calcifying odontogenic cyst (COC). Semi-quantitative analysis of fascin and SALL4 immuno-positive cells was done in a total of 40 cases of ameloblastoma (11 plexiform, 12 follicular, 12 unicystic, and 5 desmoplastic) variants, 6 cases of AOT, 15 each of OKC, DC, RC and 5 of COC. Chi-square test was applied to evaluate the association between SALL4 and fascin expression in odontogenic cysts and tumors. Fascin immunopositivity was observed in peripheral ameloblast-like cells, and weak or absent in stellate reticulum-like cells. A moderate to weak immune-reactivity to SALL4 was observed in the cytoplasm of ameloblastoma, epithelial cells of dentigerous and radicular cysts, having a marked inflammatory infiltrate, which is an interesting observation. COC and AOT had negative to weak expressions. No recurrence has been reported. Expression of fascin in ameloblastomas elucidate their role in motility and localized invasion. Its expression in less aggressive lesions like DC, COC, AOT will incite to explore the other functional properties of fascin. SALL4 expression in the cytoplasm of odontogenic cysts and tumors may represent inactive or mutant forms which requires further validation.

Sections du résumé

Background UNASSIGNED
Various stemness markers (SOX2, OCT4, and NANOG) have been studied in odontogenic cysts and tumors. However, studies on SALL4 having similar properties of stemness has not been documented. Additionally, insight into fascin as a migratory molecule is less explored. In this study, the expression of SALL4 and fascin were evaluated in ameloblastoma, adenomatoid odontogenic tumor (AOT), odontogenic keratocyst (OKC), dentigerous cyst (DC), radicular cyst (RC), and calcifying odontogenic cyst (COC).
Methods UNASSIGNED
Semi-quantitative analysis of fascin and SALL4 immuno-positive cells was done in a total of 40 cases of ameloblastoma (11 plexiform, 12 follicular, 12 unicystic, and 5 desmoplastic) variants, 6 cases of AOT, 15 each of OKC, DC, RC and 5 of COC. Chi-square test was applied to evaluate the association between SALL4 and fascin expression in odontogenic cysts and tumors.
Results UNASSIGNED
Fascin immunopositivity was observed in peripheral ameloblast-like cells, and weak or absent in stellate reticulum-like cells. A moderate to weak immune-reactivity to SALL4 was observed in the cytoplasm of ameloblastoma, epithelial cells of dentigerous and radicular cysts, having a marked inflammatory infiltrate, which is an interesting observation. COC and AOT had negative to weak expressions. No recurrence has been reported.
Conclusions UNASSIGNED
Expression of fascin in ameloblastomas elucidate their role in motility and localized invasion. Its expression in less aggressive lesions like DC, COC, AOT will incite to explore the other functional properties of fascin. SALL4 expression in the cytoplasm of odontogenic cysts and tumors may represent inactive or mutant forms which requires further validation.

Identifiants

DOI: 10.12688/f1000research.126091.3 PMID: 38895097 PMC: PMC11184278
pubmed: 38895097
doi: 10.12688/f1000research.126091.3
pmc: PMC11184278
doi:

Substances chimiques

SALL4 protein, human 0
fascin 146808-54-0
Transcription Factors 0
Microfilament Proteins 0
Carrier Proteins 0
Biomarkers, Tumor 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1578

Informations de copyright

Copyright: © 2023 Kulkarni S et al.

Déclaration de conflit d'intérêts

No competing interests were disclosed.

Auteurs

Spoorti Kulkarni (S)

Oral Pathology and Microbiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, 576104, India.
ORCID: 0000-0003-3118-7595

Harishanker Alampally (H)

Oral Pathology and Microbiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, 576104, India.

Vasudev Guddattu (V)

Department of Data Science, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.

Gabriel Rodrigues (G)

Department of General Surgery, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.

Sunitha Carnelio (S)

Oral Pathology and Microbiology, Manipal College of Dental Sciences, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, 576104, India.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Maladies du système stomatognathique › Maladies de la mâchoire › Kystes de la mâchoire › Kystes odontogènes › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Immunohistochimie › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Tumeurs odontogènes › Améloblastome › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Tumeurs odontogènes › Expression of Fascin and SALL4 in odontogenic...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Expression of Fascin and SALL4 in odontogenic...