Bacteriocins attenuate Listeria monocytogenes-induced intestinal barrier dysfunction and inflammatory response.
Listeria monocytogenes
/ drug effects
Bacteriocins
/ pharmacology
Humans
Caco-2 Cells
Anti-Bacterial Agents
/ pharmacology
Inflammation
NF-kappa B
/ metabolism
Bacterial Adhesion
/ drug effects
Tight Junction Proteins
/ metabolism
Cytokines
/ metabolism
Listeriosis
/ microbiology
Cell Movement
/ drug effects
Antibacterial activity
Bacteriocin
Inflammatory responses
Intestinal epithelial barrier
Virulence factors
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
19 Jun 2024
19 Jun 2024
Historique:
received:
24
01
2024
accepted:
06
06
2024
revised:
02
06
2024
medline:
19
6
2024
pubmed:
19
6
2024
entrez:
19
6
2024
Statut:
epublish
Résumé
Bacteriocins have the potential to effectively improve food-borne infections or gastrointestinal diseases and hold promise as viable alternatives to antibiotics. This study aimed to explore the antibacterial activity of three bacteriocins (nisin, enterocin Gr17, and plantaricin RX-8) and their ability to attenuate intestinal barrier dysfunction and inflammatory responses induced by Listeria monocytogenes, respectively. Bacteriocins have shown excellent antibacterial activity against L. monocytogenes without causing any cytotoxicity. Bacteriocins inhibited the adhesion and invasion of L. monocytogenes on Caco-2 cells, lactate dehydrogenase (LDH), trans-epithelial electrical resistance (TEER), and cell migration showed that bacteriocin improved the permeability of Caco-2 cells. These results were attributed to the promotion of tight junction proteins (TJP) assembly, specifically zonula occludens-1 (ZO-1), occludin, and claudin-1. Furthermore, bacteriocins could alleviate inflammation by inhibiting the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) pathways and reducing the secretion of interleukin-6 (IL-6), interleukin-1 β (IL-1β) and tumor necrosis factor α (TNF-α). Among three bacteriocins, plantaricin RX-8 showed the best antibacterial activity against L. monocytogenes and the most pronounced protective effect on the intestinal barrier due to its unique structure. Based on our findings, we hypothesized that bacteriocins may inhibit the adhesion and invasion of L. monocytogenes by competing adhesion sites. Moreover, they may further enhance intestinal barrier function by inhibiting the expression of L. monocytogenes virulence factors, increasing the expression of TJP and decreasing the secretion of inflammatory factors. Therefore, bacteriocins will hopefully be an effective alternative to antibiotics, and this study provides valuable insights into food safety concerns. KEY POINTS: • Bacteriocins show excellent antibacterial activity against L. monocytogenes • Bacteriocins improve intestinal barrier damage and inflammatory response • Plantaricin RX-8 has the best protective effect on Caco-2 cells damage.
Identifiants
pubmed: 38896287
doi: 10.1007/s00253-024-13228-w
pii: 10.1007/s00253-024-13228-w
doi:
Substances chimiques
Bacteriocins
0
Anti-Bacterial Agents
0
NF-kappa B
0
Tight Junction Proteins
0
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
384Informations de copyright
© 2024. The Author(s).
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