A label-free and rapid fluorometric strategy for microRNA detection using CRISPR-Cas12a coupled with copper nanoparticles.
CRISPR-Cas12a
DNA-CuNPs
Fluorescent biosensing
miRNA
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
19 Jun 2024
19 Jun 2024
Historique:
received:
02
05
2024
accepted:
07
06
2024
medline:
19
6
2024
pubmed:
19
6
2024
entrez:
19
6
2024
Statut:
epublish
Résumé
CRISPR-Cas12a with robust trans-cleavage activity were employed to mitigate background fluorescence signal, achieving sensitive detection of miRNA-21. The activation of trans-cleavage activity of Cas12a was achieved by utilizing cDNA as a trigger. Upon the presence of target miRNA-21, cDNA hybridizes with it forming a DNA/RNA double-stranded structure. Exonuclease III (ExoIII) facilitates the degradation of cDNA, releasing the target for subsequent cycles. Due to cDNA degradation, the trans-cleavage activity of Cas12a remains unactivated and does not disrupt the synthesis template of copper nanoparticles. Addition of Cu
Identifiants
pubmed: 38896292
doi: 10.1007/s00604-024-06496-w
pii: 10.1007/s00604-024-06496-w
doi:
Substances chimiques
Copper
789U1901C5
MicroRNAs
0
MIRN21 microRNA, human
0
Cas12a protein
EC 3.1.-
CRISPR-Associated Proteins
0
Bacterial Proteins
0
Endodeoxyribonucleases
EC 3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
405Subventions
Organisme : Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi
ID : 2020KY12012
Organisme : Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi
ID : 2023KY0522
Organisme : Natural Science Foundation of Guangxi Province
ID : 2021GXNSFBA076001
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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