Delineating the Acquired Genetic Diversity and Multidrug Resistance in Alcaligenes from Poultry Farms and Nearby Soil.

Antibiotic Resistance Environment Genomic Island Horizontal Gene Transfer Multidrug-Resistant Alcaligenes faecalis Whole Genome Sequencing

Journal

Journal of microbiology (Seoul, Korea)

ISSN: 1976-3794

Titre abrégé: J Microbiol

Pays: Korea (South)

ID NLM: 9703165

Informations de publication

Date de publication:
21 Jun 2024

Historique:

received: 08 12 2023

accepted: 22 02 2024

revised: 25 01 2024

medline: 21 6 2024

pubmed: 21 6 2024

entrez: 21 6 2024

Statut: aheadofprint

Résumé

Alcaligenes faecalis is one of the most important and clinically significant environmental pathogens, increasing in importance due to its isolation from soil and nosocomial environments. The Gram-negative soil bacterium is associated with skin endocarditis, bacteremia, dysentery, meningitis, endophthalmitis, urinary tract infections, and pneumonia in patients. With emerging antibiotic resistance in A. faecalis, it has become crucial to understand the origin of such resistance genes within this clinically significant environmental and gut bacterium. In this research, we studied the impact of antibiotic overuse in poultry and its effect on developing resistance in A. faecalis. We sampled soil and faecal materials from five poultry farms, performed whole genome sequencing & analysis and identified four strains of A. faecalis. Furthermore, we characterized the genes in the genomic islands of A. faecalis isolates. We found four multidrug-resistant A. faecalis strains that showed resistance against vancomycin (MIC >1000 μg/ml), ceftazidime (50 μg/ml), colistin (50 μg/ml) and ciprofloxacin (50 μg/ml). From whole genome comparative analysis, we found more than 180 resistance genes compared to the reference sequence. Parts of our assembled contigs were found to be similar to different bacteria which included pbp1A and pbp2 imparting resistance to amoxicillin originally a part of Helicobacter and Bordetella pertussis. We also found the Mycobacterial insertion element IS6110 in the genomic islands of all four genomes. This prominent insertion element can be transferred and induce resistance to other bacterial genomes. The results thus are crucial in understanding the transfer of resistance genes in the environment and can help in developing regimes for antibiotic use in the food and poultry industry.

Identifiants

DOI: 10.1007/s12275-024-00129-w PMID: 38904697

pubmed: 38904697

doi: 10.1007/s12275-024-00129-w

pii: 10.1007/s12275-024-00129-w

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : Council for Scientific and Industrial Research

ID : OLP-2035

Organisme : Council for Scientific and Industrial Research

ID : OLP-2081

Organisme : DST-SERB

ID : GPP-0423

Informations de copyright

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