Rapid bacterial evaluation beyond the colony forming unit in osteomyelitis.
CFU
Staphylococcus aureus
bacteria quantification
colony forming unit
diagnosis
human
infectious disease
microbiology
osteocyte
osteomyelitis
staphylococcus
staphylococcus aureus
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
24 Jun 2024
24 Jun 2024
Historique:
medline:
24
6
2024
pubmed:
24
6
2024
entrez:
24
6
2024
Statut:
epublish
Résumé
Examination of bacteria/host cell interactions is important for understanding the aetiology of many infectious diseases. The colony forming unit (CFU) has been the standard for quantifying bacterial burden for the past century, however, this suffers from low sensitivity and is dependent on bacterial culturability in vitro. Our data demonstrate the discrepancy between the CFU and bacterial genome copy number in an osteomyelitis-relevant co-culture system and we confirm diagnosis and quantify bacterial load in clinical bone specimens. This study provides an improved workflow for the quantification of bacterial burden in such cases.
Identifiants
pubmed: 38910553
doi: 10.7554/eLife.93698
pii: 93698
doi:
pii:
Banques de données
Dryad
['10.5061/dryad.s4mw6m9f5']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Health and Medical Research Council
ID : 2011042
Organisme : University of Adelaide
ID : Postgraduate Research Scholarship
Informations de copyright
© 2024, Sun et al.
Déclaration de conflit d'intérêts
QS, KH, DM, NG, AZ, LS, GA, DY No competing interests declared